Antioxidant enzyme activities and oxidative stress in human breast cancer

1994 ◽  
Vol 120 (6) ◽  
pp. 374-377 ◽  
Author(s):  
K. Punnonen ◽  
M. Ahotupa ◽  
K. Asaishi ◽  
M. Hy�ty ◽  
R. Kudo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Human Breast Cancer ◽  
Antioxidant Enzyme Activities ◽  
Human Breast ◽  
And Oxidative Stress

Apigenin, a dietary flavonoid, induces apoptosis, DNA damage, and oxidative stress in human breast cancer MCF-7 and MDA MB-231 cells

2018 ◽  
Vol 391 (5) ◽  
pp. 537-550 ◽  
Author(s):  
Ivana Vrhovac Madunić ◽  
Josip Madunić ◽  
Maja Antunović ◽  
Mladen Paradžik ◽  
Vera Garaj-Vrhovac ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Dna Damage ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Dietary Flavonoid ◽  
And Oxidative Stress ◽  
Mcf 7

Antioxidants and oxidative stress in patients with Li-Fraumeni-like syndrome and TP53 p.R337H mutation carriers.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e21042-e21042
Author(s):  
Gabriel de Souza Macedo ◽  
Juliana Giacomazzi ◽  
Cristina Netto ◽  
Leonardo Motta ◽  
Fábio Klamt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Enzyme Activities ◽  
Carbonyl Content ◽  
Oxidative Stress Parameters ◽  
Mutation Carriers ◽  
Li Fraumeni ◽  
And Oxidative Stress ◽  
Stress Parameters

e21042 Background: Germline mutations in the TP53 gene are the underlying genetic defect of Li-Fraumeni Syndrome (LFS) and its variant, Li-Fraumeni-like (LFL) Syndrome, autosomal dominant disorders characterized by predisposition to multiple early-onset cancers. More recently, p53 is emerging as an important player in redox metabolism and important enzymes involved in defenses against oxidative stress have shown to be regulated by p53, including glutathione peroxidase 1 (GPX1) and mitochondrial superoxide dismutase 2 (SOD2). The aim of the present study was to investigate the redox profile parameters in blood of p.R337H mutation carriers and non-carriers individuals. Methods: A total of 34 individuals were included in the study and they were divided in two groups: mutation carriers (n=17) and non-carriers (n=17). Antioxidant enzyme activities (SOD, CAT, GPx) and oxidative stress parameters (Protein carbonyl content, Sulfhydryl content and TBARS) were measured in plasma, erythrocytes, leukocytes and serum. Results: Erythrocyte SOD and GPx activities, two first-line players in enzimatic antioxidant defense, differed significantly between mutation carriers and non-carriers, with an increased activity in the latter (p>0,05). Plasma Carbonyl content, an indicative of protein damage related to ROS overgeneration, was also increased in carriers (p=0,015). There was no significant difference between groups in all other parameters evaluated. Conclusions: Our findings suggest that TP53 p.R337H mutation carriers present different antioxidant enzyme activities and oxidative stress parameters when compared to non-carriers.

scholarly journals In Vitro Modulation of Endogenous Antioxidant Enzyme Activities and Oxidative Stress in Autism Lymphoblastoid Cell Line (ALCL) by Stingless Bee Honey Treatment

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Nazatul Shima Nayan ◽  
Muhammad Aiman Mohd Yazid ◽  
Kamachisree Nallappan ◽  
Amirul Asyiq Amran ◽  
Nur Syuhaidah Zaidi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Antioxidant Enzyme ◽  
Cell Line ◽  
Enzyme Activities ◽  
Lymphoblastoid Cell Line ◽  
Stingless Bee ◽  
Antioxidant Enzyme Activities ◽  
Significant Difference ◽  
And Oxidative Stress

Autism has been associated with a low antioxidant defense mechanism, while honey has been known for decades for its antioxidant and healing properties. Determination of stingless bee honey (KH) effects on antioxidant enzyme activities and oxidative damage in Autism Lymphoblastoid Cell Line (ALCL) was performed. ALCL and its normal sibling pair (NALCL) were cultured in RPMI-1640 medium at 37°C and 5% CO2. ALCL was treated with 400 μg/mL KH (24 h), and oxidative stress marker, malondialdehyde (MDA), and antioxidant enzyme activities (catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)) were measured via enzyme-linked immunosorbent assay (ELISA), while deoxyribonucleic acid (DNA) damage was determined via comet assay. Low SOD activity ( p < 0.05 ) and high MDA level ( p < 0.05 ) were observed in ALCL compared to NALCL. Higher grade (Grades 2 and 3) of DNA damage was highly observed ( p < 0.05 ) in ALCL compared to NALCL, whereas lower grade (Grades 0 and 1) DNA damage was highly detected ( p < 0.05 ) in NALCL compared to ALCL. KH treatment caused a significant increase in SOD and GPx activities ( p < 0.05 ) in ALCL compared to untreated ALCL. Correspondingly, KH treatment reduced the Grade 2 DNA damage ( p < 0.05 ) in ALCL compared to untreated ALCL. CAT activity showed no significant difference between all three groups, while the MDA level showed no significant difference between treated and untreated ALCL. In conclusion, KH treatment significantly reduced the oxidative stress in ALCL by increasing the SOD and GPx antioxidant enzyme activities, while reducing the DNA damage.

Antioxidant enzyme activities and oxidative stress in affective disorders

2004 ◽  
Vol 19 (2) ◽  
pp. 89-95 ◽  
Author(s):  
M. Erkan Ozcan ◽  
Mukaddes Gulec ◽  
Elif Ozerol ◽  
Ruhcan Polat ◽  
Omer Akyol
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Affective Disorders ◽  
Antioxidant Enzyme Activities ◽  
And Oxidative Stress
Sign in / Sign up

Export Citation Format

Share Document