VERO cells (cercopithecus aethiops kidney) ? growth characteristics and viral susceptibility for use in diagnostic virology

1969 ◽  
Vol 27 (2-4) ◽  
pp. 379-385 ◽  
Author(s):  
D. E. Macfarlane ◽  
R. G. Sommerville
Keyword(s):  
Vero Cells ◽  
Growth Characteristics ◽  
Cercopithecus Aethiops ◽  
Viral Susceptibility ◽  
Kidney Growth ◽  
Diagnostic Virology

Comparative growth characteristics of vero cells on gas-permeable and conventional supports

1974 ◽  
Vol 84 (1-2) ◽  
pp. 271-281 ◽  
Author(s):  
M.D. Jensen ◽  
D.F.H. Wallach ◽  
P.-S. Lin
Keyword(s):  
Vero Cells ◽  
Growth Characteristics

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

A combined pseudoreplica-immunocytochemical technique for research and diagnostic virology

1990 ◽  
Vol 48 (3) ◽  
pp. 900-901
Author(s):  
Blayne Fritz ◽  
Stanley J. Naides ◽  
Kenneth Moore
Keyword(s):  
Phosphotungstic Acid ◽  
Immunogold Labelling ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Clinical Specimens ◽  
Tissue Sections ◽  
Specimen Retrieval ◽  
Transmission Electron ◽  
Viral Particles ◽  
Diagnostic Virology

The pseudoreplica method of staining viral particles for visualization by transmission electron microscopy is a very popular technique. The ability to concentrate clinical specimens while semi-embedding viral particles makes it especially well suited for morphologic and diagnostic virology. Immunolabelling viral particles with colloidal gold is a technique frequently employed by both research and diagnostic virologists. We have characterized a procedure which provides the advantage of both by modifying and combining pseudoreplica staining and immunogold labelling.Modification of specimen retrieval and delay of staining allows us to utilize pseudoreplica processed specimens within our standard immunogold labelling protocol. In brief, we absorbed samples onto 2% agarose, added.25% Formvar and wicked dry. We then floated the Formvar-virus film onto double distilled water, added grids and retrieved with parafilm. The Formvar-virus specimens were then treated as thin tissue sections within our standard two stage immunolabelling protocol. Following completion of immunogold labelling; each grid was negatively stained with phosphotungstic acid or uranyl acetate contrast stains.

1123: Natural History and Growth Characteristics of Oncocytomas Under Active Surveillance

2006 ◽  
Vol 175 (4S) ◽  
pp. 361-361
Author(s):  
Paul L. Crispen ◽  
Rosalia Viterbo ◽  
Richard E. Greenberg ◽  
David Y.T. Chen ◽  
Robert G. Uzzo
Keyword(s):  
Natural History ◽  
Active Surveillance ◽  
Growth Characteristics

Comparison of growth characteristics and quality of Korean ginseng (Panax ginseng C.A. Meyer) using different shade materials

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HH Kim ◽  
BJ Seong ◽  
KS Lee ◽  
SI Kim ◽  
JM Geun ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Growth Characteristics ◽  
Korean Ginseng

Cultivation environment under rain shelter and growth characteristics of 5-year old ginseng

2019 ◽  
Author(s):  
YN An ◽  
JA Han ◽  
ES Yi ◽  
GH Park ◽  
HJ An ◽  
...  
Keyword(s):  
Growth Characteristics ◽  
Cultivation Environment

Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aureus and Normal Vero Cells

2019 ◽  
Vol 22 (10) ◽  
pp. 184-190
Author(s):  
Rasha Hadi Saleh ◽  
Entisar J. Al-Mukhtar ◽  
Zaytoon A. Al-Khafaji ◽  
Mohammed H. Al Hasnawy ◽  
Huda H. Al-Hasnawy
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Vero Cells ◽  
Cytotoxic Effects
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