S5.6 Sponge cell aggregation — The functional implication of carbohydrate epitopes in cellular interactions

1993 ◽  
Vol 10 (4) ◽  
pp. 251-252
Author(s):  
D. Spillmann ◽  
K. H⫗aard ◽  
J. Thomas-Oates ◽  
J. F. G. Vliegenthart ◽  
M. M. Burger ◽  
...  
Keyword(s):  
Cell Aggregation ◽  
Sponge Cell ◽  
Cellular Interactions ◽  
Functional Implication ◽  
Carbohydrate Epitopes

The release of soluble H-2 alloantigens during disaggregation of mouse embryo tissue by a chelating agent

Development ◽  
1966 ◽  
Vol 16 (3) ◽  
pp. 519-530
Author(s):  
Michael Edidin
Keyword(s):  
Molecular Weight ◽  
Ethylenediaminetetraacetic Acid ◽  
Divalent Cations ◽  
Weight Bearing ◽  
Chelating Agent ◽  
Cell Aggregation ◽  
Sponge Cell ◽  
Antigenic Sites ◽  
Dissociated Cells ◽  
Embryo Tissue

Treatment with chelating agents binding divalent cations has been found to effect the dissociation of a variety of tissues of both embryo and adult animals (reviewed in Steinberg, 1958). In the course of dissociation it appears that materials are released from cell surfaces which play a part in their specific adhesion, and which may be shown experimentally to promote selectively the re-aggregation of dissociated cells (Humphreys, 1963; Moscona, 1963). The extracted materials appear to be glycoprotein complexes (Humphreys, 1965), made up of fairly small subunits, estimated to be of 13000–20000 molecular weight (Margoliash et al. 1965). Units of about the same size appear to be the antigenic sites involved in the blocking of sponge cell aggregation by rabbit anti-sponge serum, specific for a given sponge species (MacLennan, 1963). I shall here present evidence that materials of similar molecular weight bearing immunological specificities of the H-2 alloantigen system are released from the tissues of certain mouse embryos during the course of their dissociation by the chelating agent Versene (ethylenediaminetetraacetic acid).

Role of Different Sponge Cell Types in Species Specific Cell Aggregation

1971 ◽  
Vol 230 (12) ◽  
pp. 126-128 ◽  
Author(s):  
H. A. JOHN ◽  
M. S. CAMPO ◽  
A. M. MACKENZIE ◽  
R. B. KEMP
Keyword(s):  
Cell Aggregation ◽  
Cell Types ◽  
Specific Cell ◽  
Sponge Cell ◽  
Species Specific

Sponge cell aggregation: checkpoints in development indicate a high level of organismal complexity

2014 ◽  
Vol 134 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Dafne Eerkes-Medrano ◽  
Colette J. Feehan ◽  
Sally P. Leys
Keyword(s):  
Cell Aggregation ◽  
Sponge Cell ◽  
Organismal Complexity ◽  
High Level

Hepatocyte differentiation initiates during endodermal-mesenchymal interactions prior to liver formation

Development ◽  
1991 ◽  
Vol 113 (1) ◽  
pp. 217-225 ◽  
Author(s):  
S. Cascio ◽  
K.S. Zaret
Keyword(s):  
General Pattern ◽  
Cell Aggregation ◽  
Cell Types ◽  
Specific Gene ◽  
Mrna Levels ◽  
Cellular Interactions ◽  
Hepatocyte Differentiation ◽  
Endodermal Cells ◽  
Tissue Matrix ◽  
Albumin Mrna

Previous studies with embryonic tissue explants showed that cellular interactions with mesenchyme are required for endodermal cells to differentiate into hepatocytes. However, these studies assayed hepatocyte characteristics that were evident after days of culture, leaving open the question of whether the primary inductive interactions initiated hepatocyte differentiation, or whether subsequent steps, such as may occur during cell aggregation to form the liver, were necessary. Using the technique of in situ hybridization, we find that serum albumin mRNA, a liver-specific gene product, is first detected in hepatic precursor cells of the endoderm as early as 9.5 days of mouse embryo development, a full day prior to cell aggregation and liver formation. The endodermal cells express albumin mRNA upon migration into strands of connective tissue matrix within mesenchyme. Thus, the onset of differentiation of the endoderm is coincident with its interaction with mesenchyme. Early albumin transcripts are initiated at the same site of the albumin promoter as in adult hepatocytes, suggesting that at least a subset of the transcription factors that control albumin transcription in the adult may be involved in executing the early steps of hepatic determination. We also observe a sharp increase in albumin mRNA levels shortly after the definitive formation of the liver, apparently reflecting cell interactions that enhance hepatocyte differentiation. Hepatocyte differentiation is therefore similar in several respects to pancreatic exocrine cell development, and may represent a general pattern for gut-derived tissues. For both cell types, early interactions with mesenchyme are coincident with the initial expression of differentiated gene products at a low level in proliferating endoderm, and the initial pattern of expression is amplified upon organ formation.

A role for sulfated polysaccharide recognition in sponge cell aggregation

1987 ◽  
Vol 170 (2) ◽  
pp. 381-401 ◽  
Author(s):  
Deirdre R. Coombe ◽  
Karen B. Jakobsen ◽  
Christopher R. Parish
Keyword(s):  
Cell Aggregation ◽  
Sulfated Polysaccharide ◽  
Sponge Cell
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