Investigation of the nonstationary stage in operation of air-drying units using the method of short-cycle nonheated adsorption

1976 ◽  
Vol 12 (7) ◽  
pp. 596-599
Author(s):  
V. I. Ermakov ◽  
Yu. I. Shumyatskii ◽  
M. B. Orlovskaya ◽  
A. I. Sidorov ◽  
N. S. Torocheshnikov
Keyword(s):  
Air Drying ◽  
Short Cycle ◽  
Nonstationary Stage

Parametric Study on Separation Performance of Air Drying PSA and Application of Short Cycle Time Approximation.

2000 ◽  
Vol 33 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Hongmei Lou ◽  
Fei Dong ◽  
Yoshiro Tominaga ◽  
Akio Kodama ◽  
Motonobu Goto ◽  
...  
Keyword(s):  
Cycle Time ◽  
Parametric Study ◽  
Separation Performance ◽  
Air Drying ◽  
Short Cycle ◽  
Time Approximation ◽  
Short Cycle Time

Solution, exchangeable and clay-fixed ammonium in south coast British Columbia soils

1996 ◽  
Vol 76 (4) ◽  
pp. 473-483 ◽  
Author(s):  
C. G. Kowalenko ◽  
S. Yu
Keyword(s):  
British Columbia ◽  
Biological Significance ◽  
South Coast ◽  
Relative Importance ◽  
Limited Information ◽  
Extractant Concentration ◽  
Air Drying ◽  
Relative Significance ◽  
Short Cycle ◽  
Long Cycle

Solution, exchangeable and clay-fixed ammonium were measured in a variety of south coast British Columbia soils in the laboratory using extraction, equilibration and leaching procedures, and with and without ammonium amendments in order to evaluate the relative significance of adsorption and fixation processes on nitrogen behavior. Non-amended soils contained from 59 to 224 g N g−1 of fixed ammonium and these amounts were correlated with clay (positive) and sand (negative) contents. The amount of native fixed ammonium in the soils was influenced by management history. Recovery of ammonium added to eight selected samples by a small volume of solution followed by air drying was the same with 1 and 2 M KCl extractions, but less was extracted by 0.1 M KCl and even less by water. This showed that both 1 and 2 M KCl had sufficient K+ to displace the NH4+ present (inherent and added) on the exchange sites without an apparent effect on fixed ammonium. Up to 68% of the ammonium added was not extracted by 2 M KCl and assumed to have been fixed in the clay lattice. The proportion of the added ammonium that was adsorbed onto the exchange sites was linear up to 200 μg N g−1 application, but the amount fixed by the clays was linear to only about 100 μg N g−1 application rate. Data from studies where soil columns were leached with NH4NO3 provided only limited information on the relative importance of exchange versus fixation processes to the adsorption of ammonium. Leaching columns with a limited number of batches of NH4NO3 followed by water (short cycle) were useful for comparing the ability of different soils to adsorb and retain ammonium, but the relative importance of exchange versus fixation could not be determined. In the 18 samples of this study, the amount of NH4+ adsorbed ranged from 84 to 25% during short cycle column leachings. Different patterns of adsorption occurred among the four soil samples that were leached with a large number of batches of ammonium (long cycle), but it could not be determined whether or not these patterns were related to differences in proportions of fixation relative to exchange. Measurements showed that ammonium was fixed in the soils during the long cycle leachings but that the amounts of fixed ammonium measured were influenced by air drying the sample before analysis. Several studies with and without toluene additions showed that microbial activity must be controlled during these measurements. It was concluded that south coastal soils contained significant amounts of fixed ammonium and can fix additional ammonium. The amount of ammonium in the clay fixed pool and the potential to fix added ammonium was different in relation to the soil type, their previous management, and wetting and drying cycles. The biological significance of fixed ammonium and the fixation process must not be discounted in any nitrogen studies of these soils for proper interpretation of data. Key words: Ammonium fixation, equilibration, column leaching, microbial/enzyme inhibition, extractant concentration

The Effects of Drying Techniques on Clay-Rich Soil Texture

1974 ◽  
Vol 32 ◽  
pp. 466-467
Author(s):  
T. G. Naymik
Keyword(s):  
Critical Point ◽  
Liquid Nitrogen ◽  
Liquid Nitrogen Temperature ◽  
Drying Methods ◽  
Air Drying ◽  
Freeze Dried ◽  
Critical Point Drying ◽  
Set Up ◽  
The Relationship ◽  
Quartz Grains

Three techniques were incorporated for drying clay-rich specimens: air-drying, freeze-drying and critical point drying. In air-drying, the specimens were set out for several days to dry or were placed in an oven (80°F) for several hours. The freeze-dried specimens were frozen by immersion in liquid nitrogen or in isopentane at near liquid nitrogen temperature and then were immediately placed in the freeze-dry vacuum chamber. The critical point specimens were molded in agar immediately after sampling. When the agar had set up the dehydration series, water-alcohol-amyl acetate-CO2 was carried out. The objectives were to compare the fabric plasmas (clays and precipitates), fabricskeletons (quartz grains) and the relationship between them for each drying technique. The three drying methods are not only applicable to the study of treated soils, but can be incorporated into all SEM clay soil studies.

