Combined effects of carbon monoxide plus sodium nitrite or carbon tetrachloride on the sciatic motor conduction velocity of rats

1975 ◽  
Vol 35 (1) ◽  
pp. 81-88 ◽  
Author(s):  
D. Pankow ◽  
W. Ponsold ◽  
W. Glatzel ◽  
K. Tietze
1993 ◽  
Vol 21 (1) ◽  
pp. 57-64
Author(s):  
Roberta Ferrara ◽  
Michela Rezzadore ◽  
Stefano Cazzaro ◽  
Roberto Tolando ◽  
Maurizio Manno

The reductive metabolism of carbon tetrachloride (CC14) by human haemoglobin (Hb) was observed in vitro by absolute absorption spectra recorded under anaerobic conditions. The following results were obtained: 1) a decrease of the 430nm peak typical of free reduced Hb (Hb2+); 2) the formation of a shoulder of absorbance, attributable to the production of a complex between Hb2+ and a metabolite of CC14 carbon monoxide (Hb-CO); and 3) the oxidation of some Hb2+ to methaemoglobin (Hb3+). The concentration of these three forms — Hb2+, Hb-CO and Hb3+ — during anaerobic incubation of Hb with CC14 was calculated algebraically from the absolute spectra. CO production was then calculated from the concentration of Hb-CO, using a suitable calibration curve. Interestingly, under identical experimental conditions, a substrate-dependent loss of Hb-derived haem, but not of Hb itself nor of haem-derived porphyrin fluorescence, was measured. Preliminary HPLC studies to clarify the discrepancy and, in particular, the role and fate of the haem group, showed two substrate-dependent modified haem products. The results indicate that human Hb is able to catalyse the reductive activation of CCl4, and suggest that, during the process, its prosthetic group haem may be modified by CC14 metabolites to products which maintain a tetrapyrrolic structure but are unable to react with pyridine.


2001 ◽  
Vol 24 (7) ◽  
pp. 966-968 ◽  
Author(s):  
R. Jon L. Walters ◽  
Nicholas M.F. Murray

Biochemistry ◽  
1991 ◽  
Vol 30 (10) ◽  
pp. 2713-2719 ◽  
Author(s):  
Ute E. Krone ◽  
Rudolf K. Thauer ◽  
Harry P. C. Hogenkamp ◽  
Klaus Steinbach

Author(s):  
Lucas B. Fontanesi ◽  
Frederico S. Fazan ◽  
Fernando J. Dias ◽  
Maria Cristina L. Schiavoni ◽  
Wilson Marques Jr. ◽  
...  

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