Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase

1996 ◽  
Vol 16 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Eduardo M. R. Reis ◽  
Carolyn W. Slayman ◽  
Sergio Verjovski-Almeida
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Recombinant Protein ◽  
Ligand Binding ◽  
Heterologous Expression ◽  
Eukaryotic Expression ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Expression Systems ◽  
Eukaryotic Expression Systems

In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca2+-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.

scholarly journals A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
B. Owczarek ◽  
A. Gerszberg ◽  
K. Hnatuszko-Konka
Keyword(s):  
Recombinant Protein ◽  
Recombinant Proteins ◽  
Eukaryotic Expression ◽  
Expression Systems ◽  
Expression And Purification ◽  
Advantages And Disadvantages ◽  
Protein Molecules ◽  
The Face ◽  
Mature Field ◽  
Eukaryotic Expression Systems

Recombinant proteins are produced for various applications in laboratory and industrial settings. Among them, therapeutic applications have evolved into a mature field in recent years, affecting the face of contemporary medical treatment. This, in turn, has stimulated an ever-greater need for innovative technologies for the description, expression, and purification of recombinant protein biopharmaceuticals. Therefore, many biopharmaceuticals are synthesized in heterologous systems to obtain satisfactory yields that cannot be provided by natural sources. As more than 35 years has passed since the first recombinant biopharmaceutical (human insulin) successfully completed clinical trials in humans, we provide a brief review of the available prokaryotic and eukaryotic expression systems, listing the advantages and disadvantages of their use. Some examples of therapeutic proteins expressed in heterologous hosts are also provided. Moreover, technologies for the universal extraction of protein molecules are mentioned here, as is the methodology of their purification.

scholarly journals Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems

2019 ◽  
Vol 294 (17) ◽  
pp. 6986-7001 ◽  
Author(s):  
Rachel J. Harding ◽  
Peter Loppnau ◽  
Suzanne Ackloo ◽  
Alexander Lemak ◽  
Ashley Hutchinson ◽  
...  
Keyword(s):  
Eukaryotic Expression ◽  
Expression Systems ◽  
Eukaryotic Expression Systems
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