Osmium tetroxide postfixation in relation to the crosslinkage and spatial organization of stereocilia in the guinea-pig cochlea

1985 ◽  
Vol 14 (1) ◽  
pp. 113-130 ◽  
Author(s):  
S. D. Comis ◽  
J. O. Pickles ◽  
M. P. Osborne
Keyword(s):  
Guinea Pig ◽  
Osmium Tetroxide ◽  
Spatial Organization ◽  
Guinea Pig Cochlea

Cell Reactivity Pattern of the Guinea Pig Cecal Mucosa Evidenced by the ZIO Technique

1982 ◽  
Vol 40 ◽  
pp. 50-51
Author(s):  
Juan Mora-Galindo ◽  
Jorge Arauz-Contreras
Keyword(s):  
Guinea Pig ◽  
Phase Contrast ◽  
Osmium Tetroxide ◽  
Cell Types ◽  
Cell Reactivity ◽  
Transmission Electron ◽  
Neural Tissues ◽  
Maturation Stages ◽  
Zinc Iodide ◽  
Cecal Mucosa

The zinc iodide-osmium tetroxide (ZIO) technique is presently employed to study both, neural and non neural tissues. Precipitates depends on cell types and possibly cell metabol ism as well.Guinea pig cecal mucosa, already known to be composed of epithelium with cells at different maturation stages and lamina propria which i s formed by morphologically and functionally heterogeneous cell population, was studied to determine the pat tern of ZIO impregnation. For this, adult Guinea pg cecal mucosa was fixed with buffered 1.2 5% g 1 utara 1 dehyde before incubation with ZIO for 16 hours, a t 4°C in the dark. Further steps involved a quick sample dehydration in graded ethanols, embedding in Epon 812 and sectioning to observe the unstained material under a phase contrast light microscope (LM) and a transmission electron microscope (TEM).

Ultrastructure of the lesion induced by toxin T-514 isolated from K. humboldtiana in the alveolar region of the lung

1992 ◽  
Vol 50 (1) ◽  
pp. 640-641
Author(s):  
Julio Sepúlveda-Saavedra ◽  
Beatriz González-Corona ◽  
Víctor A. Tamez Rodríguez ◽  
Ma. Victoria Bermúdez de Rocha ◽  
Alfredo Piñeyro López
Keyword(s):  
Electron Microscope ◽  
Guinea Pig ◽  
Macaca Fascicularis ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Severe Damage ◽  
Thin Sections ◽  
Alveolar Region ◽  
Histopathological Findings

It has been shown in previous studies that the toxin T-514 isolated from K. humboldtiana induces severe damage to the lung in treated rodents. Histopathological findings include edema, and alveolar hemorrage. However, the ultraestructure of the lesion has not been investigated. In this study we used two species of rodents: Hamster and guinea pig, and a primate: Macaca fascicularis. Animals received different single dosis of the toxin via intraperitoneal. Control animals received only the vehicle (propylen glycol). Inmediately after spontaneous death, lung samples were fixed in Karnovsky-Ito fixative, post fixed in osmium tetroxide and embedded in epon. Thin sections were prepared with an Ultratome V LKB, stained with uranly acetate and lead citrate, and studied in an electron microscope Zeiss-EM109.

Gentamicin alters Akt-expression and its activation in the guinea pig cochlea

Neuroscience ◽  
2015 ◽  
Vol 311 ◽  
pp. 490-498 ◽  
Author(s):  
U.-R. Heinrich ◽  
S. Strieth ◽  
I. Schmidtmann ◽  
H. Li ◽  
K. Helling
Keyword(s):  
Guinea Pig ◽  
Guinea Pig Cochlea

scholarly journals Effect of an ATP-Regulated Potassium Channel Opener, Nicorandil, on Isolated Outer Hair Cells of the Guinea Pig Cochlea

1998 ◽  
Vol 1998 (Supplement96) ◽  
pp. 23-30
Author(s):  
Akimitsu Kawai ◽  
Yukihiro Sato ◽  
Takeshi Akisada ◽  
Tsuyoshi Yoshihiro ◽  
Kotaro Take ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Potassium Channel ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Potassium Channel Opener ◽  
Guinea Pig Cochlea ◽  
Channel Opener
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