Effects of hyaluronidase on doxorubicin penetration into squamous carcinoma multicellular tumor spheroids and its cell lethality

1994 ◽  
Vol 120 (5) ◽  
pp. 293-297 ◽  
Author(s):  
Naoyuki Kohno ◽  
Takao Ohnuma ◽  
Peter Truog
Keyword(s):  
Squamous Carcinoma ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Cell Lethality

Evaluation of Taxol?? in head and neck squamous carcinoma multicellular tumor spheroids

1997 ◽  
Vol 8 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Genevl??ve Griffon-Etienne ◽  
Jean-Louis Merlin ◽  
Christian Marchal
Keyword(s):  
Head And Neck ◽  
Squamous Carcinoma ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids

Spatially resolved metabolomics combined with multicellular tumor spheroids to discover cancer tissue relevant metabolic signatures

2021 ◽  
Vol 1155 ◽  
pp. 338342
Author(s):  
Qingce Zang ◽  
Chenglong Sun ◽  
Xiaoping Chu ◽  
Limei Li ◽  
Wenqiang Gan ◽  
...  
Keyword(s):  
Cancer Tissue ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Spatially Resolved ◽  
Metabolic Signatures

scholarly journals Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karl Olofsson ◽  
Valentina Carannante ◽  
Madoka Takai ◽  
Björn Önfelt ◽  
Martin Wiklund
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Drug Distribution ◽  
In Vitro Models ◽  
Biological Information ◽  
Dimensional Structure ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Nuclear Segmentation

AbstractMulticellular tumor spheroids (MCTSs) can serve as in vitro models for solid tumors and have become widely used in basic cancer research and drug screening applications. The major challenges when studying MCTSs by optical microscopy are imaging and analysis due to light scattering within the 3-dimensional structure. Herein, we used an ultrasound-based MCTS culture platform, where A498 renal carcinoma MCTSs were cultured, DAPI stained, optically cleared and imaged, to connect nuclear segmentation to biological information at the single cell level. We show that DNA-content analysis can be used to classify the cell cycle state as a function of position within the MCTSs. We also used nuclear volumetric characterization to show that cells were more densely organized and perpendicularly aligned to the MCTS radius in MCTSs cultured for 96 h compared to 24 h. The method presented herein can in principle be used with any stochiometric DNA staining protocol and nuclear segmentation strategy. Since it is based on a single counter stain a large part of the fluorescence spectrum is free for other probes, allowing measurements that correlate cell cycle state and nuclear organization with e.g., protein expression or drug distribution within MCTSs.

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