The flagellar apparatus ofDunaliella: Isolation of basal body-flagellar root complex

PROTOPLASMA ◽  
1985 ◽  
Vol 127 (1-2) ◽  
pp. 82-92 ◽  
Author(s):  
Francelyne Marano ◽  
Angelica Santa-Maria ◽  
Sushila Krishnawamy
Keyword(s):  
Basal Body ◽  
Flagellar Apparatus

scholarly journals Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli.

1978 ◽  
Vol 134 (2) ◽  
pp. 655-667 ◽  
Author(s):  
Y Komeda ◽  
M Silverman ◽  
P Matsumura ◽  
M Simon
Keyword(s):  
Escherichia Coli ◽  
Basal Body ◽  
Flagellar Apparatus

MKS1, encoding a component of the flagellar apparatus basal body proteome, is mutated in Meckel syndrome

2006 ◽  
Vol 38 (2) ◽  
pp. 155-157 ◽  
Author(s):  
Mira Kyttälä ◽  
Jonna Tallila ◽  
Riitta Salonen ◽  
Outi Kopra ◽  
Nicolai Kohlschmidt ◽  
...  
Keyword(s):  
Basal Body ◽  
Flagellar Apparatus ◽  
Meckel Syndrome

scholarly journals Cell Division of Giardia intestinalis: Flagellar Developmental Cycle Involves Transformation and Exchange of Flagella between Mastigonts of a Diplomonad Cell

2006 ◽  
Vol 5 (4) ◽  
pp. 753-761 ◽  
Author(s):  
Eva Nohýnková ◽  
Pavla Tůmová ◽  
Jaroslav Kulda
Keyword(s):  
Cell Division ◽  
Basal Body ◽  
Light And Electron Microscopy ◽  
Flagellar Apparatus ◽  
Eukaryotic Cell ◽  
Giardia Intestinalis ◽  
Developmental Cycle ◽  
Maturation Process ◽  
Pathogenic Potential ◽  
Microtubular Cytoskeleton

ABSTRACT Giardia intestinalis is a binucleated diplomonad possessing four pairs of flagella of distinct location and function. Its pathogenic potential depends on the integrity of a complex microtubular cytoskeleton that undergoes a profound but poorly understood reorganization during cell division. We examined the cell division of G. intestinalis with the aid of light and electron microscopy and immunofluorescence methods and present here new observations on the reorganization of the flagellar apparatus in the dividing Giardia. Our results demonstrated the presence of a flagellar maturation process during which the flagella migrate, assume different position, and transform to different flagellar types in progeny until their maturation is completed. For each newly assembled flagellum it takes three cell cycles to become mature. The mature flagellum of Giardia is the caudal one that possesses a privileged basal body at which the microtubules of the adhesive disk nucleate. In contrast to generally accepted assumption that each of the two diplomonad mastigonts develops separately, we found that they are developmentally linked, exchanging their cytoskeletal components at the early phase of mitosis. The presence of the flagellar maturation process in a metamonad protist Giardia suggests that the basal body or centriole maturation is a universal phenomenon that may represent one of the core processes in a eukaryotic cell.

scholarly journals DEVELOPMENT OF THE FLAGELLAR APPARATUS OF NAEGLERIA

1966 ◽  
Vol 31 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Allan D. Dingle ◽  
Chandler Fulton
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Basal Body ◽  
Flagellar Apparatus ◽  
Basal Bodies ◽  
Naegleria Gruberi

Flagellates of Naegleria gruberi have an interconnected flagellar apparatus consisting of nucleus, rhizoplast and accessory filaments, basal bodies, and flagella. The structures of these components have been found to be similar to those in other flagellates. The development of methods for obtaining the relatively synchronous transformation of populations of Naegleria amebae into flagellates has permitted a study of the development of the flagellar apparatus. No indications of rhizoplast, basal body, or flagellum structures could be detected in amebae. A basal body appears and assumes a position at the cell surface with its filaments perpendicular to the cell membrane. Axoneme filaments extend from the basal body filaments into a progressive evagination of the cell membrane which becomes the flagellum sheath. Continued elongation of the axoneme filaments leads to differentiation of a fully formed flagellum with a typical "9 + 2" organization, within 10 min after the appearance of basal bodies.

