scholarly journals Cryogenic preservation of isolated rat Islets of Langerhans: Effect of cooling and warming rates

Diabetologia ◽  
1979 ◽  
Vol 16 (3) ◽  
pp. 195-199 ◽  
Author(s):  
H. L. Bank ◽  
R. F. Davis ◽  
D. Emerson
Keyword(s):  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Cryogenic preservation of isolated rat islets of Langerhans

Cryobiology ◽  
1976 ◽  
Vol 13 (6) ◽  
pp. 646-647
Author(s):  
Harvey Bank ◽  
Richard Davis ◽  
James Scoggin ◽  
Richard Weiss ◽  
Dorothy Noe
Keyword(s):  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Relationships between energy level and insulin secretion in isolated rat islets of langerhans

1992 ◽  
Vol 43 (8) ◽  
pp. 1859-1864 ◽  
Author(s):  
Mitsuaki Ohta ◽  
David Nelson ◽  
Jeanne M. Wilson ◽  
Martin D. Meglasson ◽  
Maria Erecińska
Keyword(s):  
Insulin Secretion ◽  
Energy Level ◽  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

scholarly journals Enhancement of glucagon secretion from isolated rat islets of Langerhans by phorbol 12-myristate 13-acetate

1986 ◽  
Vol 233 (1) ◽  
pp. 287-289 ◽  
Author(s):  
C S Hii ◽  
J Stutchfield ◽  
S L Howell
Keyword(s):  
Islets Of Langerhans ◽  
Glucagon Secretion ◽  
Glucose Stimulation ◽  
Rat Islets ◽  
Kinase C ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat ◽  
Stimulation Of

The phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA), at concentrations of 0.1 microM and above, stimulated secretion of glucagon and of insulin from isolated rat islets of Langerhans incubated in the presence of 5.5 mM-glucose. Stimulation of secretion of both hormones by 1 microM-PMA persisted in the absence of external Ca2+, and could be abolished by incubating the islets at 4 degrees C. These findings suggest a role of protein kinase C in the alpha-cell (and beta-cell) secretory mechanism.

scholarly journals Studies on the role of inositol trisphosphate in the regulation of insulin secretion from isolated rat islets of Langerhans

1985 ◽  
Vol 228 (3) ◽  
pp. 713-718 ◽  
Author(s):  
N G Morgan ◽  
G M Rumford ◽  
W Montague
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Islet Cells ◽  
Inositol Trisphosphate ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat ◽  
Stimulation Of

Glucose (20 mM) and carbachol (1 mM) produced a rapid increase in [3H]inositol trisphosphate (InsP3) formation in isolated rat islets of Langerhans prelabelled with myo-[3H]inositol. The magnitude of the increase in InsP3 formation was similar when either agent was used alone and was additive when they were used together. In islets prelabelled with 45Ca2+ and treated with carbachol (1 mM), the rise in InsP3 correlated with a rapid, transient, release of 45Ca2+ from the cells, consistent with mobilization of 45Ca2+ from an intracellular pool. Under these conditions, however, insulin secretion was not increased. In contrast, islets prelabelled with 45Ca2+ and exposed to 20mM-glucose exhibited a delayed and decreased 45Ca2+ efflux, but released 7-8-fold more insulin than did those exposed to carbachol. Depletion of extracellular Ca2+ failed to modify the increase in InsP3 elicited by either glucose or carbachol, whereas it selectively inhibited the efflux of 45Ca2+ induced by glucose in preloaded islets. Under these conditions, however, glucose was still able to induce a small stimulation of the first phase of insulin secretion. These results demonstrate that polyphosphoinositide metabolism, Ca2+ mobilization and insulin release can all be dissociated in islet cells, and suggest that glucose and carbachol regulate these parameters by different mechanisms.

Intracellular Ca2+ mobilization in isolated rat islets of Langerhans

1987 ◽  
Vol 15 (5) ◽  
pp. 939-940
Author(s):  
NOEL G. MORGAN ◽  
ROGER D. HURST ◽  
GWYNETH M. RUMFORD ◽  
WILLIAM MONTAGUE
Keyword(s):  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Alloxan Stimulation and Inhibition of Insulin Release from Isolated Rat Islets of Langerhans

Diabetes ◽  
1978 ◽  
Vol 27 (12) ◽  
pp. 1205-1214 ◽  
Author(s):  
D. C. Weaver ◽  
M. L. McDaniel ◽  
S. P. Naber ◽  
C. D. Barry ◽  
P. E. Lacy
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Effect of L-Asparaginase on Insulin Secretion from Isolated Rat Islets of Langerhans

1991 ◽  
Vol 35 (3-4) ◽  
pp. 155-160 ◽  
Author(s):  
J.M. Pou ◽  
T. Cervera ◽  
A. Perez ◽  
M.A. Ortiz ◽  
J.A. Arroyo
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat
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