scholarly journals Biochemical and ultrastructural changes in A and B cells of the islets of Langerhans of mice infected with EMC virus

Diabetologia ◽  
1979 ◽  
Vol 17 (1) ◽  
pp. 51-57 ◽  
Author(s):  
F. Zaheer ◽  
S. L. Howell ◽  
K. W. Taylor ◽  
T. J. Coleman ◽  
D. R. Gamble
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Ultrastructural Changes ◽  
Emc Virus ◽  
A And B Cells

scholarly journals CYTOCHEMICAL LOCALIZATION OF ADENYL CYCLASE ACTIVITY IN RAT ISLETS OF LANGERHANS

1972 ◽  
Vol 20 (11) ◽  
pp. 873-879 ◽  
Author(s):  
S. L. HOWELL ◽  
MARGARET WHITFIELD
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Adenosine Triphosphate ◽  
Adenyl Cyclase ◽  
Cyclase Activity ◽  
Cytochemical Localization ◽  
Adenyl Cyclase Activity ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
A And B Cells

A cytochemical method has been used to investigate the localization of adenyl cyclase activity in A and B cells of isolated rat islets of Langerhans. Adenosine triphosphate was initially utilized as substrate, the pyrophosphate liberated being precipitated by lead ions at its site of production. The specificity of the method was increased by the use of adenylyl-imidodiphosphate as an alternative substrate; this adenosine triphosphate analogue was not hydrolyzed by adenosine triphosphatase but provided an effective substrate for adenyl cyclase. Adenyl cyclase activity, which was found to retain its glucagon and fluoride sensitivity in glutaraldehyde-fixed tissue, was found exclusively and almost uniformly in the plasma membranes of A and B cells. Storage granule membrane, incorporated into the plasma membrane during secretion of the granule content by exocytosis, appeared to be devoid of adenyl cyclase activity.

Cryo-Ultramicrotomy of Islets of Langerhans

1974 ◽  
Vol 15 (3) ◽  
pp. 591-603
Author(s):  
S. L. HOWELL ◽  
MARGARET TYHURST
Keyword(s):  
Polyethylene Glycol ◽  
B Cells ◽  
Islets Of Langerhans ◽  
Structural Features ◽  
Silicotungstic Acid ◽  
Frozen Sections ◽  
Glutaraldehyde Fixation ◽  
Ultrathin Frozen Sections ◽  
Direct Immersion ◽  
A And B Cells

A procedure is described for the preparation of ultrathin frozen sections of glutaraldehyde-fixed or unfixed islets of Langerhans by cryo-ultramicrotomy. Freezing of the tissue was accomplished by direct immersion of isolated islets in liquid nitrogen. Sectioning was performed at a specimen temperature of -80 °C and a knife temperature of -40 °C, the ribbon of sections being collected on a trough containing 60 % dimethyl sulphoxide. Staining was accomplished with 4 % silicotungstic acid and sections were protected from drying artifacts by rinsing with 0.5% polyethylene glycol. Even in tissue not subjected to prior glutaraldehyde fixation, most of the structural features of A and B cells were well preserved in frozen sections, which were obtained in a number and quality which should render them suitable for ultrastructural, cytochemical or radioautographic studies.

scholarly journals Characterization of voltage-dependent sodium and calcium channels in mouse pancreatic A- and B-cells

2006 ◽  
Vol 572 (3) ◽  
pp. 691-706 ◽  
Author(s):  
Sheila Vignali ◽  
Veronika Leiss ◽  
Rosi Karl ◽  
Franz Hofmann ◽  
Andrea Welling
Keyword(s):  
B Cells ◽  
Calcium Channels ◽  
Voltage Dependent ◽  
A And B Cells

INFLUENCE OF THE ISLET A AND B CELLS ON THE EXOCRINE PANCREATIC TISSUE IN THE DUCK

1962 ◽  
Vol 48 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
Arne Wallgren ◽  
Bo Hellman
Keyword(s):  
B Cells ◽  
Pancreatic Tissue ◽  
A And B Cells

Hyperplasia and hypertrophy of B cells in the islets of Langerhans in hamsters infected with the 139H strain of scrapie

1994 ◽  
Vol 110 (2) ◽  
pp. 169-183 ◽  
Author(s):  
X. Ye ◽  
R.I. Carp ◽  
Y. Yu ◽  
R. Kozielski ◽  
P. Kozlowski
Keyword(s):  
B Cells ◽  
Islets Of Langerhans

Neurohumoral regulation of the pancreatic islet A and B cells

Metabolism ◽  
1976 ◽  
Vol 25 (11) ◽  
pp. 1453-1456 ◽  
Author(s):  
Daniel Porte ◽  
Phillip H. Smith ◽  
John W. Ensinck
Keyword(s):  
B Cells ◽  
Pancreatic Islet ◽  
Neurohumoral Regulation ◽  
A And B Cells
Sign in / Sign up

Export Citation Format

Share Document