Ultrastructure and development of the flagellar apparatus and flagellar motion in the colonial graeen alga Astrephomene gubernaculifera

1983 ◽  
Vol 63 (1) ◽  
pp. 21-41
Author(s):  
H.J. Hoops ◽  
G.L. Floyd
Keyword(s):  
Basal Body ◽  
Rotational Symmetry ◽  
Amorphous Material ◽  
Flagellar Apparatus ◽  
The Other ◽  
Basal Bodies ◽  
Mature Cell ◽  
Embryonic Cleavage ◽  
Development Proceeds ◽  
Concurrent Events

Immediately following embryonic cleavage, the cells of Astrephomene have four equal-sized basal bodies, two of which are connected by a striated distal fibre and two striated proximal fibres. The four microtubular rootlets, which alternate between having 3/1 and 2 members, are arranged cruciately. The two basal bodies that are connected by the striated fibres then extend into flagella, while the two accessory basal bodies are now markedly shorter. At this stage the flagellar apparatus has 180 degrees rotational symmetry and is very similar to the flagellar apparatus of the unicellular Chlamydomonas and related algae. Development proceeds with a number of concurrent events. The basal bodies begin to separate at their proximal ends and become nearly parallel. Each striated proximal fibre detaches at one end from one of the basal bodies. Each half of the flagellar apparatus, which consists of a flagellum and attached basal body, an accessory basal body, two rootlets and a striated fibre (formerly one of the proximal striated fibres), rotates about 90 degrees, the two halves rotating in opposite directions. An electron-dense strut forms near one two-membered rootlet and grows past both basal bodies. During this time a fine, fibrous component appears between newly developed spade-like structures and associated amorphous material connected to each basal body. The basal bodies continue to separate as the distal fibre stretches and finally detaches from one of them. These processes result in the loss of the 180 degree rotational symmetry present in previous stages. Although the flagella continue to separate, there is no further reorganization of the components of the flagellar apparatus. In the mature cell of Astrephomene, the two flagella are inserted separately and are parallel. The four microtubular rootlets are no longer arranged cruciately. Three of the rootlets are nearly parallel, while the fourth is approximately perpendicular to the other three. A straited fibre connects each basal body to the underside of the strut. These fibres run in the direction of the effective stroke of the flagella and might be important either in anchoring the basal bodies or in the initiation of flagellar motion. Unlike the case in the unicellular Chlamydomonas, the two flagella beat in the same direction and in parallel planes. The flagella of a given cell may or may not beat in synchrony. The combination of this type of flagellar motion and the parallel, separate flagella appears to be suited to the motion of this colonial organism.

Cellular asymmetry in Chlamydomonas reinhardtii

1989 ◽  
Vol 94 (2) ◽  
pp. 273-285 ◽  
Author(s):  
J.A. Holmes ◽  
S.K. Dutcher
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Basal Body ◽  
Flagellar Apparatus ◽  
Spindle Pole ◽  
The Other ◽  
Unicellular Alga ◽  
Phototactic Behavior ◽  
Cellular Asymmetry ◽  
Asymmetric Fusion ◽  
Two Sides

Although largely bilaterally symmetric, the two sides of the unicellular alga Chlamydomonas reinhardtii can be distinguished by the location of the single eyespot. When viewed from the anterior end, the eyespot is always closer to one flagellum than the other, and located at an angle of approximately 45 degrees clockwise of the flagellar plane. This location correlates with the position of one of four acetylated microtubule bundles connected to the flagellar apparatus. Each basal body is attached to two of these microtubule rootlets. The rootlet that positions the eyespot is always attached to the same basal body, which is the daughter of the parental/daughter basal body pair. At mitosis, the replicated basal body pairs segregate in a precise orientation that maintains the asymmetry of the cell and results in mitotic poles that have an invariant handedness. The fusion of gametic cells during mating is also asymmetric. As a result of asymmetric, but different, locations of the plus and minus mating structures, mating preferentially results in quadriflagellate dikaryons with parallel flagellar pairs and both eyespots on the same side of the cell. This asymmetric fusion, as well as all the other asymmetries described, may be necessary for the proper phototactic behavior of these cells. The invariant handedness of the spindle pole, eyespot position, and mating structure position appears to be based on the inherent asymmetry of the basal body pair, providing an example of how an intracellular pattern can be determined and maintained.

The ultrastructure of Ulothrix mucosa. II. The flagellar apparatus of the zoospore

1986 ◽  
Vol 64 (1) ◽  
pp. 166-176 ◽  
Author(s):  
G. M. Lokhorst ◽  
W. Star
Keyword(s):  
Basal Body ◽  
Spatial Configuration ◽  
Rotational Symmetry ◽  
Flagellar Apparatus ◽  
Taxonomic Status ◽  
Proximal Region ◽  
Basal Bodies ◽  
Serial Sectioning ◽  
Counterclockwise Direction ◽  
Microtubular Roots

