Effects of platelet activating factor on mouse sperm function

1992 ◽  
Vol 9 (5) ◽  
pp. 447-453 ◽  
Author(s):  
Kazuo Sengoku ◽  
Mutsuo Ishikawa ◽  
Kenichi Tamate ◽  
Tetsuya Shimizu
Keyword(s):  
Platelet Activating Factor ◽  
Sperm Function ◽  
Mouse Sperm

Sperm DNA fragmentation: awakening the sleeping genome

2007 ◽  
Vol 35 (3) ◽  
pp. 626-628 ◽  
Author(s):  
J.A. Shaman ◽  
Y. Yamauchi ◽  
W.S. Ward
Keyword(s):  
Dna Synthesis ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Sperm Function ◽  
Sperm Dna Fragmentation ◽  
Dna Breaks ◽  
Mouse Sperm ◽  
Sperm Dna ◽  
Total Digestion ◽  
Mammalian Spermatozoa

We have recently demonstrated that mammalian spermatozoa have the ability to degrade their DNA by a mechanism that is similar to apoptosis in somatic cells. When this mechanism is activated, the DNA is first degraded into loop-sized fragments by TOP2B (topoisomerase IIB). This degradation, termed sperm chromatin fragmentation, can be reversed by EDTA, which causes TOP2B to religate the double-stranded breaks it originally produced. Under certain conditions, a nuclease then degrades the sperm DNA further, digesting the entire sperm genome. When mouse spermatozoa which have been treated to induce TOP2B-mediated DNA breaks are injected into oocytes, the paternal DNA is specifically and completely degraded. This total digestion of paternal DNA occurs at the time of DNA synthesis initiation. In the present study, we explore the significance of an active TOP2B in the nucleus for mouse sperm function.

scholarly journals Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2

2015 ◽  
Vol 35 (1) ◽  
pp. 374-385 ◽  
Author(s):  
Tao Luo ◽  
Qian-xing Zou ◽  
Yuan-qiao He ◽  
Hua-feng Wang ◽  
Na Li ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Male Reproduction ◽  
In Vitro Toxicity ◽  
Sperm Function ◽  
Ionophore A23187 ◽  
Epididymal Sperm ◽  
Mouse Sperm ◽  
Extracellular Signal ◽  
Mature Mouse

Background: Matrine is a bioactive alkaloid that has a variety of pharmacological effects and is widely used in Chinese medicine. However, its effects on male reproduction are not well known. In this study, we aimed to investigate the in vitro toxicity of matrine on mature mouse sperm. Methods: Mouse cauda epididymal sperm were exposed to matrine (10-200 µM) in vitro. The viability, motility, capacitation, acrosome reaction and fertilization ability of the mouse sperm were examined. Furthermore, the intracellular calcium concentration ([Ca2+]i), calcium (Catsper) and potassium (Ksper) currents, and phosphorylation of extracellular signal regulated kinases 1/2 (p-ERK1/2) of the sperm were analyzed. Results: After exposure to 100 µM or more of matrine, mouse cauda epididymal sperm exhibited a significant reduction in total motility, progressive motility, linear velocity and acrosome reaction rate induced by Ca2+ ionophore A23187. As a result, the fertilization ability of mouse sperm was remarkably decreased by matrine. Our data further demonstrated that matrine significantly reduced sperm [Ca2+]i and [Ca2+]i-related p-ERK1/2; however, both the CatSper and KSper currents, which are thought to interactively regulate Ca2+ influx in sperm, were not affected by matrine. Conclusion: Our findings indicate that matrine inhibits mouse sperm function by reducing sperm [Ca2+]i and suppressing the phosphorylation of ERK1/2.

scholarly journals Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation

2007 ◽  
Vol 100 (4) ◽  
pp. 941-951 ◽  
Author(s):  
Yen Hua Huang ◽  
Yee Hsiung Chen ◽  
Chun Mao Lin ◽  
Yi Yun Ciou ◽  
Shin Peih Kuo ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Platelet Activating Factor ◽  
Sperm Capacitation ◽  
Mouse Sperm ◽  
Suppression Effect

Andrology: Effects of platelet activating factor on human sperm function in vitro

