Non-myelin-forming Schwann cells proliferate rapidly during Wallerian degeneration in the rat sciatic nerve

1989 ◽  
Vol 18 (2) ◽  
pp. 185-192 ◽  
Author(s):  
A. Clemence ◽  
R. Mirsky ◽  
K. R. Jessen
Keyword(s):  
Sciatic Nerve ◽  
Schwann Cells ◽  
Wallerian Degeneration ◽  
Rat Sciatic Nerve

scholarly journals Extracellular matrix in regenerating rat sciatic nerve: a comparative study on the localization of laminin, hyaluronic acid, and chondroitin sulfate proteoglycans, including versican.

1993 ◽  
Vol 41 (4) ◽  
pp. 593-599 ◽  
Author(s):  
A Tona ◽  
G Perides ◽  
F Rahemtulla ◽  
D Dahl
Keyword(s):  
Extracellular Matrix ◽  
Sciatic Nerve ◽  
Chondroitin Sulfate ◽  
Schwann Cells ◽  
Wallerian Degeneration ◽  
Polyclonal Antibodies ◽  
Chondroitin Sulfate Proteoglycans ◽  
Distal Stump ◽  
Double Labeling ◽  
Rat Sciatic Nerve

Using a glial hyaluronate-binding protein as a probe, monoclonal antibodies against versican and ABC digested chondroitin sulfate proteoglycans, and polyclonal antibodies against laminin, we localized these extracellular matrix (ECM) components in the endoneurium of the adult rat sciatic nerve. During Wallerian degeneration caused by nerve crushing, the staining pattern of these ECM elements changed dramatically. In the first stages and up to 5 days after injury, the tubular endoneurial structures remained the same as in control nerves. Ten days after crush, the bands of Büngner formed by proliferating Schwann cells in the distal stump of crushed nerves stained diffusely for hyaluronate, laminin, and chondroitin sulfate. Regenerating axons were demonstrated in this location by double-labeling experiments with neurofilament antibodies. Conversely, staining with antibodies against versican, a hyaluronate binding proteoglycan, was reduced and the bands of Büngner were not stained. After 30 days the endoneurial tubes made their reappearance and, as in normal nerve, they stained for hyaluronate, laminin, versican, and chondroitin sulfate. It is concluded that proliferating Schwann cells in peripheral nerve undergoing Wallerian degeneration are capable of producing several endoneurial ECM constituents, versican being a notable exception.

A Neuropathy in Goats Caused by Experimental Coyotillo (Karwinskia humboldtiana) Poisoning

1970 ◽  
Vol 7 (5) ◽  
pp. 420-434 ◽  
Author(s):  
K. M. Charlton ◽  
K. R. Pierce
Keyword(s):  
Electron Microscopy ◽  
Sciatic Nerve ◽  
Schwann Cells ◽  
Active Transport ◽  
Peripheral Nerves ◽  
Wallerian Degeneration ◽  
Light And Electron Microscopy ◽  
Segmental Demyelination ◽  
Femoral Site ◽  
Oral Doses

Lesions in peripheral nerves from 12 goats poisoned experimentally with coyotillo were studied by light and electron microscopy. The goats were poisoned with daily oral doses of the ground coyotillo fruits and killed at various times after the first day of dosing. Lesions at a mid-femoral site of the sciatic nerve included swelling of Schwann cells, degeneration of mitochondria, depletion of glycogen, splitting of myelin, segmental demyelination, and Wallerian degeneration. The results were suggestive of primary mitochondrial injury in Schwann cells with resultant impaired active transport, intracellular edema, splitting of myelin, and segmental demyelination.

Perineurial permeability to sodium during Wallerian degeneration in rat sciatic nerve

1992 ◽  
Vol 581 (2) ◽  
pp. 327-333 ◽  
Author(s):  
Ananda Weerasuriya ◽  
Charles H. Hockman
Keyword(s):  
Sciatic Nerve ◽  
Wallerian Degeneration ◽  
Rat Sciatic Nerve

Signal flow and pathways in response to early Wallerian degeneration after rat sciatic nerve injury

2013 ◽  
Vol 536 ◽  
pp. 56-63 ◽  
Author(s):  
Meiyuan Li ◽  
Weimin Guo ◽  
Pingan Zhang ◽  
Huaiqin Li ◽  
Xiaosong Gu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Injury ◽  
Wallerian Degeneration ◽  
Sciatic Nerve Injury ◽  
Signal Flow ◽  
Rat Sciatic Nerve

scholarly journals Identification of novel cell-adhesion molecules in peripheral nerves using a signal-sequence trap

2005 ◽  
Vol 2 (1) ◽  
pp. 27-38 ◽  
Author(s):  
IVO SPIEGEL ◽  
KONSTANTIN ADAMSKY ◽  
MENAHEM EISENBACH ◽  
YAEL ESHED ◽  
ADRIAN SPIEGEL ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Sciatic Nerve ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Schwann Cells ◽  
Cell Adhesion Molecules ◽  
Signal Sequence ◽  
Cdna Libraries ◽  
Rat Sciatic Nerve ◽  
Myelinated Nerves

The development and maintenance of myelinated nerves in the PNS requires constant and reciprocal communication between Schwann cells and their associated axons. However, little is known about the nature of the cell-surface molecules that mediate axon–glial interactions at the onset of myelination and during maintenance of the myelin sheath in the adult. Based on the rationale that such molecules contain a signal sequence in order to be presented on the cell surface, we have employed a eukaryotic-based, signal-sequence-trap approach to identify novel secreted and membrane-bound molecules that are expressed in myelinating and non-myelinating Schwann cells. Using cDNA libraries derived from dbcAMP-stimulated primary Schwann cells and 3-day-old rat sciatic nerve mRNAs, we generated an extensive list of novel molecules expressed in myelinating nerves in the PNS. Many of the identified proteins are cell-adhesion molecules (CAMs) and extracellular matrix (ECM) components, most of which have not been described previously in Schwann cells. In addition, we have identified several signaling receptors, growth and differentiation factors, ecto-enzymes and proteins that are associated with the endoplasmic reticulum and the Golgi network. We further examined the expression of several of the novel molecules in Schwann cells in culture and in rat sciatic nerve by primer-specific, real-time PCR and in situ hybridization. Our results indicate that myelinating Schwann cells express a battery of novel CAMs that might mediate their interactions with the underlying axons.

scholarly journals Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve.

1992 ◽  
Vol 89 (18) ◽  
pp. 8827-8831 ◽  
Author(s):  
M. L. Feltri ◽  
S. S. Scherer ◽  
L. Wrabetz ◽  
J. Kamholz ◽  
M. E. Shy
Keyword(s):  
Sciatic Nerve ◽  
Schwann Cells ◽  
Rat Sciatic Nerve
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