Separation of pineal extracts by gelfiltration

1976 ◽  
Vol 38 (1) ◽  
pp. 31-41 ◽  
Author(s):  
I. Ebels ◽  
A. E. M. Horwitz-Bresser
Keyword(s):  
Pineal Extracts

INTERACTIONS OF THE PINEAL AND EXPOSURE TO CONTINUOUS LIGHT ON ORGAN WEIGHTS OF FEMALE RATS

1961 ◽  
Vol 36 (4) ◽  
pp. 617-624 ◽  
Author(s):  
Richard Jay Wurtman ◽  
Willard Roth ◽  
Mark D. Altschule ◽  
Judith J. Wurtman
Keyword(s):  
Light Exposure ◽  
Continuous Light ◽  
Female Rats ◽  
Constant Light ◽  
Ovarian Weight ◽  
Organ Weights ◽  
Bovine Pineal Extracts ◽  
Pineal Extracts ◽  
Effect Of Light

ABSTRACT Either exposure to constant light for 80 days or pinealectomy produced similar changes in the weights of the ovaries and adrenals of female rats. These were not additive when both procedures were employed. Pinealectomy did not share with light-exposure the capacity to induce uterine hypertrophy. Rats exposed to constant light for 56 days had lighter pineals than animals kept in darkness; this decrease was not affected by administration of bovine pineal extracts. The increase in ovarian weight produced in rats by exposure to light for 56 days was prevented by bovine pineal extracts, but these extracts were without effect on the uterine hypertrophy produced under the same conditions. These data suggest that the effect of light upon the weight of the ovary is mediated via the pineal.

scholarly journals STUDIES ON THE PRESENCE OF GONADOTROPHIN-INHIBITING ACTIVITY IN HUMAN PINEAL EXTRACTS

Reproduction ◽  
1971 ◽  
Vol 26 (3) ◽  
pp. 397-399 ◽  
Author(s):  
M. A. SCHNITMAN ◽  
L. DEBELJUK
Keyword(s):  
Inhibiting Activity ◽  
Pineal Extracts

INTERRELATION BETWEEN HUMAN CHORIONIC GONADOTROPHIN INHIBITORY ACTIVITY IN PLASMA AND URINE AND THE RATE OF URINARY GONADOTROPHIN EXCRETION

1965 ◽  
Vol 33 (2) ◽  
pp. 223-231 ◽  
Author(s):  
I. YAMASHITA ◽  
JANET C. HILLMAN ◽  
M. REISS
Keyword(s):  
Urinary Excretion ◽  
Inhibitory Activity ◽  
Excretion Rate ◽  
Mentally Retarded ◽  
Physical Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Urinary Excretion Rate ◽  
Young Males ◽  
Pineal Extracts

SUMMARY The interrelation of urinary and plasma human chorionic gonadotrophin (HCG) inhibitors and the urinary excretion rate of gonadotrophin was studied in ten physically and mentally retarded young males. It was found that the HCG-inhibitory principles had no effect when assayed against the boys' own urinary gonadotrophins. The excretion rate of urinary gonadotrophin was found to be independent of the presence of urinary or plasma HCG-inhibitors, which in turn were not related to the boys' physical development. Certain similarities between the properties of the plasma and urinary HCG-inhibitor and deproteinized pineal extracts are discussed.

A Simple Assay of Mammalian Pineal Extracts

1951 ◽  
Vol 77 (4) ◽  
pp. 807-808 ◽  
Author(s):  
O. Bors ◽  
W. C. Ralston
Keyword(s):  
Pineal Extracts

SOME ANTIGONADOTROPHIC EFFECTS OF MELATONIN-FREE BOVINE PINEAL EXTRACTS IN RATS

1974 ◽  
Vol 76 (3) ◽  
pp. 438-448 ◽  
Author(s):  
Richard J. Orts ◽  
Bryant Benson ◽  
Byron F. Cook
Keyword(s):  
Gel Filtration ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Reproductive Parameters ◽  
Castrate Male ◽  
Serum Luteinizing Hormone ◽  
Bovine Pineal Extracts ◽  
Modifying Effect ◽  
Pineal Extracts

