Interferometric dry mass and microspectrophotometric arginine determinations on bull sperm nuclei with normal and abnormal DNA content

Chromosoma ◽  
1956 ◽  
Vol 8 (1) ◽  
pp. 73-86 ◽  
Author(s):  
Cecilie Leuchtenberger ◽  
Imants Murmanis ◽  
Lydia Murmanis ◽  
Susumu Ito ◽  
David R. Weir
Keyword(s):  
Dna Content ◽  
Dry Mass ◽  
Bull Sperm ◽  
Sperm Nuclei

scholarly journals THE DESOXYRIBONUCLEIC ACID CONTENT OF ANIMAL CELLS AND ITS EVOLUTIONARY SIGNIFICANCE

1951 ◽  
Vol 34 (4) ◽  
pp. 451-462 ◽  
Author(s):  
A. E. Mirsky ◽  
Hans Ris
Keyword(s):  
Dna Content ◽  
Acid Content ◽  
Evolutionary Significance ◽  
Desoxyribonucleic Acid ◽  
Animal Cells ◽  
Dna Contents ◽  
A Cell ◽  
Sperm Nuclei

1. Evidence is summarized for the constancy of DNA content for each set of chromosomes in the various cells of an organism. 2. The DNA contents of the egg and sperm nuclei are the same. 3. A brief survey is given of DNA contents per cell in invertebrates and vertebrates. (a) In invertebrates there is some slight evidence that when primitive and higher forms are compared the amount of DNA per cell is increased in the latter. (b) In fishes there is a tendency for the amount of DNA per cell to remain constant within the different species of a family. (c) The values of DNA per cell in lung fishes, amphibians, reptiles, and birds suggest that in the evolution of these vertebrates there has been a decline in DNA content per cell. 4. Concerning the significance of quantity of DNA per cell in vertebrates: (a) It appears not to be in proportion to the number of different genes in a cell. (b) It may be related to the number of strands in the chromosomes. (c) In homologous cells of different animals it is directly related to the mass of the cell.

scholarly journals Changes in DNA, dry mass and protein content of leaf epidermis nuclei during aging of perennial monocotyledonous plants

2014 ◽  
Vol 62 (3-4) ◽  
pp. 149-154 ◽  
Author(s):  
Hanna Kuran
Keyword(s):  
Protein Content ◽  
Dna Content ◽  
Dry Mass ◽  
Leaf Epidermis ◽  
Mass Content ◽  
Significant Percentage ◽  
Young Leaves ◽  
2C Dna Content ◽  
Dna Loss ◽  
Clivia Miniata

DNA, NYS and DNFB protein contents were measured cytophotometrically using the Feulgen method in the nuclei of the epidermis from. the basal zone of young leaves and from the basal and apical zones of old leaves in two perennial monocotyledonous species, <i>Clivia miniata</i> and <i>Rhoeo discolor</i>. Dry mass was determined interferometrically. It was shown that nuclei with a 2C DNA content dominated in both zones of old leaves, and that a significant percentage of cells with a DNA content below 2C were present. The ratio between euchromatin DNA and heterochromatin DNA indicates a greater decrease in euchromatin during aging. Changes in DNA due to real DNA loss are accompanied by decreases in NYS and DNFB stained proteins and a decrease in dry mass content correlated mainly with the decrease in the amount of NYS proteins.

scholarly journals CHANGES IN THE DNA CONTENT OF ADRENAL MEDULLA NUCLEI OF RATS INTERMITTENTLY EXPOSED TO COLD

1965 ◽  
Vol 25 (3) ◽  
pp. 415-433 ◽  
Author(s):  
Maria Pia Viola-Magni
Keyword(s):  
Aqueous Solutions ◽  
Adrenal Medulla ◽  
Dna Content ◽  
Functional Activity ◽  
Normal Values ◽  
Dry Mass ◽  
Considerable Decrease ◽  
Adult Rat ◽  
Before And After

In the adrenal medulla of rats exposed intermittently to cold (+4°C) for 100 and 300 hours, a considerable decrease (24 to 40 per cent) of the DNA content per nucleus was observed, followed by restoration to normal or above normal values within 10 days after the withdrawal of the stimulus. The findings were obtained with a scanning integrating histophotometer, and confirmed by microinterferometric investigations (on the basis of the measurement of total dry mass of nuclei isolated in aqueous medium before and after treatment with DNase) and by microchemical determinations, combined with the count of the nuclei in the homogenates. The observed decrease of DNA content cannot be attributed to errors of the methods used, nor to consequences of cellular degeneration. The available evidence seems to indicate a real decrease rather than a change in the state of a part of DNA in the nucleus in vivo whereby it becomes extractable by aqueous solutions. The restoration cannot be due to mitotic processes, which were actually never detected even with the use of colchicine, since the adrenal medulla cells in the adult rat are known to be irreversible, postmitotic cells. A correlation between the functional activity of the adrenal medulla cells and the content or state of DNA in their nuclei is demonstrated.

Equine sperm nuclei with different ploidy levels: relationship between the nuclear DNA content and the nuclear area

Andrologia ◽  
2011 ◽  
Vol 43 (4) ◽  
pp. 248-253 ◽  
Author(s):  
S. Spirito ◽  
S. Campi ◽  
M. Boquet ◽  
H. Fernández ◽  
M. Ferrari
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Nuclear Area ◽  
Ploidy Levels ◽  
Sperm Nuclei
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