The actual spatial configuration of the flagellar apparatus of the quadriflagellate zoospore of Ulothrix mucosa Thuret has been reconstructed by serial sectioning analysis. This apparatus shows an architecture quite similar to that found in related Ulvophyceae. Common characteristics are the differently leveled basal body pairs; the 180° rotational symmetry of the flagellar apparatus; the proximal overlap of the upper basal bodies which are displaced with respect to each other in the counterclockwise direction; terminal caps; four cruciately arranged microtubular roots (R2, R4); a distinctly striated distal connecting fibre that interconnects the upper basal bodies; and striated bands (SB1) that join the R4s to the lower basal bodies. Specific features are the arrangement of the R4 in a three over one configuration when entering the proximal region of the flagellar apparatus; the differently shaped proximal sheaths and their association with a proximal sheath connecting band; the presence of two system II fibres (rhizoplasts) which arise from the lower basal body pair; the striated bands (SB2) that connect the R2s to the lower basal bodies; the distinct striation of the system I fibre, which is not only intimately associated with the R2, but also with the R4 (not earlier reported for an ulvophycean alga); and, finally, the relevant displacement of the lower basal body pair in a counterclockwise direction of approximately half a basal body diameter. In light of these findings the taxonomic status of the Ulotrichales as well as of the Ulvophyceae is discussed.

scholarly journals Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy

2005 ◽  
Vol 4 (7) ◽  
pp. 1253-1263 ◽  
Author(s):  
Stefan Geimer ◽  
Michael Melkonian
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Immunoelectron Microscopy ◽  
Basal Body ◽  
Flagellar Apparatus ◽  
Single Copy ◽  
Asymmetric Structure ◽  
Transitional Region ◽  
Ef Hand ◽  
Basal Apparatus ◽  
Postembedding Immunoelectron Microscopy

ABSTRACT In the flagellate green alga Chlamydomonas reinhardtii the Ca2+-binding EF-hand protein centrin is encoded by a single-copy gene. Previous studies have localized the protein to four distinct structures in the flagellar apparatus: the nucleus-basal body connector, the distal connecting fiber, the flagellar transitional region, and the axoneme. To explain the disjunctive distribution of centrin, the interaction of centrin with as yet unknown specific centrin-binding proteins has been implied. Here, we demonstrate using serial section postembedding immunoelectron microscopy of isolated cytoskeletons that centrin is located in additional structures (transitional fibers and basal body lumen) and that the centrin-containing structures of the basal apparatus are likely part of a continuous filamentous scaffold that extends from the nucleus to the flagellar bases. In addition, we show that centrin is located in the distal lumen of the basal body in a rotationally asymmetric structure, the V-shaped filament system. This novel centrin-containing structure has also been detected near the distal end of the probasal bodies. Taken together, these results suggest a role for a rotationally asymmetric centrin “seed” in the growth and development of the centrin scaffold following replication of the basal apparatus.

Observations on the ultrastructure of the choanoflagellate Codosiga botrytis (Ehr.) Saville-Kent with special reference to the flagellar apparatus

1975 ◽  
Vol 17 (1) ◽  
pp. 191-219 ◽  
Author(s):  
D.J. Hibberd
Keyword(s):  
Basal Body ◽  
Complex Structure ◽  
Flagellar Apparatus ◽  
Transitional Zone ◽  
Dense Material ◽  
Electron Dense Material ◽  
Phylogenetic Implications ◽  
Dense Band ◽  
Interstitial Material ◽  
Flagellar Appendages

The ultrastructure of the choanoflagellate Codosiga botrytis is described with particular reference to the flagellar appendages, the flagellar rootlet system, the transition zone, the basal body and accessory centrioles, and the stalk. The controversial early reports of flagellar appendages in this species have been confirmed and they have been detected in 2 further species, Salpingoeca frequentissima and Monosiga sp. The appendages consist of a delicate bilateral vane 2 mum wide on either side of the axis, composed of extremely fine overlapping or interwoven fibrils. The flagellar root system consists of a large number of radiating microtubules associated with bands of electron-dense material near the basal body; striated roots are absent. The microtubules extend from several separate foci, those in any one group originating near a composite electron-dense band, and for a distance of 300 nm from the basal body they are separated by blocks of interstitial material. The flagellar basal body forms one of a diplosome pair of centrioles. The triplet microtubules of the accessory centriole are embedded in amorphous electron-dense material and the whole is enveloped in a sheath of similar appearance. The existence of a third centriole close to the diplosome pair is also reported. The relatively complex structure of the flagellar transitional zone is described. The stalk is composed of a core of circular lacunae, which may or may not contain finger-like protoplasmic extensions of the posterior end of the cell, surrounded by a continuation of the sheath material which encloses the remainder of the protoplast. In the stalk only there is a further closely sheathing layer about 15 nm thick which is regularly striated, the spacing of the striations in shadowcast material and sections being about 3 times that measured by negative staining. The structure of choanoflagellates differs widely from that of the algal class Chrysophyceae, the group in which they are included in some classifications, and from the remainder of the algae; they do not appear to have a place in either the algae or the plant kingdom. The structure of Codosiga botrytis is briefly compared with that of sponge choanocytes and collared cells in the Metazoa and some of the possible phylogenetic implications of this are indicated.

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