Induction of de novo synthesis of phenylalanine ammonia-lyase by L-?-aminooxy-?-phenylpropionic acid in suspension cultures ofDaucus carota L.

Planta ◽  
1982 ◽  
Vol 154 (5) ◽  
pp. 454-458 ◽  
Author(s):  
Wolfgang No� ◽  
Hanns Ulrich Seitz
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
De Novo ◽  
Suspension Cultures ◽  
De Novo Synthesis ◽  
Phenylpropionic Acid

Elicitor-Induced Formation of Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Suspension Cultures from Constitutive Isoflavone Conjugates upon Inhibition of Phenylalanine Ammonia Lyase

1991 ◽  
Vol 46 (1-2) ◽  
pp. 43-50 ◽  
Author(s):  
Ulrike Mackenbrock ◽  
Wolfgang Barz
Keyword(s):  
Cell Suspension ◽  
Cicer Arietinum ◽  
Phenylalanine Ammonia Lyase ◽  
De Novo ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phenylpropionic Acid ◽  
Cicer Arietinum L ◽  
Physiological Conditions ◽  
L Cell

After inhibition of phenylalanine ammonia lyase by L- α-aminooxy-β-phenylpropionic acid, the constitutively formed formononetin 7-O-glucoside-6″-O-malonate is metabolized with the isoflavone aglycone being used as an intermediate in the elicitor-induced formation of pterocarpan phytoalexins in chickpea cell suspension cultures. In elicited cultures not treated with the inhibitor phytoalexins are synthesized de novo from phenylalanine. Therefore, in chickpea cells the constitutive isoflavone conjugate metabolism and the elicitor-induced pterocarpan formation show metabolic linkage under specific physiological conditions.

Lipoxygenase isoforms in elicitor-treated parsley cell suspension cultures

2000 ◽  
Vol 28 (6) ◽  
pp. 827-829 ◽  
Author(s):  
C. Noehringer ◽  
D. Scheel ◽  
E. Blée
Keyword(s):  
Jasmonic Acid ◽  
Cell Suspension ◽  
Cell Cultures ◽  
De Novo ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Defence Response ◽  
Fungal Elicitor ◽  
De Novo Synthesis

Treatment of parsley cell cultures with a fungal elicitor triggered the induction of a lipoxygenase isoform which may be involved in the de novo synthesis of defence-response inducers, such as jasmonic acid or 12-oxo-phytodienoic acid.

scholarly journals Wound-induced Phenolic Accumulation and Browning in Lettuce (Lactucasativa L.) Leaf Tissue is Reduced by Exposure to n-alcohols

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1131B-1131
Author(s):  
Mikal Saltveit ◽  
Youngjun Choi ◽  
Francisco Tomás-Barberán
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
De Novo ◽  
Physiological Responses ◽  
Leaf Tissue ◽  
De Novo Synthesis ◽  
Phenolic Metabolism ◽  
Pal Activity ◽  
Wound Signal ◽  
Fresh Cut ◽  
Tissue Browning

A wound signal originates at the site of injury in lettuce [Lactucasativa (L.)] leaf tissue and propagates into adjacent tissue where it induces a number of physiological responses that include increased phenolic metabolism with the de novo synthesis of phenylalanine ammonia lyase (PAL, EC 4.3.1.5), the synthesis and accumulation of soluble phenolic compounds (e.g., chlorogenic acid), and subsequent tissue browning. Exposing excised mid-rib leaf tissue to vapors (20 μmol·g-1 FW) or aqueous solutions (100 mm) of n-alcohols inhibited this wound-induced tissue browning by 40% and 60%, respectively. Effectiveness of the alcohol increased linearly from ethanol to the seven-carbon heptanol, and then was lost for the longer n-alcohols 1-octanol and 1-nonanol. The 2- and 3-isomers of the effective alcohols did not significantly reduce wound-induced phenolic accumulation at optimal 1-alcohol concentrations, but significant reductions did occur at much higher concentrations (100 μmol·g-1 FW) of the 2-, and 3-isomers. The active n-alcohols were maximally effective when applied during the first 2 h after excision, and were ineffective if applied 12 h after excision. Phospholipase D (PLD) and its product phosphatidic acid (PA) are thought to initiate the oxylipin pathway that culminates in the production of jasmonic acid, and PLD is specifically inhibited by 1-butanol, but not by 2- or 3-butanol. These results suggest that PLD, PA, and the oxylipin pathway may be involved in producing the wound signal responsible for increased wound-induced PAL activity, phenolic accumulation, and browning in fresh-cut lettuce leaf tissue.

Wall regeneration by plasmolysed cells from tissue suspension cultures of Sphaerocarpos donnellii

1980 ◽  
Vol 43 (1) ◽  
pp. 167-175
Author(s):  
M.A. Grusak ◽  
R.J. Thomas ◽  
B.H. Marsh
Keyword(s):  
Liquid Scintillation ◽  
De Novo ◽  
Radioactive Tracer ◽  
Suspension Cultures ◽  
Cellulose Microfibrils ◽  
Wall Material ◽  
De Novo Synthesis ◽  
Tracer Techniques ◽  
Cross Linkage ◽  
Compositional Changes

De novo synthesis of wall material by cells of Sphaerocarpos donnellii was followed using radioactive tracer techniques. Uniform uptake of [14C]glucose by plasmolysed suspension cultures was demonstrated autoradiographically. Liquid scintillation counts of acid- or alkali-soluble wall carbohydrates indicate that hemicellulose and cellulose make up the major wall fractions in control and plasmolysed tissues. The amount of label in walls of plasmolysed cells was significantly lower than in those of controls. Higher relative radioactivity in pectin and hemicellulose, and lower radioactivity in cellulose were found in plasmolysed tissue. These compositional changes may enhance wall stability by providing numerous sites for cross-linkage between cellulose microfibrils of regenerated walls.

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