Induction of differentiation in the cultured F9 teratocarcinoma stem cells by triterpene acids

1994 ◽  
Vol 120 (9) ◽  
pp. 513-518 ◽  
Author(s):  
Ho-Young Lee ◽  
Hae-Young Chung ◽  
Ki-Heun Kim ◽  
Jung-Joon Lee ◽  
Kyu-Won Kim
Keyword(s):  
Stem Cells ◽  
Triterpene Acids ◽  
Induction Of Differentiation ◽  
F9 Teratocarcinoma

scholarly journals Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation.

1989 ◽  
Vol 108 (6) ◽  
pp. 2467-2476 ◽  
Author(s):  
S J Braunhut ◽  
L J Gudas ◽  
T Kurokawa ◽  
J Sasse ◽  
P A D'Amore
Keyword(s):  
Stem Cells ◽  
Cdna Probe ◽  
Biologically Active ◽  
Endothelial Cell Proliferation ◽  
Fibroblast Growth ◽  
Basic Fgf ◽  
F9 Cells ◽  
Parietal Endoderm ◽  
F9 Teratocarcinoma ◽  
Proliferation Assays

F9 teratocarcinoma stem cells treated with retinoic acid (RA) and dibutyryl cAMP (but2 cAMP) differentiate into embryonic parietal endoderm. Using heparin-affinity chromatography, endothelial cell proliferation assays, immunoprecipitation, and Western analysis with antibodies specific for acidic and basic fibroblast growth factors (FGFs), we detected biologically active FGF in F9 cells only after differentiation. A bovine basic FGF cDNA probe hybridized to 2.2-kb mRNAs in both F9 stem and parietal endoderm cells and to a 3.8-kb mRNA in F9 stem cells. A genomic DNA probe for acidic FGF hybridized to a 5.8-6.0-kb mRNA in both F9 stem and parietal endoderm cells, and to a 6.0-6.3-kb mRNA only in parietal endoderm cells. Although these FGF mRNAs were present in the stem cells, we could find no evidence that F9 stem cells synthesized FGFs, whereas differentiated F9 cells synthesized both acidic and basic FGF-like proteins. We conclude that biologically active factors with properties characteristic of acidic and basic FGF are expressed by F9 parietal endoderm cells after differentiation. Differentiating embryonic parietal endoderm thus may serve as a source of FGF molecules in the developing blastocyst, where these factors appear to play a central role in subsequent embryogenesis.

scholarly journals Differentiation Induction as a Response to Irradiation in Neural Stem Cells In Vitro

Cancers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 913 ◽  
Author(s):  
Jana Konířová ◽  
Lukáš Cupal ◽  
Šárka Jarošová ◽  
Anna Michaelidesová ◽  
Jana Vachelová ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Brain Cancer ◽  
Subventricular Zone ◽  
Transcriptional Activity ◽  
Radiation Induced ◽  
Induction Of Differentiation ◽  
Stem Cell Populations ◽  
Neurocognitive Decline

Radiotherapy plays a significant role in brain cancer treatment; however, the use of this therapy is often accompanied by neurocognitive decline that is, at least partially, a consequence of radiation-induced damage to neural stem cell populations. Our findings describe features that define the response of neural stem cells (NSCs) to ionizing radiation. We investigated the effects of irradiation on neural stem cells isolated from the ventricular-subventricular zone of mouse brain and cultivated in vitro. Our findings describe the increased transcriptional activity of p53 targets and proliferative arrest after irradiation. Moreover, we show that most cells do not undergo apoptosis after irradiation but rather cease proliferation and start a differentiation program. Induction of differentiation and the demonstrated potential of irradiated cells to differentiate into neurons may represent a mechanism whereby damaged NSCs eliminate potentially hazardous cells and circumvent the debilitating consequences of cumulative DNA damage.

scholarly journals RB2/p130 ectopic gene expression in neuroblastoma stem cells: evidence of cell-fate restriction and induction of differentiation

2001 ◽  
Vol 360 (3) ◽  
pp. 569 ◽  
Author(s):  
Francesco P. JORI ◽  
Umberto GALDERISI ◽  
Elena PIEGARI ◽  
Gianfranco PELUSO ◽  
Marilena CIPOLLARO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Fate ◽  
Ectopic Gene Expression ◽  
Induction Of Differentiation ◽  
Fate Restriction

112 Stepwise Induction Of Differentiation Of Human Induce Pluripotent Stem Cells Into Teno-lineage

2014 ◽  
Vol 48 (Suppl 2) ◽  
pp. A73-A74 ◽  
Author(s):  
Can Zhang ◽  
Huihua Yuan ◽  
Huanhuan Liu ◽  
Xiao Chen ◽  
Yanzhong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induction Of Differentiation

scholarly journals Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Sergio Mora-Castilla ◽  
Juan R. Tejedo ◽  
Rafael Tapia-Limonchi ◽  
Irene Díaz ◽  
Ana B. Hitos ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Histone H3 ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Directed Differentiation ◽  
Lineage Differentiation ◽  
Induction Of Differentiation ◽  
Differentiating Agent ◽  
Pluripotency Genes

The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition.

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