Location of profilin at presynaptic sites in the cerebellar cortex; implication for the regulation of the actin-polymerization state during axonal elongation and synaptogenesis

1993 ◽  
Vol 22 (12) ◽  
pp. 1060-1072 ◽  
Author(s):  
C. Faivre-Sarrailh ◽  
J. Y. Lena ◽  
L. Had ◽  
M. Vignes ◽  
U. Lindberg
Keyword(s):  
Cerebellar Cortex ◽  
Actin Polymerization ◽  
Axonal Elongation ◽  
Actin Polymerization State

Receptor dynamics regulates actin polymerization state through phosphorylation of cofilin in mast cells

2021 ◽  
Vol 534 ◽  
pp. 714-719
Author(s):  
Ruriko Suzuki ◽  
Yoshikazu Inoh ◽  
Satoru Yokawa ◽  
Tadahide Furuno ◽  
Naohide Hirashima
Keyword(s):  
Mast Cells ◽  
Actin Polymerization ◽  
Receptor Dynamics ◽  
Actin Polymerization State

scholarly journals Actin Polymerization State Regulates Osteogenic Differentiation in Human Adipose-derived Stem Cells

2020 ◽  
Author(s):  
Bing Sun ◽  
Xin Jiang ◽  
Rongmei Qu ◽  
Tingyu Fan ◽  
Yuchao Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Osteogenic Differentiation ◽  
Focal Adhesion ◽  
Actin Polymerization ◽  
Dependent Manner ◽  
Adipose Derived Stem Cells ◽  
Alizarin Red ◽  
20 Nm ◽  
Actin Polymerization State

Abstract Background:Actin is an essential cellular protein that assembles into microfilaments and regulates numerous processes such as cell migration, maintenance of cell shape, and material transport. In this study, we explored the effect of actin polymerization state on the osteogenic differentiation of human adipose-derived stem cells (hASCs). Methods:The hASCs were treated with different concentrations (0, 1, 5, 10, 20, and 50 nM)of jasplakinolide (JAS), a reagent that directly polymerizes F-actin.The effects ofthe actin polymerization state on cell proliferation, apoptosis, migration, and the maturity of focal adhesion-related proteins were assessed. In addition, western blotting and alizarin red staining assays were performed to assess osteogenic differentiation. Results: These results revealed that cell proliferation and migration in the JAS (0, 1, 5, 10, and 20 nM) groupswashigher than that inthe control group andthe JAS (50 nM) group.The protein expressionof focal adhesion kinase, vinculin, paxillin, and talinwere highest in the JAS (20 nM) group, whilezyxin expression was highestinthe JAS (50 nM) group.Western blottingshowed thatosteogenic differentiation in theJAS (0, 1, 5, 10, 20, and 50 nM) groupswas enhanced compared with that in thecontrol group, and was strongest inthe JAS (50 nM) group.Conclusions: Our data suggest thatthe actinpolymerization state may promote the osteogenic differentiation of hASCs by regulating the protein expression of focal adhesion-associated proteins in a concentration-dependent manner. Our findings provide valuable information for exploring the mechanism of osteogenic differentiationin hASCs.

scholarly journals Actin polymerization state regulates osteogenic differentiation in human adipose-derived stem cells

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Bing Sun ◽  
Rongmei Qu ◽  
Tingyu Fan ◽  
Yuchao Yang ◽  
Xin Jiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Protein Expression ◽  
Osteogenic Differentiation ◽  
Focal Adhesion ◽  
Actin Polymerization ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
20 Nm ◽  
Actin Polymerization State

Abstract Background Actin is an essential cellular protein that assembles into microfilaments and regulates numerous processes such as cell migration, maintenance of cell shape, and material transport. Methods In this study, we explored the effect of actin polymerization state on the osteogenic differentiation of human adipose-derived stem cells (hASCs). The hASCs were treated for 7 days with different concentrations (0, 1, 5, 10, 20, and 50 nM) of jasplakinolide (JAS), a reagent that directly polymerizes F-actin. The effects of the actin polymerization state on cell proliferation, apoptosis, migration, and the maturity of focal adhesion-related proteins were assessed. In addition, western blotting and alizarin red staining assays were performed to assess osteogenic differentiation. Results Cell proliferation and migration in the JAS (0, 1, 5, 10, and 20 nM) groups were higher than in the control group and the JAS (50 nM) group. The FAK, vinculin, paxillin, and talin protein expression levels were highest in the JAS (20 nM) group, while zyxin expression was highest in the JAS (50 nM) group. Western blotting showed that osteogenic differentiation in the JAS (0, 1, 5, 10, 20, and 50 nM) group was enhanced compared with that in the control group, and was strongest in the JAS (50 nM) group. Conclusions In summary, our data suggest that the actin polymerization state may promote the osteogenic differentiation of hASCs by regulating the protein expression of focal adhesion-associated proteins in a concentration-dependent manner. Our findings provide valuable information for exploring the mechanism of osteogenic differentiation in hASCs.

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