1993 ◽  
Vol 8 (9) ◽  
pp. 1443-1447 ◽  
Author(s):  
K. Sengoku ◽  
K. Tamate ◽  
Y. Takaoka ◽  
M. Ishikawa
Keyword(s):  
Platelet Activating Factor ◽  
Human Sperm ◽  
Sperm Function

scholarly journals Expression and Possible Role of Nicotinic Acetylcholine Receptor ε Subunit (AChRe) in Mouse Sperm

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 46
Author(s):  
Yusei Makino ◽  
Yuuki Hiradate ◽  
Kohei Umezu ◽  
Kenshiro Hara ◽  
Kentaro Tanemura
Keyword(s):  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Acrosome Reaction ◽  
Sperm Function ◽  
Nicotinic Acetylcholine ◽  
Mouse Sperm ◽  
Mammalian Sperm ◽  
Specific Antagonist ◽  
Sperm Acrosome Reaction

The nicotinic acetylcholine receptor (nAChR) is one of the receptors of acetylcholine (ACh), and nicotine (NIC) acts as an agonist of this receptor. Among the nAChR subunits, we found that the ε subunit (AChRe) had approximately 10 to 1000 times higher level of mRNA expression in mouse testes than the other subunits. In this study, we aimed to elucidate the expression and localization of AChRe in the testes and spermatozoa of mice and clarify the effect of AChRe on sperm function. Immunocytochemistry showed that AChRe was expressed in the murine testes and spermatozoa. We found that AChRe was localized only in elongated spermatids from step 12 onwards in the testes. In spermatozoa, AChRe was localized in the head, especially in the anterior region of the acrosome, but only approximately 50% of spermatozoa showed this immunoreactivity. Additionally, we analyzed the effects of ACh and NIC on sperm acrosome reaction (AR) and found that both ACh and NIC suppressed the AR rate, which was restored by an AChRe-specific antagonist. These results suggest that AChRe may be a regulator of mammalian sperm AR.

scholarly journals Involvement of fibroblast growth factor 2 (FGF2) and its receptors in the regulation of mouse sperm physiology

Reproduction ◽  
2018 ◽  
Vol 156 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Lucía Saucedo ◽  
Cristian Sobarzo ◽  
Nicolás G Brukman ◽  
Héctor A Guidobaldi ◽  
Livia Lustig ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Sperm Motility ◽  
Cellular Proliferation ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Fibroblast Growth Factor 2 ◽  
Sperm Function ◽  
Fibroblast Growth ◽  
Mouse Sperm

Fibroblast growth factor 2 (FGF2) and its receptors (FGFRs) have been described in several tissues, where they regulate cellular proliferation, differentiation, motility and apoptosis. Although FGF2/FGFRs expression in the male reproductive tract has been reported, there is scarce evidence on their presence in the female reproductive tract and their involvement in the modulation of sperm function. Therefore, the objective of this study was to determine the expression of FGF2 in the female reproductive tract and to assess the role of the FGF2/FGFRs system in the regulation of sperm physiology using the murine model. FGF2 was detected in uterus and oviduct protein extracts, and it was immunolocalized in epithelial cells of the uterus,isthmusandampulla, as well as in thecumulus oophorus-oocyte complex. The receptors FGFR1, FGFR2, FGFR3 and FGFR4 were immunodetected in the flagellum and acrosomal region of sperm recovered from thecaudaepididymis. Analysis of testis sections showed the expression of FGFRs in germ cells at different stages of the spermatogenesis, suggesting the testicular origin of the sperm FGFRs. Sperm incubation with recombinant FGF2 (rFGF2) led to increased sperm motility and velocity and to enhanced intracellular Ca2+levels and acrosomal loss compared to the control. In conclusion, this study shows that FGF2 is expressed in tissues of the female reproductive tract. Also, the fact that functional FGFRs are present in mouse sperm and that rFGF2 affects sperm motility and acrosomal exocytosis, suggests the involvement of this system in thein vivoregulation of sperm function.

scholarly journals Effects of aqueous crude extract ofEcheveria gibbifloraon mouse sperm function

2016 ◽  
Vol 62 (5) ◽  
pp. 343-352 ◽  
Author(s):  
Joaquín Cordero-Martínez ◽  
Charmina Aguirre-Alvarado ◽  
Jessica Gabriela Guzmán-Soriano ◽  
Cinthia Erika Sánchez-Arroyo ◽  
Juan Carlos Flores-Alonso ◽  
...  
Keyword(s):  
Crude Extract ◽  
Sperm Function ◽  
Mouse Sperm
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