A simple and sensitive catalytic method for determination of the total iron-binding capacity of blood serum

1979 ◽  
Vol 71 (3-4) ◽  
pp. 165-174 ◽  
Author(s):  
A. A. Alexiev ◽  
P. R. Bontchev ◽  
M. Khristova
Keyword(s):  
Blood Serum ◽  
Binding Capacity ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Catalytic Method ◽  
Iron Binding Capacity

scholarly journals Immunoturbidimetric determination of serum transferrin on a Kone Progress autoanalyser

1989 ◽  
Vol 11 (1) ◽  
pp. 40-41 ◽  
Author(s):  
Luc Cynober ◽  
Jacques Le Boucher ◽  
Jacqueline Giboudeau
Keyword(s):  
Binding Capacity ◽  
Clinical Chemistry ◽  
Total Iron ◽  
Radial Immunodiffusion ◽  
Serum Transferrin ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
French Society ◽  
Iron Binding Capacity

The Kone Progress, a multiparametric discrete analyser, was used to determine serum transferrin with a kit supplied by Kone. Assays recommended by the French Society of Clinical Chemistry were performed in order to assess the suitability of the test. Repeatability was assessed using serum pools with low (L), medium (M) and high (H) concentrations of transferrin. The coeffcients of variation (CV) were 5.4, 3.2 and 2.0% respectively for 30 determinations (within-batch). Reproducibility on 15 consecutive days (between-batch) was also satisfactory (CV for L = 7.3%, M = 6.3% and H = 3.8%). There was no serum-to-serum contamination. Results correlated closely with those obtained using radial immunodiffusion (RID) (r = 0.942) and total iron-binding capacity (r = 0.954)for 90 determinations.Transferrin measurement by immunoturbidimetry on the Kone Progress emerges as a well-suited, rapid and inexpensive alternative to other time-consuming (RID) and sophisticated (laser immunonephelemeter) techniques.

scholarly journals THE MAIN PARAMETERS OF IRON METABOLISM IN PATIENTS WITH UROTELIAL BLADDER CANCER AT DIFFERENT DEVELOPMENT STAGES OF MALIGNANT NEOPLASM ANEMIA

10.23856/4332 ◽  
2021 ◽  
Vol 43 (6) ◽  
pp. 247-255
Author(s):  
Dmytro Borysenko ◽  
Stanislav Vydyborets
Keyword(s):  
Bladder Cancer ◽  
Blood Serum ◽  
Binding Capacity ◽  
Malignant Neoplasm ◽  
Total Iron ◽  
The Body ◽  
Malignant Neoplasms ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Binding Capacity

The article presents information about the protein responsible for the iron depot in the body – ferritin. Its physiological role and clinical significance are demonstrated. A batophenanthroline method was used to determine the range of iron in the blood serum and the range of the total iron-binding capacity of the blood serum. The range of unsaturated iron-binding capacity of blood serum was calculated as the difference between total iron-binding capacity in blood serum and the range of iron in blood serum. The transferrin saturation coefficient of iron was defined as the ratio of the content of iron in blood serum to the total iron-binding capacity in blood serum. The range of transferrin was determined by the rate of the total iron-binding capacity in blood serum, and ferritin using the radiometric method. The dynamics of changes in ferritin content in malignant neoplasm anemia in patients with bladder cancer has been demonstrated. The conclusion about the importance of this parameter for laboratory diagnosis of iron deficiency and anemia of malignant neoplasms is made.

Sign in / Sign up

Export Citation Format

Share Document