Nitrate reductase activity of plasma membranes from cultured carrot cells

PROTOPLASMA ◽  
1995 ◽  
Vol 184 (1-4) ◽  
pp. 151-157 ◽  
Author(s):  
R. Barr ◽  
M. B�ttger ◽  
F. L. Crane ◽  
D. J. Morr�
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Plasma Membranes ◽  
Reductase Activity ◽  
Carrot Cells

scholarly journals Relationships between nitrate uptake and nitrate reductase activity in Cucumis sativus L.

2014 ◽  
Vol 67 (3-4) ◽  
pp. 253-257
Author(s):  
Grażyna Kłobus ◽  
Jolanta Jerzykiewicz ◽  
Józef Buczek
Keyword(s):  
Plasma Membrane ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Plasma Membranes ◽  
Polyclonal Antibodies ◽  
Reductase Activity ◽  
Nitrate Transport ◽  
Plasma Membrane Vesicles ◽  
Cucumis Sativus L

Anti-NR IgG fragments obtained after papain digestion of polyclonal antibodies gave the positive immunological reaction with both, a soluble and plasma membrane-bound nitrate reductase. Anti-NR antibody as well as IgG fragments almost totally inhibited the nitrate reductase activity in cytosol proving a crossreaction of antibody with the catalytic site of a soluble NR. Anti-NR IgG fragments, but not undigested polyclonal antibodies affected the activity of the nitrate reductase associated with plasma membranes. Discrepancy in the action of intact antibodies and fragments obtained after they digestion were interpreted as a consequence of same differences in the ability of those molecules to the penetration through the membrane. Undigested anti-NR antibody have no effect on the nitrate uptake by intact plants, as well as by the right-side plasma membrane vesicles. On the other hand, IgG fragments of polyclonal antibodies abolished almost totally the nitrate uptake in the case of intact seedlings, but have only slight effect on the N03 uptake in plasma membranes. On the basis of above findings, some relations between nitrate uptake and its assimilation inside the cell are suggested. Since IgG fragments only slightly changed the N03 absorption in vesicles whereas the activity of plasmalemma associated nitrate reductase was strongly repressed, we concluded that the PM-NR is not structurally involved in the nitrate transport through the membrane.

Nitrate Reductase Activity of Maize Hybrids and Their Parental Inbred 1

Crop Science ◽  
1966 ◽  
Vol 6 (2) ◽  
pp. 169-173 ◽  
Author(s):  
L. E. Schrader ◽  
D. M. Peterson ◽  
E. R. Leng ◽  
R. H. Hageman
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Maize Hybrids ◽  
Parental Inbred

Chromosomal Control of Nitrate Reductase Activity in Hexaploid Wheat 1

Crop Science ◽  
1982 ◽  
Vol 22 (1) ◽  
pp. 85-88 ◽  
Author(s):  
E. L. Deckard ◽  
N. D. Williams ◽  
J. J. Hammond ◽  
L. R. Joppa
Keyword(s):  
Nitrate Reductase ◽  
Hexaploid Wheat ◽  
Nitrate Reductase Activity ◽  
Reductase Activity

scholarly journals Antagonism of chloride and nitrate inhibits nitrate reductase activity in chloride-stressed maize

2021 ◽  
Author(s):  
Xudong Zhang ◽  
Bastian L. Franzisky ◽  
Lars Eigner ◽  
Christoph‐Martin Geilfus ◽  
Christian Zörb
Keyword(s):  
Zea Mays ◽  
Polyethylene Glycol ◽  
Nitrate Reductase ◽  
Osmotic Stress ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Shoot Biomass ◽  
Lower Intensity ◽  
Maize Variety ◽  
Osmotic Balance

AbstractChloride (Cl−) is required for photosynthesis and regulates osmotic balance. However, excess Cl− application negatively interacts with nitrate ($${\mathrm{NO}}_{3}^{-}$$ NO 3 - ) uptake, although its effect on $${\mathrm{NO}}_{3}^{-}$$ NO 3 - metabolism remains unclear. The aim was to test whether Cl− stress disturbs nitrate reductase activity (NRA). A maize variety (Zea mays L. cv. LG 30215) was hydroponically cultured in a greenhouse under the following conditions: control (2 mM CaCl2), moderate Cl− (10 mM CaCl2), high Cl− (60 mM CaCl2). To substantiate the effect of Cl− stress further, an osmotic stress with lower intensity was induced by 60 g polyethylene glycol (PEG) 6000 L−1 + 2 mM CaCl2), which was 57% of the osmotic pressure being produced by 60 mM CaCl2. Results show that high Cl− and PEG-induced osmotic stress significantly reduced shoot biomass, stomatal conductance and transpiration rate, but NRA was only decreased by high Cl− stress. The interference of NRA in chloride-stressed maize is supposed to be primarily caused by the antagonistic uptake of Cl− and $${\mathrm{NO}}_{3}^{-}$$ NO 3 - .

Development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains

2017 ◽  
Vol 199 (6) ◽  
pp. 863-873 ◽  
Author(s):  
Silvana Gomes dos Santos ◽  
Flaviane da Silva Ribeiro ◽  
Camila Sousa da Fonseca ◽  
Willian Pereira ◽  
Leandro Azevedo Santos ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity
Sign in / Sign up

Export Citation Format

Share Document