A new approach for development of rugged sample preparation of metabolites of albendazole in cow milk

1999 ◽  
Vol 130 (3) ◽  
pp. 155-163 ◽  
Author(s):  
Zsuzsanna Romv�ri ◽  
S�ndor Kem�ny ◽  
Gy�rgy Pokol ◽  
Jen� Fekete
Keyword(s):  
Sample Preparation ◽  
Cow Milk ◽  
New Approach

scholarly journals Evaluation of sample preparation methods for NMR-based metabolomics of cow milk

Heliyon ◽  
2018 ◽  
Vol 4 (10) ◽  
pp. e00856 ◽  
Author(s):  
Bénédict Yanibada ◽  
Hamid Boudra ◽  
Laurent Debrauwer ◽  
Cécile Martin ◽  
Diego P. Morgavi ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Cow Milk ◽  
Preparation Methods ◽  
Sample Preparation Methods

scholarly journals Effect of Fat on Protein Estimation in Milk and Its Correlation with Lactose in Different Milk Types: A Small-Scale Study

2020 ◽  
Author(s):  
Sweekruthi A. Shetty ◽  
Melissa F. Young ◽  
Sunita Taneja ◽  
Kannan Rangiah
Keyword(s):  
Sample Preparation ◽  
Human Milk ◽  
Internal Standard ◽  
Cow Milk ◽  
Selected Reaction Monitoring ◽  
Small Scale ◽  
Milk Samples ◽  
Protein Estimation ◽  
Animal Milk ◽  
Fat Removal

Background: Estimation of macronutrients like protein and lactose is important to assess the quality of milk. To estimate these two macronutrients, ten raw milk samples obtained from each group of different animals (cow, goat, buffalo), ten pasteurized cow milk and ten human milk samples were analysed. Methods: Bicinchoninic acid (BCA) method was used to estimate protein from different milk samples. Four different sample preparation protocols were compared to check the effect of fat on BCA based protein estimation: dilution (D), fat removal-protein precipitation (FR and PP), fat removal-dilution (FR and D) and dilution-fat removal (D and FR). For lactose quantification, ultrahigh-performance liquid chromatography-mass spectrometry-selected reaction monitoring (UHPLC-MS/SRM) method was developed and validated using 13C6 lactose as internal standard (ISTD).Result: Among these four different protocols, D and FR method showed consistent data for total protein content in animal milk (cow-3.16%, goat-3.21%, buffalo-3.81%, pasteurized-2.98%) and FR and PP showed consistent data in human milk samples (1.2%). Though BCA method is simple to use, proper sample preparation protocol has to be applied prior to protein estimation to avoid the interference due to fat or lactose. In case of lactose, inter-day validation showed the accuracy ranging from 97.13 to 100.54%, coefficient of variation varying between 0.1 to 1.53%, correlation R2=0.999. Lactose is in the range of 4.1 to 4.8% in animal milk and 6.6% in human milk samples. The internal ratio of lactose/protein (1.28 to 1.55 in animal milk and 5.33 in human milk) will be useful to differentiate human milk from animal milk type and to assess the milk quality.

A new approach to the quality control of steroid receptor assays.

1988 ◽  
Vol 34 (10) ◽  
pp. 2076-2079
Author(s):  
M Borsotti ◽  
M Quercioli ◽  
R D'Ettole ◽  
A Piffanelli ◽  
G Messeri
Keyword(s):  
Quality Control ◽  
Sample Preparation ◽  
Steroid Receptors ◽  
Menopausal Status ◽  
Steroid Receptor ◽  
Usual Practice ◽  
New Approach ◽  
Breast Tumor Tissue ◽  
Consensus Mean ◽  
Receptor Assays

Abstract Lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. However, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. Participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). After verifying the homogeneity of the populations investigated, we computed consensus means for the percentages of positive samples and for their absolute value. Despite the homogeneity of the characteristics of the populations studied, results from some laboratories systematically differed from the consensus mean. This approach to the quality control of steroid receptors allows evaluation of the whole procedure, from sample preparation to analysis, and thus may be helpful as an addition to the usual practice of distribution of cytosols and tissue powders for assay.

scholarly journals A new approach to mixing techniques for enhanced performance in automated sample preparation

1995 ◽  
Vol 17 (5) ◽  
pp. 169-171
Author(s):  
François Qian ◽  
Eric Vérette ◽  
Atika El-Sayed
Keyword(s):  
Sample Preparation ◽  
Glucose Solution ◽  
New Approach ◽  
Relative Standard ◽  
Different Types ◽  
Mixing Method ◽  
Automated Sample Preparation ◽  
Standard Deviations ◽  
Mixing Techniques ◽  
Sample Dilution