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

Rapid Critical Point Drying of Tissues in a Parr Bomb

1972 ◽  
Vol 30 ◽  
pp. 400-401
Author(s):  
C.A. Baechler ◽  
W. C. Pitchford ◽  
J. M. Riddle ◽  
C.B. Boyd ◽  
H. Kanagawa ◽  
...  
Keyword(s):  
Critical Point ◽  
Critical Pressure ◽  
Soft Tissues ◽  
Biological Tissues ◽  
Critical Temperatures ◽  
Air Drying ◽  
The Past ◽  
Critical Point Drying ◽  
Great Stress ◽  
Infiltration Techniques

Preservation of the topographic ultrastructure of soft biological tissues for examination by scanning electron microscopy has been accomplished in the past by using lengthy epoxy infiltration techniques, or dehydration in ethanol or acetone followed by air drying. Since the former technique requires several days of preparation and the latter technique subjects the tissues to great stress during the phase change encountered during air-drying, an alternate rapid, economical, and reliable method of surface structure preservation was developed. Turnbill and Philpott had used a fluorocarbon for the critical point drying of soft tissues and indicated the advantages of working with fluids having both moderately low critical pressures as well as low critical temperatures. Freon-116 (duPont) which has a critical temperature of 19. 7 C and a critical pressure of 432 psi was used in this study.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

A technique for protecting delicate specimens during processing

1989 ◽  
Vol 47 ◽  
pp. 982-983
Author(s):  
R.J. Mount ◽  
R.V. Harrison
Keyword(s):  
Basilar Membrane ◽  
Low Cost ◽  
Organ Of Corti ◽  
Region Of Interest ◽  
Sensory Epithelium ◽  
Physical Damage ◽  
Air Drying ◽  
Specimen Handling ◽  
Two Component

The sensory end organ of the ear, the organ of Corti, rests on a thin basilar membrane which lies between the bone of the central modiolus and the bony wall of the cochlea. In vivo, the organ of Corti is protected by the bony wall which totally surrounds it. In order to examine the sensory epithelium by scanning electron microscopy it is necessary to dissect away the protective bone and expose the region of interest (Fig. 1). This leaves the fragile organ of Corti susceptible to physical damage during subsequent handling. In our laboratory cochlear specimens, after dissection, are routinely prepared by the O-T- O-T-O technique, critical point dried and then lightly sputter coated with gold. This processing involves considerable specimen handling including several hours on a rotator during which the organ of Corti is at risk of being physically damaged. The following procedure uses low cost, readily available materials to hold the specimen during processing ,preventing physical damage while allowing an unhindered exchange of fluids.Following fixation, the cochlea is dehydrated to 70% ethanol then dissected under ethanol to prevent air drying. The holder is prepared by punching a hole in the flexible snap cap of a Wheaton vial with a paper hole punch. A small amount of two component epoxy putty is well mixed then pushed through the hole in the cap. The putty on the inner cap is formed into a “cup” to hold the specimen (Fig. 2), the putty on the outside is smoothed into a “button” to give good attachment even when the cap is flexed during handling (Fig. 3). The cap is submerged in the 70% ethanol, the bone at the base of the cochlea is seated into the cup and the sides of the cup squeezed with forceps to grip it (Fig.4). Several types of epoxy putty have been tried, most are either soluble in ethanol to some degree or do not set in ethanol. The only putty we find successful is “DUROtm MASTERMENDtm Epoxy Extra Strength Ribbon” (Loctite Corp., Cleveland, Ohio), this is a blue and yellow ribbon which is kneaded to form a green putty, it is available at many hardware stores.

Comparison of freeze-fractured yeast replicas using conventional TEM and low-voltage field-emission SEM

1989 ◽  
Vol 47 ◽  
pp. 74-75
Author(s):  
William P. Wergin ◽  
Eric F. Erbe ◽  
Terrence W. Reilly
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Field Emission ◽  
Low Voltage ◽  
Objective Lens ◽  
Specimen Preparation ◽  
Lens System ◽  
Air Drying ◽  
Preparation Techniques ◽  
Scanning Electron

Although the first commercial scanning electron microscope (SEM) was introduced in 1965, the limited resolution and the lack of preparation techniques initially confined biological observations to relatively low magnification images showing anatomical surface features of samples that withstood the artifacts associated with air drying. As the design of instrumentation improved and the techniques for specimen preparation developed, the SEM allowed biologists to gain additional insights not only on the external features of samples but on the internal structure of tissues as well. By 1985, the resolution of the conventional SEM had reached 3 - 5 nm; however most biological samples still required a conductive coating of 20 - 30 nm that prevented investigators from approaching the level of information that was available with various TEM techniques. Recently, a new SEM design combined a condenser-objective lens system with a field emission electron source.

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