Copper(II)-neocuproine as colour reagent for some biologically active thiols: Spectrophotometric determination of cysteine, penicillamine, glutathione, and 6-mercaptopurine

1989 ◽  
Vol 99 (1-2) ◽  
pp. 143-146 ◽  
Author(s):  
Amir Besada ◽  
Nabil B. Tadros ◽  
Yousseff A. Gawargious
Keyword(s):  
Spectrophotometric Determination ◽  
Biologically Active

Flow-Injection Spectrophotometric Determination of Cysteine in Biologically Active Dietary Supplements

2016 ◽  
Vol 71 (2) ◽  
pp. 172-178 ◽  
Author(s):  
A. V. Petrova ◽  
R. Ishimatsu ◽  
K. Nakano ◽  
T. Imato ◽  
A. B. Vishnikin ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Spectrophotometric Determination ◽  
Flow Injection ◽  
Biologically Active

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF ANTIOXIDANT SODIUM METABISULPHITE CONTENT IN PREPARED MEDICINAL FORMS OF VETERINARY MEDICINAL PRODUCTS

2020 ◽  
Vol 21 (2) ◽  
pp. 189-198
Author(s):  
H. Yu. Teslyar ◽  
M. Ya. Smolinska ◽  
I. Ya. Kotsyumbas ◽  
N. M. Chyhyn ◽  
N. G. Rohulia ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
Storage Time ◽  
Analytical Signal ◽  
Veterinary Drugs ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Optimal Conditions ◽  
Sodium Metabisulphite ◽  
Active Substances

An analytical method for the quantitative determination of antioxidant sodium metabisulphite in veterinary drugs has been proposed by spectrophotometric method. Based on the literature data, the optimal conditions of the analytical reaction were selected experimentally. The dependence of the value of the analytical signal on the temperature of the reaction medium, concentration of p-rosaniline and duration of the reaction was investigated to establish the optimal conditions of the analytical reaction and obtain a stable analytical signal. The stability of the colored analytical form in time was also checked and the linear dependence of the value of the analytical signal on the concentration of sodium metabisulphite was investigated. The analytical reaction at room temperature is optimal. The maximum analaytical signal is achieved by carrying out the analytical reaction for 10 minutes and then practically does not change for an hour. To achieve the maximum analytical signal, it is necessary to use a 20-fold excess of dye relative to sodium metabisulphite. The analytical signal remains stable only for the first hour, then gradually begins to decrease. Metrological characteristics of the method of determination of metabisulphite in veterinary drugs are calculated, the limits of spectrophotometric determination are 0.33 – 2.50 μg/ml. The correctness of the developed method was checked on model solutions by the method of "introduced-found" method of comparison in the presence of various biologically active substances that are part of drugs together with sodium metabisulphite. The content of sodium metabisulphite in veterinary drugs of domestic and foreign production at different intervals of their storage time was established. It is shown that the content of sodium metabisulphite in drugs decreases during their storage time, until complete disappearance, which directly affects the content of the active substance, because in the absence of antioxidant oxidative processes with biologically active substances begin to take place.

scholarly journals Spectrophotometric Determination of Biologically Active Thiols with Eosin, Silver(I) and Adenine.

2002 ◽  
Vol 18 (9) ◽  
pp. 981-985 ◽  
Author(s):  
Yoshikazu FUJITA ◽  
Itsuo MORI ◽  
Takako YAMAGUCHI
Keyword(s):  
Spectrophotometric Determination ◽  
Biologically Active

scholarly journals The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material

2021 ◽  
pp. 17-26
Author(s):  
V.V. Velichko ◽  
◽  
D.S. Kruglov ◽  
Keyword(s):  
Spectrophotometric Determination ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Health Improvement ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Wide Range ◽  
Vitamin Activity

Carotenoids are of interest as precursors of vitamin A, which has a wide range of pharmacological effects — antioxidant, immunomodulatory, participating in photoreception, etc. The aim of the research was to develop a technique for the spectrophotometric determination of the A-vitamin activity of carotenoids-rich medicinal plant raw materials (MPRM). The objects of research were the traditional sources of carotenoids — sea buckthorn, carrot, pumpkin and wild rose. A method is proposed for determining the content of individual carotenoids in MPRM by optical density at wavelengths corresponding to the characteristic peaks of α-carotene, β-carotene and lycopene. It has been shown that it is preferable to use isopropyl alcohol as the most effective solvent. Taking into account the content of specific carotenoids makes it possible to determine the A-vitamin activity of the sum of carotenoids extracted from plant materials, and, accordingly, to increase the efficiency of the use of herbal preparations and biologically active additives for health improvement.

Stepwise injection spectrophotometric determination of cysteine in biologically active supplements and fodders

2013 ◽  
Vol 110 ◽  
pp. 369-373 ◽  
Author(s):  
Andrey V. Bulatov ◽  
Anastasiia V. Petrova ◽  
Andriy B. Vishnikin ◽  
Leonid N. Moskvin
Keyword(s):  
Spectrophotometric Determination ◽  
Biologically Active

Spectrophotometric Determination of Total Cr in Water

2018 ◽  
Author(s):  
Xhulieta Hamiti ◽  
Ilirjana Boci ◽  
Pranvera Lazo
Keyword(s):  
Spectrophotometric Determination
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