In the automation of sample dilution or derivatization, the performance of the mixing technique employed when adding solvents or reagents to samples is critical. This paper presents a newly developed mixing method, based on conventional aspiration and dispensing of liquid techniques, but which considerably improves the precision of mixing. The paper discusses the results of a comparison of the technique with other methods and describes the application of the technique to several different types of sample solutions, including a highly concentrated glucose solution. The mixing technique was performed on a Gilson XL Sampling Injector, with a 1/25 dilution of a paraben solution in 2 ml vials to give relative standard deviations of 0.2 to 0.3% (N =10).

scholarly journals Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2086 ◽  
Author(s):  
Martha Maggira ◽  
Eleni A. Deliyanni ◽  
Victoria F. Samanidou
Keyword(s):  
Graphene Oxide ◽  
Sample Preparation ◽  
Cow Milk ◽  
Preparation Technique ◽  
Limit Of Quantification ◽  
Sorbent Material ◽  
Novel Material ◽  
Polyurethane Sponge ◽  
Fast Procedure

In the present study, a novel, simple, and fast sample preparation technique is described for the determination of four sulfonamides (SAs), namely Sulfathiazole (STZ), sulfamethizole (SMT), sulfadiazine (SDZ), and sulfanilamide (SN) in cow milk prior to HPLC. This method takes advantage of a novel material that combines the extractive properties of graphene oxide (GO) and the known properties of common polyurethane sponge (PU) and that makes sample preparation easy, fast, cheap and efficient. The PU-GO sponge was prepared by an easy and fast procedure and was characterized with FTIR spectroscopy. After the preparation of the sorbent material, a specific extraction protocol was optimized and combined with HPLC-UV determination could be applied for the sensitive analysis of trace SAs in milk. The proposed method showed good linearity while the coefficients of determination (R2) were found to be high (0.991–0.998). Accuracy observed was within the range 90.2–112.1% and precision was less than 12.5%. Limit of quantification for all analytes in milk was 50 μg kg−1. Furthermore, the PU-GO sponge as sorbent material offered a very clean extract, since no matrix effect was observed.

scholarly journals Sample Preparation Techniques for the Analysis of Microplastics in Soil—A Review

2020 ◽  
Vol 12 (21) ◽  
pp. 9074 ◽  
Author(s):  
Daniela Thomas ◽  
Berit Schütze ◽  
Wiebke Mareile Heinze ◽  
Zacharias Steinmetz
Keyword(s):  
Sample Preparation ◽  
Best Practice ◽  
Method Development ◽  
Complex Nature ◽  
Fenton Reagent ◽  
Plastic Pollution ◽  
New Approach ◽  
Practice Methods ◽  
Preparation Techniques ◽  
Soil Constituents

Although most plastic pollution originates on land, current research largely remains focused on aquatic ecosystems. Studies pioneering terrestrial microplastic research have adapted analytical methods from aquatic research without acknowledging the complex nature of soil. Meanwhile, novel methods have been developed and further refined. However, methodical inconsistencies still challenge a comprehensive understanding of microplastic occurrence and fate in and on soil. This review aims to disentangle the variety of state-of-the-art sample preparation techniques for heterogeneous solid matrices to identify and discuss best-practice methods for soil-focused microplastic analyses. We show that soil sampling, homogenization, and aggregate dispersion are often neglected or incompletely documented. Microplastic preconcentration is typically performed by separating inorganic soil constituents with high-density salt solutions. Not yet standardized but currently most used separation setups involve overflowing beakers to retrieve supernatant plastics, although closed-design separation funnels probably reduce the risk of contamination. Fenton reagent may be particularly useful to digest soil organic matter if suspected to interfere with subsequent microplastic quantification. A promising new approach is extraction of target polymers with organic solvents. However, insufficiently characterized soils still impede an informed decision on optimal sample preparation. Further research and method development thus requires thorough validation and quality control with well-characterized matrices to enable robust routine analyses for terrestrial microplastics.

Extraction of estrogen hormones from water samples using an aqueous two-phase system: a new approach for sample preparation in the analysis of emerging contaminants

2021 ◽  
pp. 106231
Author(s):  
Junielly Tomaz Domingues ◽  
Ricardo Mathias Orlando ◽  
Mariana Ramos Almeida ◽  
Leandro Rodrigues de Lemos ◽  
Aparecida Barbosa Mageste ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Water Samples ◽  
Emerging Contaminants ◽  
Phase System ◽  
Two Phase ◽  
New Approach ◽  
Aqueous Two Phase System ◽  
System A
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