Mouse leukaemia, a dichotomy of virus neutralizing and cytotoxic antibodies

1966 ◽  
Vol 19 (1) ◽  
pp. 75-80 ◽  
Author(s):  
J. G. Sinkovics ◽  
C. C. Shullenberger ◽  
C. D. Howe ◽  
Barbara A. Bertin
Keyword(s):  
Cytotoxic Antibodies ◽  
Mouse Leukaemia

scholarly journals Mouse and human ornithine decarboxylase genes. Methylation polymorphism and amplification

1987 ◽  
Vol 242 (1) ◽  
pp. 205-210 ◽  
Author(s):  
L Alhonen-Hongisto ◽  
P Leinonen ◽  
R Sinervirta ◽  
R Laine ◽  
R Winqvist ◽  
...  
Keyword(s):  
Cell Line ◽  
Ornithine Decarboxylase ◽  
Ehrlich Ascites Carcinoma ◽  
Myeloma Cell ◽  
Restriction Pattern ◽  
Restriction Endonucleases ◽  
Cell Leukaemia ◽  
Chromosome 2 ◽  
Ehrlich Ascites ◽  
Mouse Leukaemia

With the use of the isoschizomeric restriction endonucleases HpaII and MspI, we found that mouse tumour ornithine decarboxylase (ODC; EC 4.1.1.17) genes are extensively methylated. ODC genes in L1210 mouse leukaemia cells were apparently more methylated than in Ehrlich ascites carcinoma, as revealed by the use of HpaII endonuclease, yet the digestion of genomic DNA isolated from these two murine tumour cell lines with MspI, which cleaves at a CCGG sequence, also with internally methylated cytosine, resulted in an apparently identical restriction pattern. It is possible that the amplification of ODC genes in Ehrlich ascites-carcinoma cells in response to 2-difluoromethylornithine (DFMO) was associated with hypomethylation, or that less-methylated genes were amplified. A human myeloma (Sultan) cell line only revealed three separate hybridization signals when cleaved with HpaII. One of these signals was amplified under the pressure of DFMO. When cleaved with MspI, these three HpaII fragments disappeared and were replaced by a double signal of 2.3-2.4 kilobase-pairs (kbp) in size. The amplified ODC sequences in the Sultan myeloma cell line apparently originated from chromosome 2, as indicated by a unique hybridization signal in a 5.8 kbp HindIII fragment specific for the human ODC locus on chromosome 2. A comparison of different human cells, the Sultan myeloma, a lymphocytic B-cell leukaemia (Ball), normal mononuclear leucocytes and leucocytes obtained from leukaemia patients, revealed interesting differences in the methylation of ODC genes. The use of two restriction endonucleases (HpaII and CfoI), the cleavage site for both of which contains a CG sequence and which only cleave when cytosine is unmethylated, indicated that ODC genes in the lymphocytic leukaemia cells were much less methylated than those in the normal leucocytes or in the Sultan cells.

CYTOTOXIC ANTIBODIES IN SERUM OF PREGNANT WOMEN AT DELIVERY

2009 ◽  
Vol 79B (4) ◽  
pp. 595-598
Author(s):  
Gunnar Nymand ◽  
Iver Heron ◽  
Kaj Gert Jensen ◽  
Anne Lundsgaard
Keyword(s):  
Pregnant Women ◽  
Cytotoxic Antibodies

Effects of prostaglandins E1, E2, and F2alpha on the growth of leukaemia cells in culture

1976 ◽  
Vol 20 (1) ◽  
pp. 199-206
Author(s):  
T.J. Yang ◽  
J.B. Dale ◽  
R. Machanoff
Keyword(s):  
Tritiated Thymidine ◽  
Inhibitory Effect ◽  
Soft Agar ◽  
Significant Inhibition ◽  
Leucine Incorporation ◽  
Inhibitory Effects ◽  
Uridine Incorporation ◽  
Cells In Culture ◽  
Mouse Leukaemia

Prostaglandins E1, E2, and F2alpha (PGE1, PGE2, and PGF2alpha) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE1 and PGE2 were greater than that of PGF2alpha. PGE1 and PGE2, at the concentration of 100 mug per ml showed significant inhibitory effects on the rates of incorporation of tritiated thymidine, uridine and leucine. At concentrations of 50 and 25 mug per ml, there was significant inhibition of thymidine and uridine incorporation, but not of leucine, PGF2alpha showed significant inhibition of thymidine and uridine incorporation but not leucine incorporation, in all 3 concentrations studied (100, 50, and 25 mug/ml). The ability of the cells to form colonies in soft agar was significantly inhibited by PGE1 and PGE2 at concentrations as low as 1–8 mug/ml. For F2alpha, however, a concentration as high as 56mug/ml was required to show inhibitory effect, but at 1–8 mug/ml it was found to be stimulatory.

280: Low Likelihood for Developing Cytotoxic Antibodies during Implantation with the CardioWest Total Artificial Heart

2008 ◽  
Vol 27 (2) ◽  
pp. S161 ◽  
Author(s):  
P.E. Nolan ◽  
R.G. Smith ◽  
M.J. Slepian ◽  
R.K. Bose ◽  
P.H. Tsau ◽  
...  
Keyword(s):  
Artificial Heart ◽  
Total Artificial Heart ◽  
Cytotoxic Antibodies

scholarly journals Role of anti-human lymphocyte culture cytotoxic antibodies in recurrent spontaneous pregnancy loss women

2011 ◽  
Vol 4 (1) ◽  
pp. 17 ◽  
Author(s):  
Shankarkumar Umapathy ◽  
Aruna Shankarkumar ◽  
Vanita Ramrakhiyani ◽  
Kanjaksha Ghosh
Keyword(s):  
Human Lymphocyte ◽  
Pregnancy Loss ◽  
Lymphocyte Culture ◽  
Cytotoxic Antibodies ◽  
Spontaneous Pregnancy
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