Histochemical localization of enzyme activity during floral evocation in the shoot apical meristem ofSinapis alba

PROTOPLASMA ◽  
1978 ◽  
Vol 94 (3-4) ◽  
pp. 315-324 ◽  
Author(s):  
A. Jacqmard
Keyword(s):  
Enzyme Activity ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Histochemical Localization ◽  
Floral Evocation

scholarly journals Sample preparation for laser-microdissection of soybean shoot apical meristem

2012 ◽  
Vol 3 (1) ◽  
pp. 3 ◽  
Author(s):  
Chui E. Wong ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Sample Preparation ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Laser Microdissection ◽  
Specific Cell ◽  
Legume Crop ◽  
Continuous Formation ◽  
Molecular Events ◽  
Depth Analysis ◽  
Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

Planta ◽  
2002 ◽  
Vol 214 (6) ◽  
pp. 829-836 ◽  
Author(s):  
Andreas Mordhorst ◽  
Marijke Hartog ◽  
Mazen El Tamer ◽  
Thomas Laux ◽  
Sacco de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Apical Meristem ◽  
Apical Meristem
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