An ultrastructural study of nerve and glial cells by freeze-substitution

1980 ◽  
Vol 9 (2) ◽  
pp. 243-254 ◽  
Author(s):  
Nobutaka Hirokawa ◽  
Takaaki Kirino
Keyword(s):  
Glial Cells ◽  
Ultrastructural Study ◽  
Freeze Substitution

Ultrastructural study of the fission yeast Schizosaccharomyces pombe nucleolus by freeze substitution

1995 ◽  
Vol 84 (3) ◽  
pp. 228-228
Author(s):  
Leger Isabelle ◽  
Jacqueline Noaillac-Depeyre ◽  
Marie-Pierre Gulli ◽  
Michèle Caizergues-Ferrer ◽  
Nicole Gas
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Ultrastructural Study ◽  
Freeze Substitution

An Ultrastructural Study of the Lateral Vestibular Nucleus in the Rat: Changes in Glial Cells Following Partial Deafferentation

1973 ◽  
Vol 31 ◽  
pp. 658-659
Author(s):  
J. E. Johnson
Keyword(s):  
Corpus Callosum ◽  
Glial Cells ◽  
Ultrastructural Study ◽  
Vestibular Nucleus ◽  
Vestibular Nuclei ◽  
Lateral Vestibular Nucleus ◽  
Light Medium

Mori and Leblond have reported that oligodendrocytes in the corpus callosum of rats may be classified into three types consisting of light, medium and dark. The light oligodendrocytes are the largest, and the dark ones are the smallest. In the present study, oligodendrocytes of different sizes and densities were found to occur in the normal lateral vestibular nucleus of the rat. Here, as reported in the corpus callosum, the darkest cells are the smallest. The largest of the oligodendrocytes in the lateral vestibular nucleus have a density which approaches that of astrocytes, which are also present throughout the nucleus.The lateral vestibular nuclei of 18 rats were partially deaffer-entated by aspirating the anterior vermis of the cerebellum, from which the nucleus receives input.

An ultrastructural study of ascospore delimitation and maturation in Ascodesmis nigricana using freeze substitution fixation

1990 ◽  
Vol 48 (3) ◽  
pp. 630-631
Author(s):  
C. W. Mints ◽  
E. A. Richardson ◽  
J. Kimbrough
Keyword(s):  
Ultrastructural Study ◽  
Freeze Substitution ◽  
Chemical Fixation ◽  
Dialysis Membrane ◽  
Liquid Propane

Fungi belonging to the class Ascomycetes are characterized by the production of sexually derived spores termed ascospores inside a microscopic, sac-like structure termed an ascus. In the Euascomycetidae, typically uninucleate spore initials are delimited within the ascus as the result of the invagination of membranes that arise from the so-called "ascus vesicle", a discontinuous cylinder of two closely spaced unit membranes that develops around the extreme periphery of the ascus. To date, there are conflicting data regarding the origin of the ascus vesicle. We therefore decided to address the problem using freeze substitution fixation. The advantages of this procedure over conventional chemical fixation protocols for preservation of ultrastructural details in fungi have been well-documented. In this study small pieces of dialysis membrane bearing developing ascocarps of Ascodesmis nigricans were plunged into liquid propane and processed for TEM according to the procedures of Hoch and Mints et al.

Glial cells in the lizardGallotia galloti subpallial nuclei during ontogeny: An ultrastructural study

1997 ◽  
Vol 233 (1) ◽  
pp. 1-13 ◽  
Author(s):  
C. Yanes ◽  
M. Monzón-Mayor ◽  
G. Gombos ◽  
J. Renau Piqueras ◽  
R.R. Sturrock
Keyword(s):  
Glial Cells ◽  
Ultrastructural Study
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