Electron microscopic visualization of intranuclear virus-like bodies in epithelial cells infected with poliomyelitis virus

1956 ◽  
Vol 6 (5) ◽  
pp. 379-387 ◽  
Author(s):  
Helmut Ruska ◽  
Donald C. Stuart ◽  
Johan Winsser
Keyword(s):  
Epithelial Cells ◽  
Electron Microscopic ◽  
Poliomyelitis Virus

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

7191 Electron microscopic study of association between helicobacter pylori and gsatric epithelial cells.

2000 ◽  
Vol 51 (4) ◽  
pp. AB290
Author(s):  
DongKyu Park ◽  
HoonJae Chun ◽  
ByungWon Hur ◽  
ChangDon Kang ◽  
JungWhan Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic

Mise en évidence du rôle, dans la régénération des Amphibiens, d'une glycoprotéine sécrétée par la cape apicale: étude cytochimique et autoradiographique en microscopie électronique

Development ◽  
1974 ◽  
Vol 32 (1) ◽  
pp. 133-145
Author(s):  
Par Claude Chapron
Keyword(s):  
High Resolution ◽  
Epithelial Cells ◽  
Limb Regeneration ◽  
Target Cells ◽  
Silver Particles ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
High Resolution Autoradiography

Evidence for the role of an apical cap glycoprotein in amphibian regeneration: cytochemical and autoradiographic electron-microscopic studies Early during limb regeneration in the newt, an ectodermal apical cap covering a mesodermal blastema is formed. High-resolution autoradiography of these tissues has been carried out after incorporation of [3H]fucose, which is a precursor of glycoproteins. Autoradiography shows that silver particles are located at first on epithelial cells, then on mesenchymatous cells. This observation is consistent with a hypothesis in which the apical cap would elaborate a glycoprotein acting on the blastema. Substructural autoradiography and cytochemistry also show the importance of cellular surfaces for both cells producing glycoprotein and those which are target cells.

The binding of Pseudomonas aeruginosa outer membrane ghosts to human buccal epithelial cells

1986 ◽  
Vol 32 (2) ◽  
pp. 160-166 ◽  
Author(s):  
P. Doig ◽  
A. L. Franklin ◽  
R. T. Irvin
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Outer Membrane ◽  
Copy Number ◽  
Electron Microscopic Examination ◽  
Equilibrium Analysis ◽  
Metabolic Effects ◽  
Electron Microscopic ◽  
Buccal Epithelial Cells ◽  
Number Class

The binding of outer membrane (OM) ghosts derived from Pseudomonas aeruginosa strain 492c to human buccal epithelial cells (BECs) was examined. Electron microscopic examination of the binding of OM ghosts to BECs revealed direct OM ghost–BEC interaction. Equilibrium analysis of the binding of OM ghosts to trypsinized BECs employing the Langmuir adsorption isotherm indicated the number of binding sites (iV) to be 1.3 × 10−1 μg protein per BEC with an apparent association constant (Ka) of 3.4 × 10−2 mL/μg protein. The Langmuir analysis of binding of OM ghosts to untrypsinized BECs was complex, suggesting two possible classes of receptors, a high affinity–low copy number class (Ka, 1.8 × 10−2 mL/μg protein; N, 8.6 × 10−5 μg protein per BEC) and a low affinity – high copy number class(Afa, 3.7 × 10−3 mL/μg protein; N, 9.2 × 10−4 μg protein per BEC). Sugar inhibition studies incorporating D-galactose enhanced binding to each BEC type. N-Acetylneuraminic acid and N-acetyl-glucosamine both enhanced binding of OM ghosts to untrypsinized BECs, while inhibiting binding to trypsinized BECs. D-Arabinose inhibited binding to both BEC types. Binding of OM ghosts to both BEC types was greatly inhibited by D-fucose, while L-fucose only greatly inhibited binding to untrypsinized BECs. These sugar inhibition data demonstrated a difference in the binding of OM ghosts to trypsinized and untrypsinized BECs and possibly reveal the nature of the receptor(s), free of possible bacterial metabolic effects. These data indicated that OM ghosts from 492c appear to bind to BECs in a similar manner to the intact bacteria and represent a simple model system to study the adhesion of P. aeruginosa to BECs.

Electron Microscopic Study of Penetration of Newcastle Disease Virus into Cells Leading to Formation of Polykaryocytes

1967 ◽  
Vol 2 (1) ◽  
pp. 71-76
Author(s):  
N. MEISELMAN ◽  
A. KOHN ◽  
D. DANON
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Newcastle Disease Virus ◽  
Microscopic Study ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cell Membranes ◽  
Viral Envelope ◽  
Electron Microscopic ◽  
Input Multiplicity

Treatment of FL or Lu 106 epithelial cells with Newcastle disease virus (NDV) at an input multiplicity of 500 EID50 per cell induces in these cells the formation of polykaryocytes at the end of 2-3 h of contact. Electron micrographs of such NDV-treated monolayers after 2-10 min of incubation show the presence of virions adsorbed to the cell membranes, in vacuoles and with the viral envelope partly fused with the cell membrane. In polykaryocytes induced by NDV, remnants of cell membranes showing numerous breaks may still be present after 3 h.

scholarly journals Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells.

1985 ◽  
Vol 100 (1) ◽  
pp. 327-332 ◽  
Author(s):  
K Boller ◽  
D Vestweber ◽  
R Kemler
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Intestinal Epithelial Cells ◽  
Rabbit Antiserum ◽  
Preimplantation Embryos ◽  
Intestinal Epithelial ◽  
Electron Microscopic ◽  
A Cell

Uvomorulin is a cell-adhesion molecule implicated in the compaction process of mouse preimplantation embryos and the aggregation of embryonal carcinoma cells. A rabbit antiserum against purified uvomorulin also reacts with epithelial cells of various adult tissues. In this study, we investigated the localization of uvomorulin on adult intestinal epithelial cells using electron microscopic analyses. Uvomorulin was shown to exhibit a highly restricted localization in the intermediate junctions of these cells. The results are discussed with respect to a possible adhesive function of uvomorulin on intestinal epithelial cells.

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