Preparation of the tritiated molecular forms of gangliosides with homogeneous long chain base composition

1984 ◽  
Vol 1 (2) ◽  
pp. 111-121 ◽  
Author(s):  
Guiliano Gazzotti ◽  
Sandro Sonnino ◽  
Riccardo Ghidoni ◽  
Paolo Orlando ◽  
Guido Tettamanti
Keyword(s):  
Base Composition ◽  
Molecular Forms ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Long chain base composition of strains of three species ofSphingobacteriumgen. nov.

1982 ◽  
Vol 15 (4) ◽  
pp. 303-307 ◽  
Author(s):  
Ikuya Yano ◽  
Ikuko Tomiyasu ◽  
Eiko Yabuuchi
Keyword(s):  
Base Composition ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria

FEBS Letters ◽  
2001 ◽  
Vol 488 (3) ◽  
pp. 160-164 ◽  
Author(s):  
D Ardail ◽  
I Popa ◽  
K Alcantara ◽  
A Pons ◽  
J.P Zanetta ◽  
...  
Keyword(s):  
Rat Liver ◽  
Base Composition ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Chain Base ◽  
Long Chain Base ◽  
Long Chain

scholarly journals Matrix-assisted laser desorption ionization time-of-flight mass spectrometric analysis of glycosphingolipids including gangliosides.

1998 ◽  
Vol 45 (4) ◽  
pp. 987-999 ◽  
Author(s):  
T Taketomi ◽  
A Hara ◽  
K Uemura ◽  
E Sugiyama
Keyword(s):  
Base Composition ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Chain Base ◽  
Long Chain Base ◽  
The Brain ◽  
Long Chain ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Long chain base compositions of gangliosides containing mainly stearic acid could be determined without any chemical modification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS). The analytical results for the long chain base compositions of various samples of GM1 from the brain tissues of patients with different diseases at different ages confirmed that the proportion of d20:1 (icosasphingosine) and d20 (icosa-sphinganine) of the total sphingosine bases increased quickly until adolescent or adult age and then remained constant slightly exceeding 50%; this value was evidently higher than the proportion of d20:1 and d20 of GM1 in various adult mammalian brains. A long chain base composition of GM1 from the brain tissue of a patient with infantile type of GM1-gangliosidosis at 4y2m was abnormal and so was in two sibling patients with Spielmeyer-Vogt type of juvenile amaurotic idiocy at 19y and 21y in spite of that in the latter there was no accumulation of GM1 in the brain tissue. On the other hand, a patient with adult type of GM1 gangliosidosis at 66y showed a local accumulation of GM1 in the putamen and caudate nucleus, but its long chain base composition was found to be normal. It was of interest that the white matter of Eker rat with hereditary renal carcinoma contained a large amount of plasmalocerebroside as compared with the amount of cerebroside and sphingomyelin. The individual molecular species of plasmalocerebroside were identified by DE MALDI-TOF MS.

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