S20.14 A monoclonal antibody, RGM41, specific for the mucin derived from the gland mucous cells of mammalian stomach

1993 ◽  
Vol 10 (4) ◽  
pp. 346-346
Author(s):  
K. Ishihara ◽  
M. Kurihara ◽  
Y. Goso ◽  
H. Ota ◽  
T. Katsuyama ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mucous Cells ◽  
Mammalian Stomach

scholarly journals Peripheral α-linked N-acetylglucosamine on the carbohydrate moiety of mucin derived from mammalian gastric gland mucous cells: epitope recognized by a newly characterized monoclonal antibody

1996 ◽  
Vol 318 (2) ◽  
pp. 409-416 ◽  
Author(s):  
Kazuhiko ISHIHARA ◽  
Makoto KURIHARA ◽  
Yukinobu GOSO ◽  
Tsukiko URATA ◽  
Hiroyoshi OTA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gastric Gland ◽  
Peripheral Region ◽  
Carbohydrate Moiety ◽  
Dependent Manner ◽  
Mucous Cells ◽  
Concentration Dependent Manner ◽  
Mucosal Layer ◽  
Rat Gastric Mucosa ◽  
Derivatives Of

To obtain a tool to study the structural characterization and the detection of mucin derived from the gastric gland mucous cells, we developed a monoclonal antibody, designated HIK1083, against mucin purified from rat gastric mucosa. In an ELISA, HIK1083 reacted strongly with the mucin purified from a deep layer of the corpus and antrum but only slightly reacted with that obtained from the surface mucosal layer. The reaction of mucin and HIK1083 was inhibited by the oligosaccharides obtained by the alkaline borohydride reduction of antigenic mucin. Two purified oligosaccharide alditols reacting with the monoclonal antibody obtained from the antigenic mucin had one and two peripheral α-linked N-acetylglucosamine residues, respectively, according to the evidence from NMR spectroscopy. Moreover, among the commercially available p-nitrophenyl derivatives of monosaccharides, only p-nitrophenyl-N-acetyl-α-d-glucosaminide inhibited the reaction of this monoclonal antibody and the antigenic mucin in a concentration-dependent manner. These results, as well as the immunohistochemical observations, indicate that α-linked N-acetylglucosamine residues are specifically attached to the peripheral region of the carbohydrate moiety of the mucin synthesized in and secreted from the gastric-gland-type cells, and indicate that the monoclonal antibody HIK1083 recognizes this structure.

Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: Its pathophysiological changes in human gastric juice

2007 ◽  
Vol 377 (1-2) ◽  
pp. 261-267 ◽  
Author(s):  
Seiko Kubota ◽  
Kazuyoshi Yamauchi ◽  
Toshiko Kumagai ◽  
Mitsutoshi Sugano ◽  
Kenji Kawasaki ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Gastric Juice ◽  
Mucous Cells ◽  
Human Gastric Juice

Cytoplasmic Invaginations in Trophoblasts and Epithelial Cells of Rat

1971 ◽  
Vol 29 ◽  
pp. 524-525
Author(s):  
Iracema M. Baccarini
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Nuclear Inclusions ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Cervical Epithelium ◽  
Intact Membrane ◽  
Microscopic Studies ◽  
Abnormal Cells ◽  
Nuclear Features

Some morphological nuclear features (invaginations) in normal and abnormal cells have been described in several electron microscopic studies. They have been referred to by others as blebs, loops, pockets, sheets, bodies, nuclear inclusions and cytoplasmic invaginations. Identical appearing structures were found in cells of the uterine cervical epithelium, in trophoblasts of blastocysts and in trophoblasts of rat placenta.Methods. Uterine cervix (normal rats), rat placenta (9-10 days gestation) and blastocyst were placed in 3% glutarahdehyde for 3 hours. The tissue was washed in phosphate buffer for 24 hours, postfixed in 1%. buffered osmium tetroxide for 1-2 hours and embedded in epon araldite. Sections were double stained with uranyl acetate and lead citrate and viewed in E. M. Siemens 200.Observations. Nuclear invaginations were found in basal, parabasal and mucous cells of the cervix epithelium, in trophoblasts of blastocyst and in trophoblasts of placenta. An oval, round or elongated invagination contained heterogenously cytoplasm surrounded by a double intact membrane; usually several invaginations were found in the same nucleus.

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

Identification of the pulmonary syndrome hantavirus by direct and colloidal gold immune Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 274-275
Author(s):  
C. D. Humphrey ◽  
C.S. Goldsmith ◽  
L. Elliott ◽  
S.R. Zaki
Keyword(s):  
Electron Microscopy ◽  
United States ◽  
Monoclonal Antibody ◽  
Colloidal Gold ◽  
Phosphotungstic Acid ◽  
Uranyl Acetate ◽  
Hantavirus Pulmonary Syndrome ◽  
Deer Mice ◽  
Immune Electron Microscopy ◽  
Negative Stain

An outbreak of unexplained acute pulmonary syndrome with high fatality was recognized in the spring of 1993 in the southwestern United States. The cause of the illness was quickly identified serologically and genetically as a hantavirus and the disease was named hantavirus pulmonary syndrome (HPS). Recently, the virus was isolated from deer mice which had been trapped near the homes of HPS patients, and cultivated in Vero E6 cells. We identified the cultivated virus by negative-stain direct and colloidal gold immune electron microscopy (EM).Virus was extracted, clarified, and concentrated from unfixed and 0.25% glutaraldehyde fixed supernatant fluids of infected Vero E6 cells by a procedure described previously. Concentrated virus suspensions tested by direct EM were applied to glow-discharge treated formvar-carbon filmed grids, blotted, and stained with 0.5% uranyl acetate (UA) or with 2% phosphotungstic acid (PTA) pH 6.5. Virus suspensions for immune colloidal gold identification were adsorbed similarly to filmed grids but incubated for 1 hr on drops of 1:50 diluted monoclonal antibody to Prospect Hill virus nucleoprotein or with 1:50 diluted sera from HPS virus infected deer mice.

IgE and monoclonal antibody binding by the mite allergen Der p 7

1996 ◽  
Vol 26 (3) ◽  
pp. 308-315 ◽  
Author(s):  
H.-D. SHEN ◽  
K. Y. CHUA ◽  
W. L. LIN ◽  
H. L. CHEN ◽  
K.-H. HSIEH ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mite Allergen ◽  
Antibody Binding ◽  
Monoclonal Antibody Binding

Quantification in mass units of Bet v 1, the main allergen of Betula verrucosa pollen, by a monoclonal antibody based-ELISA

1997 ◽  
Vol 27 (8) ◽  
pp. 926-931 ◽  
Author(s):  
J. RAMiREZ ◽  
J. A. CARPIZO ◽  
H. IPSEN ◽  
J. CARREIRA ◽  
M. LOMBARDERO
Keyword(s):  
Monoclonal Antibody ◽  
Bet V 1 ◽  
Betula Verrucosa

Helicobacter pylori infection produces expression of secretory component in gastric mucous cells

2001 ◽  
Vol 120 (5) ◽  
pp. A708-A709
Author(s):  
T KANEKO ◽  
H OTA ◽  
M HAYAMA ◽  
K NAKAJIMA ◽  
A YOSHIZAWA ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretory Component ◽  
Helicobacter Pylori Infection ◽  
Mucous Cells ◽  
Gastric Mucous
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