A method to separate spatially the temporal sequence of axon-Schwann cell interaction during nerve regeneration

1981 ◽  
Vol 10 (2) ◽  
pp. 221-232 ◽  
Author(s):  
Michael J. Politis ◽  
Peter S. Spencer
Keyword(s):  
Schwann Cell ◽  
Nerve Regeneration ◽  
Cell Interaction ◽  
Temporal Sequence

Nerve Growth Factor Enhances Nerve Regeneration through Fibronectin Grafts

1996 ◽  
Vol 21 (4) ◽  
pp. 514-522 ◽  
Author(s):  
I. H. WHITWORTH ◽  
R. A. BROWN ◽  
C. J. DORÉ ◽  
P. ANAND ◽  
C. J. GREEN ◽  
...  
Keyword(s):  
Image Analysis ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Schwann Cell ◽  
Nerve Regeneration ◽  
Axonal Regeneration ◽  
Cell Regeneration ◽  
Nerve Growth ◽  
Computerized Image Analysis ◽  
Immunohistochemical Techniques

Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate ( P≤0.007) and volume ( P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.

Activated Schwann Cell-Like Cells on Aligned Fibrin-Poly(Lactic-Co-Glycolic Acid) Structures: A Novel Construct for Application in Peripheral Nerve Regeneration

2014 ◽  
Vol 200 (5) ◽  
pp. 287-299 ◽  
Author(s):  
Christina M.A.P. Schuh ◽  
Tatjana J. Morton ◽  
Asmita Banerjee ◽  
Christian Grasl ◽  
Heinrich Schima ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Glycolic Acid ◽  
Peripheral Nerve Regeneration

scholarly journals Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration

2021 ◽  
Vol 22 (15) ◽  
pp. 7901
Author(s):  
Luca Scaccini ◽  
Roberta Mezzena ◽  
Alessia De Masi ◽  
Mariacristina Gagliardi ◽  
Giovanna Gambarotta ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Nerve Regeneration ◽  
High Fidelity ◽  
Solvent Casting ◽  
Peripheral Nerve Injuries ◽  
Cell Shaping ◽  
Micro Pattern ◽  
Chitosan Scaffolds ◽  
Set Up ◽  
Different Levels

Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this framework, chitosan is emerging as a promising biomaterial. Here, we set up a simple and effective method for the production of micro-structured chitosan films by solvent casting, with high fidelity in the micro-pattern reproducibility. Three types of chitosan directional micro-grooved patterns, presenting different levels of symmetricity, were developed for application in nerve regenerative medicine: gratings (GR), isosceles triangles (ISO) and scalene triangles (SCA). The directional patterns were tested with a Schwann cell line. The most asymmetric topography (SCA), although it polarized the cell shaping less efficiently, promoted higher cell proliferation and a faster cell migration, both individually and collectively, with a higher directional persistence of motion. Overall, the use of micro-structured asymmetrical directional topographies may be exploited to enhance the nerve regeneration process mediated by chitosan scaffolds.

scholarly journals Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelination

2021 ◽  
Author(s):  
Ana I. Seixas ◽  
Miguel R. G. Morais ◽  
Cord Brakebusch ◽  
Jo&atildeo B. Relvas
Keyword(s):  
Schwann Cell ◽  
Schwann Cells ◽  
Rho Gtpases ◽  
Intracellular Signaling ◽  
Cell Interaction ◽  
Actin Dynamics ◽  
Specific Gene ◽  
Cortical Actin ◽  
Membrane Attachment ◽  
Cytoskeleton Dynamics

Bidirectional transmission of mechanical and biochemical signals is integral to cell-environment communication and underlies the function of Schwann cells, the myelinating glia of the peripheral nervous system. As major integrators of "outside-in" signaling, Rho GTPases link actin cytoskeleton dynamics with cellular architecture to regulate adhesion and cell deformation. Using Schwann cell-specific gene inactivation, we discovered that RhoA promotes the initiation of myelination, axonal wrapping and axial spreading of Schwann cells, and is later required to restrict myelin growth in peripheral nerves. These effects are mediated by modulation of actomyosin contractility, actin dynamics and cortical actin-membrane attachment, which collectively couple tensional forces to intracellular signaling that regulate axon-Schwann cell interaction and myelin synthesis. This work establishes RhoA as an intrinsic regulator of a biomechanical response that controls the switch of Schwann cells towards the myelinating and the homeostatic states.

A new type of Schwann cell graft transplantation to promote optic nerve regeneration in adult rats

2010 ◽  
Vol 4 (8) ◽  
pp. 581-589 ◽  
Author(s):  
Yuan Fang ◽  
Xiaofen Mo ◽  
Wenyi Guo ◽  
Meng Zhang ◽  
Peihua Zhang ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Schwann Cell ◽  
Nerve Regeneration ◽  
Adult Rats ◽  
Optic Nerve Regeneration ◽  
New Type

scholarly journals Dynein promotes sustained axonal growth and Schwann cell remodeling early during peripheral nerve regeneration

PLoS Genetics ◽  
2019 ◽  
Vol 15 (2) ◽  
pp. e1007982 ◽  
Author(s):  
Melissa Ducommun Priest ◽  
Maria F. Navarro ◽  
Juliane Bremer ◽  
Michael Granato
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Axonal Growth ◽  
Cell Remodeling

Schwann cell basal lamina and central nerve regeneration

1985 ◽  
Vol 1 ◽  
pp. S131
Author(s):  
Chizuka Ide ◽  
Satoru Onodera ◽  
Koujiro Tohyama ◽  
Reiko Yokota ◽  
Tohru Nitatori
Keyword(s):  
Schwann Cell ◽  
Nerve Regeneration ◽  
Basal Lamina
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