ABSTRACT Studies were made to determine if a substance other than melatonin was present in bovine pineal gland extracts which possessed properties inhibitory to some gonadotrophin-dependent reproductive parameters in rats. Bovine pineal extracts were partially purified by organic solvent extraction or by ultrafiltration and gel-filtration to exclude melatonin. When administered to rats, the extracts inhibited compensatory ovarian hypertrophy, delayed vaginal opening time and reduced the incidence of constant oestrus. Either of the melatonin-free extracts reduced significantly the concentration of serum luteinizing hormone (LH) in long-term castrate male rats. The data suggest that some substance other than melatonin present in bovine pineal glands has a modifying effect on the reproductive parameters observed probably due to its inhibitory effect on LH.

ACTION OF PINEAL EXTRACTS ON THE GONADS AND THEIR FUNCTION

1963 ◽  
Vol 27 (1) ◽  
pp. 107-118 ◽  
Author(s):  
M. REISS ◽  
R. H. DAVIS ◽  
M. B. SIDEMAN ◽  
I. MAUER ◽  
E. S. PLICHTA
Keyword(s):  
Inhibitory Effect ◽  
Laboratory Animals ◽  
Genital System ◽  
Weight Changes ◽  
Test Animal ◽  
Short Term ◽  
Trichloracetic Acid ◽  
Pineal Extracts ◽  
Phosphorus Turnover

SUMMARY The action of pineal extracts on the genital system of several species of laboratory animals has been investigated. Determination of the phosphorus turnover in the genital organs of treated animals proved to be a significant guide to the action of pineal preparations. Doses of pineal extracts lower than those required for weight changes sufficed to produce changes in phosphorus metabolism. Apparently contradictory results encountered originally were explained when findings were evaluated on the basis of the age of the test animal and the type of extract used. Thus, in immature and/or hypophysectomized animals, crude aqueous extracts stimulate the genital system, whereas in mature intact animals the same extract often has an inhibitory effect on the same organs. The inhibitory effect could be measured also in immature mice treated with gonadotrophic hormone and this proved useful in short-term experiments. Crude glandular suspensions apparently contain both inhibitory and stimulatory principles. Partial separation of these was achieved through the use of trichloracetic acid, the supernatant fluid having predominantly inhibitory action while the stimulatory substance was found mainly in the precipitate.

Separation of pineal extracts on sephadex G-10

1970 ◽  
Vol 32 (1) ◽  
pp. 65-73 ◽  
Author(s):  
M. G. M. Balemans ◽  
I. Ebels ◽  
D. M. A. Vonk-Visser
Keyword(s):  
Pineal Extracts

EFFECTS OF SHEEP AND YOUNG CALF PINEAL EXTRACTS AND CONTINUOUS LIGHT ON THE PINEAL GLAND, THE GONADS AND THE OESTROUS CYCLE OF THE RAT

1968 ◽  
Vol 57 (4) ◽  
pp. 585-594 ◽  
Author(s):  
N. Prop ◽  
I. Ebels
Keyword(s):  
Pineal Gland ◽  
Continuous Light ◽  
Secretory Activity ◽  
Young Female ◽  
Oestrous Cycle ◽  
Water Soluble ◽  
Female Rats ◽  
Pineal Extract ◽  
Water Soluble Extract ◽  
Pineal Extracts

ABSTRACT Injections of a water-soluble pineal extract into young female rats kept under a normal light/dark rhythm caused a relatively high frequency of abnormal nuclei in the pineal gland. This nuclear response suggests a secretory activity in normal pinealocytes. No effect of the pineal extract on the reproductive system was observed. In females exposed to continuous light, neither the water-soluble extract nor the acetone-soluble pineal material interfered with the light induced alterations in the oestrous cycle, the uterine and ovarian weights, the condition of the pineal nuclei and the quantity of pineal lipids.

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