scholarly journals Precipitin reactions between insulin, proinsulin, insulin chains and insulin antibody

Diabetologia ◽  
1973 ◽  
Vol 9 (5) ◽  
pp. 384-386 ◽  
Author(s):  
D. D. Bansal ◽  
J. H. Connolly ◽  
J. Vallance-Owen
Keyword(s):  
Insulin Antibody ◽  
Insulin Chains

Combination of insulin chains on an anti-insulin antibody–Sepharose column

Nature ◽  
1976 ◽  
Vol 262 (5571) ◽  
pp. 791-793 ◽  
Author(s):  
MECHTHILD KLOTZ ◽  
BERND GUTTE
Keyword(s):  
Insulin Antibody ◽  
Sepharose Column ◽  
Insulin Chains

Sites of Insulin Action Using Ferritin Labeling

1971 ◽  
Vol 29 ◽  
pp. 540-541
Author(s):  
Burton B. Silver ◽  
Ronald S. Nelson
Keyword(s):  
Electron Microscopy ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Insulin Antibody ◽  
Fat Pad ◽  
Target Organs ◽  
Uranium Salts ◽  
Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

PLACENTAL TRANSFER OF INSULIN-131I IN GUINEA PIGS IMMUNIZED AGAINST INSULIN

1966 ◽  
Vol 52 (2) ◽  
pp. 276-291 ◽  
Author(s):  
Jan I. Thorell
Keyword(s):  
Plasma Concentration ◽  
Guinea Pigs ◽  
Placental Transfer ◽  
Maternal Plasma ◽  
Insulin Antibodies ◽  
Insulin Antibody

ABSTRACT The placenta is considered to be impermeable or only slightly permeable to insulin. Insulin antibodies are transferred from mother to foetus in man and in guinea pigs. The passage of insulin-131I from mother to foetus was studied in guinea pigs with and without antibodies against insulin. Antibody-bound insulin-131I was recovered in plasma from foetuses of immunized pregnant guinea pigs, at intervals of more than 5 hours after the injection of insulin-131I to the mother. The foetal levels of insulin-131I were rather low, the highest recorded value being 27% of the maternal plasma concentration. This peak was reached 32 hours after the injection. No insulin-131I was found in the foetuses of non-immunized guinea pigs.

Relation between insulin antibody and complement-fixing islet cell antibody at clinical diagnosis of IDDM

Diabetes ◽  
1986 ◽  
Vol 35 (5) ◽  
pp. 620-622 ◽  
Author(s):  
J. Karjalainen ◽  
M. Knip ◽  
A. Mustonen ◽  
J. Ilonen ◽  
H. K. Akerblom
Keyword(s):  
Clinical Diagnosis ◽  
Islet Cell ◽  
Islet Cell Antibody ◽  
Insulin Antibody ◽  
Cell Antibody

Isoelectric focusing of insulin, 125I-insulin and the 125I-insulin-antibody complex

1979 ◽  
Vol 44 (6) ◽  
pp. 1828-1834
Author(s):  
Asja Šiševa ◽  
Jiřina Slaninová ◽  
Tomislav Barth ◽  
Stephan P. Ditzov ◽  
Luben M. Sirakov
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
The Other ◽  
Insulin Antibody ◽  
Isoelectric Points ◽  
Antibody Complex ◽  
And Storage ◽  
Acid Region ◽  
Three Components

Isoelectric focusing on polyacrylamide gel columns of three native crystalline commercial preparations of insulin and 125I-labelled insulin was carried out. All the compounds studied contained three components of different isoelectric points. The largest fraction, having pI 5.60 ± 0.05, was common to all preparations. The other two fractions were situated in the acid region of pH between pI 4.5 and 5.2. The presence of these fractions is explained by the contamination of crystalline insulins by proinsulin and by the formation of des-amido derivatives during the dissolving and storage of insulin samples, and, in case of labelled insulin, also by the presence of heavily iodinated insulin and contaminating components. The isoelectric focusing of the complex 125I-insulin-antibody showed a peak of radioactivity having pI 6.15 ± 0.05.

scholarly journals Clinical Outcomes of Patients with Diabetes Who Exhibit Upper-Quartile Insulin Antibody Responses After Treatment with LY2963016 or Lantus® Insulin Glargine

2017 ◽  
Vol 8 (3) ◽  
pp. 545-554 ◽  
Author(s):  
Liza L. Ilag ◽  
Timothy M. Costigan ◽  
Mark A. Deeg ◽  
Robyn K. Pollom ◽  
Curtis L. Chang ◽  
...  
Keyword(s):  
Insulin Glargine ◽  
Clinical Outcomes ◽  
Antibody Responses ◽  
Insulin Antibody ◽  
Patients With Diabetes

Transforming growth factor-alpha (TGF-alpha) and insulin gene expression in human fetal pancreas

Development ◽  
1992 ◽  
Vol 114 (4) ◽  
pp. 833-840 ◽  
Author(s):  
P.J. Miettinen ◽  
K. Heikinheimo
Keyword(s):  
Growth Factor ◽  
Beta Cells ◽  
Copy Number ◽  
Transforming Growth Factor ◽  
Insulin Antibody ◽  
Transforming Growth Factor Alpha ◽  
Double Labeling ◽  
Fetal Pancreas ◽  
Insulin Mrna ◽  
Factor Alpha

Transforming growth factor-alpha (TGF-alpha) mRNA is expressed in several pancreatic cancer cell lines, but its expression during normal fetal pancreas development has not been studied. We investigated the expression of TGF-alpha, its receptor (EGF-R) and insulin mRNA and their corresponding peptides in human fetal pancreata (15–20 gestation weeks). Polymerase chain reaction (PCR) and RNAase protection analysis revealed that TGF-alpha and insulin mRNAs were detectable in pancreas during the developmental span studied. In northern blot analysis a single band of 4.8 kilobases (kb) corresponding to the TGF-alpha transcript and a 0.6 kb for the insulin mRNA were detected in the pancreas. Using in situ hybridization, TGF-alpha mRNA expression was seen in a low copy number in both the exo- and endocrine pancreas. By immunohistochemistry TGF-alpha-immunoreactive cells were detected in the ducts, acini and islets showing that the mRNA was translated into protein. By contrast, insulin transcripts were detected in a high copy number, restricted to the islets of Langerhans. However, monoclonal insulin antibody detected less insulin containing cells than could be expected from the mRNA pattern suggesting that fetal beta-cells rapidly secrete insulin instead of storing it in the secretory granules. Alternatively, the translation of insulin mRNA could be inefficient. By double labeling the pancreas sections with polyclonal TGF-alpha antiserum and monoclonal insulin antibody the TGF-alpha- and insulin-like immunoreactivity was localized to beta-cells. Furthermore, mRNA for the TGF-alpha receptor, EGF-R, together with EGF-R-immunoreactive cells were also present in pancreas.(ABSTRACT TRUNCATED AT 250 WORDS)

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