Ultrastructural changes in the anterior horn synapses of rat spinal cord under different locomotor conditions

1978 ◽  
Vol 42 (1) ◽  
pp. 9-21 ◽  
Author(s):  
L. Cheresharov ◽  
W. Ovtscharoff ◽  
S. Manolov
Keyword(s):  
Spinal Cord ◽  
Anterior Horn ◽  
Ultrastructural Changes ◽  
Rat Spinal Cord

scholarly journals Sirtuin 1 (SIRT1) and activated caspase 3 expression on rat spinal cord in acute phase after Sciatic nerve injury

2018 ◽  
Vol 18 (1) ◽  
pp. 50-56
Author(s):  
Dwi Nur Ahsani ◽  
Rina Susilowati ◽  
Yustina Andwi Ari Sumiwi
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Acute Phase ◽  
Caspase 3 ◽  
Sciatic Nerve Injury ◽  
Posterior Horn ◽  
Anterior Horn ◽  
Rat Spinal Cord ◽  
Sirt1 Expression

Background: Increase of SIRT1 expression inhibit the increase of activated Caspase 3 expression in acute phase of traumatic brain injury (TBI). However, the SIRT1 expression on rat spinal cord in acute phase after peripheral nerve injury was unknown. Objective: To reveal SIRT1and activated Caspase 3 expression on rat spinal cord in acute phase after sciatic nerve injury and to reveal the correlation between SIRT1 and activated Caspase 3 expression after sciatic nerve injury. Method: Thirty male Wistar rats aged 3 months were divided into sham operated and crush injury group. Termination was performing at days 3, 7 and 14. Lumbar spinal cord segments 4-5 (VTh12-VL1 high) embedded in paraffin blocks. Imunohistochemistry staining was performed using antibody of SIRT1 nuclear marker and activated Caspase 3. Observations were performed by two double blinded observers in 400x magnification. The data were analyzed using unpaired t-test and Pearson correlation. All data were processed using statistical analysis with confidence interval (CI) 95% and limit of significance (p-values)<0.05. Results: SIRT1 expression on the anterior horn was higher compared with control at days 7 and 14 (p<0.05), but no differences were found on posterior horn at all termination days(p>0.05). Activated Caspase 3 expression on the anterior horn was higher at all termination days(p<0.05) and at day 7 on posterior horn (p<0.05). Thereis a positive correlation between SIRT1 and activated Caspase 3 expression on anterior horn at day 7 (p<0.05). Conclusion: SIRT1 expression on the anterior horn was higher compared with the control at days 7 and 14. Activated Caspase 3 expression on the anterior horn was higher on all termination days and at day 7 on posterior horn. There is a positive correlation between SIRT1 and activated Caspase 3 expression on anterior horn on day 7. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.50-56 

An Early Developmental Marker for Radial Glia in Rat Spinal Cord

1996 ◽  
Vol 54 ◽  
pp. 36-37
Author(s):  
V. Kriho ◽  
H.-Y. Yang ◽  
C.-M. Lue ◽  
N. Lieska ◽  
G. D. Pappas
Keyword(s):  
Spinal Cord ◽  
Intermediate Filament ◽  
Radial Glia ◽  
Rat Spinal Cord ◽  
Future Studies ◽  
Spinal Cord Development ◽  
Median Septum ◽  
Differentiation Marker ◽  
Early Developmental ◽  
Developing Rat

Radial glia have been classically defined as those early glial cells that radially span their thin processes from the ventricular to the pial surfaces in the developing central nervous system. These radial glia constitute a transient cell population, disappearing, for the most part, by the end of the period of neuronal migration. Traditionally, it has been difficult to definitively identify these cells because the principal criteria available were morphologic only.Using immunofluorescence microscopy, we have previously defined a phenotype for radial glia in rat spinal cord based upon the sequential expression of vimentin, glial fibrillary acidic protein and an intermediate filament-associated protein, IFAP-70/280kD. We report here the application of another intermediate filament-associated protein, IFAP-300kD, originally identified in BHK-21 cells, to the immunofluorescence study of radial glia in the developing rat spinal cord.Results showed that IFAP-300kD appeared very early in rat spinal cord development. In fact by embryonic day 13, IFAP-300kD immunoreactivity was already at its peak and was observed in most of the radial glia which span the spinal cord from the ventricular to the subpial surfaces (Fig. 1). Interestingly, from this time, IFAP-300kD immunoreactivity diminished rapidly in a dorsal to ventral manner, so that by embryonic day 16 it was detectable only in the maturing macroglial cells in the marginal zone of the spinal cord and the dorsal median septum (Fig. 2). By birth, the spinal cord was essentially immuno-negative for this IFAP. Thus, IFAP-300kD appears to be another differentiation marker available for future studies of gliogenesis, especially for the early stages of radial glia differentiation.

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