Large GABA cells of chick ectostriatum: anatomical evidence suggesting a double GABAergic disinhibitory mechanism. An electron microscopic immunocytochemical study

1991 ◽  
Vol 20 (6) ◽  
pp. 518-528 ◽  
Author(s):  
A. Csillag
Keyword(s):  
Immunocytochemical Study ◽  
Electron Microscopic ◽  
Anatomical Evidence

Spindle cell carcinoma (pseudosarcoma) of the anus: a light, electron microscopic and immunocytochemical study of a case

1985 ◽  
Vol 9 (9) ◽  
pp. 987-994 ◽  
Author(s):  
N. K. KALOGEROPOULOS ◽  
G. N. ANTONAKOPOULOS ◽  
M. B. AGAPITOS ◽  
N. X. PAPACHARALAMPOUS
Keyword(s):  
Cell Carcinoma ◽  
Spindle Cell ◽  
Spindle Cell Carcinoma ◽  
Immunocytochemical Study ◽  
Electron Microscopic

scholarly journals Cytoskeletal reorganization of human platelets after stimulation revealed by the quick-freeze deep-etch technique.

1987 ◽  
Vol 105 (4) ◽  
pp. 1771-1780 ◽  
Author(s):  
T Nakata ◽  
N Hirokawa
Keyword(s):  
Actin Filaments ◽  
Plasma Membranes ◽  
Human Platelets ◽  
Electron Microscopic Level ◽  
Immunocytochemical Study ◽  
Cytoskeletal Reorganization ◽  
Electron Microscopic ◽  
Activated Platelets ◽  
Mixed Polarity ◽  
Cross Bridges

We studied the cytoskeletal reorganization of saponized human platelets after stimulation by using the quick-freeze deep-etch technique, and examined the localization of myosin in thrombin-treated platelets by immunocytochemistry at the electron microscopic level. In unstimulated saponized platelets we observed cross-bridges between: adjoining microtubules, adjoining actin filaments, microtubules and actin filaments, and actin filaments and plasma membranes. After activation with 1 U/ml thrombin for 3 min, massive arrays of actin filaments with mixed polarity were found in the cytoplasm. Two types of cross-bridges between actin filaments were observed: short cross-bridges (11 +/- 2 nm), just like those observed in the resting platelets, and longer ones (22 +/- 3 nm). Actin filaments were linked with the plasma membrane via fine short filaments and sometimes ended on the membrane. Actin filaments and microtubules frequently ran close to the membrane organelles. We also found that actin filaments were associated by end-on attachments with some organelles. Decoration with subfragment 1 of myosin revealed that all the actin filaments associated end-on with the membrane pointed away in their polarity. Immunocytochemical study revealed that myosin was present in the saponin-extracted cytoskeleton after activation and that myosin was localized on the filamentous network. The results suggest that myosin forms a gel with actin filaments in activated platelets. Close associations between actin filaments and organelles in activated platelets suggests that contraction of this actomyosin gel could bring about the observed centralization of organelles.

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