Thin-layer chromatography of isomeric monounsaturated fatty acids

1963 ◽  
Vol 12 (5) ◽  
pp. 869-869
Author(s):  
L. D. Bergel'son ◽  
�. V. Dyatlovitskaya ◽  
V. V. Voronkova
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Monounsaturated Fatty Acids ◽  
Layer Chromatography

Diacylglycerol Biosynthesis in Everted Sacs of Rat Intestinal Mucosa

1975 ◽  
Vol 53 (11) ◽  
pp. 1170-1183 ◽  
Author(s):  
W. C. Breckenridge ◽  
A. Kuksis
Keyword(s):  
Fatty Acids ◽  
Phosphatidic Acid ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Thin Layer Chromatography ◽  
Intestinal Mucosa ◽  
Gas Liquid Chromatography ◽  
Acid Pathway ◽  
Layer Chromatography ◽  
Everted Sacs

The molecular specificity in the biosynthesis of diacylglycerols by rat intestinal mucosa was examined by means of radioactive markers, thin-layer chromatography with silver nitrate and gas-liquid chromatography with radioactivity monitoring. Bile salt micelles of alternately labeled monoacylglycerols and free fatty acids were incubated with everted sacs of intestinal mucosa for various periods of time and the diacylglycerols were isolated by solvent extraction and thin-layer chromatography. Stereospecific analyses of the X-1,2-diacylglycerols labeled from 2-monoacylgiycerols showed that the sn-1,2-isomers (45–55%) were slightly in excess of the sn-2,3-isomers (34–45%) with the X-1,3-diacylglycerols accounting for the rest of the radioactivity (5–10%). This suggests that racemic diacylglycerols may be intermediates in the resynthesis of dietary fat in rat intestinal mucosa. Detailed analyses of the molecular species of the sn-1,2-diacylglycerols labeled from free fatty acids revealed that 10–45% of the total did not contain the acid present in the 2-monoacylglycerol supplied, and therefore had originated from the phosphatidic acid pathway. These findings are at variance with those obtained in isolated microsomes, which have suggested an inhibition of the phosphatidic acid pathway by monoacylglycerols as well as have given evidence of an exclusive synthesis of sn-1,2-diacylglycerols from 2-monoacylglycerols.

Mode of action of choline. III. Metabolism of nonessential fatty acids in choline-deficient rats

1969 ◽  
Vol 47 (6) ◽  
pp. 619-630 ◽  
Author(s):  
A. Chalvardjian
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Serum Triglycerides ◽  
Hepatic Lipids ◽  
Layer Chromatography ◽  
Serum Phospholipids ◽  
Early Alteration ◽  
Initial Change

To investigate the increase in ratio of C16 to C18 nonessential fatty acids in hepatic triglycerides of choline-deficient rats, two groups of rats fed, respectively, a choline-deficient and a choline-supplemented diet for 3–4 days were injected either with 1-14C-acetate intraperitoneally or with a mixture of 9,10-3H-palmitate and 18-14C-stearate intravenously. The choline-deficient and choline-supplemented rats were killed 3 h after labelled acetate injection. Further groups of choline-deficient and choline-supplemented rats were killed at intervals of 1 min to 6 h after injection with labelled palmitate and stearate. Extracts of lipids from livers and sera were analyzed by gas–liquid and thin-layer chromatography. In the choline-deficient rats injected with 1-14C-acetate the ratio of C16 to C18 labelled fatty acids incorporated into hepatic and serum triglycerides was increased and the ratio of those incorporated into hepatic and serum phospholipids was decreased. The ratio of monounsaturated fatty acids to saturated fatty acids incorporated into the triglycerides and phospholipids of liver and serum of the choline-deficient rats was decreased compared to that of the choline-supplemented rats. Similar differences between the two groups of rats were evident in the hepatic lipids of animals injected with 3H-palmitate and 14C-stearate. The early alteration of the ratios of hepatic nonessential fatty acids suggests that the initial change is a decreased desaturation of fatty acids.

Standardization of Homoeopathic Mother Tincture of Strychnous nux vomica with the help of High-Performance Thin Layer Chromatography and correlation of its alkaloid markers with its Toxicological action

2021 ◽  
pp. 4203-4206
Author(s):  
Dharmendra B. Sharma ◽  
Parth Aphale ◽  
Vineet Sinnarkar ◽  
Sohan S. Chitlange ◽  
Asha Thomas
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Therapeutic Action ◽  
Aluminium Sheets ◽  
Mother Tincture ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Dark Blue

Background: Chromatography is one of the important laboratory technique in which the components of a mixture are separated on an adsorbent in order to analyze, identify, purify and quantify a mixture. Thin Layer Chromatography (TLC)is used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound. High Performance Thin Layer Chromatography is a sophisticated and automated form of Thin Layer Chromatography (TLC). The procedure simultaneously processes the sample and standard that results in better analytical precision and accuracy at a faster pace. Pharmacological/ Toxicological action of Nux Vomica is because of its active principles present in the seeds namely strychnine, brucine etc. This research paper aims to corelate the active principles present in Nux Vomica with the toxicological action of the same. Materials and Methods: 1. Standard Nux Vomica mother tincture was tested for its alkaloid markers and its correlation with the toxicological action was studied. 2. Analysis of the mother tincture was done using High Performance Thin Layer Chromatography. 3. Stationary phase consisted of TLC Aluminium sheets with silica gel 60 F253 pre-coated layer (20cm x 10cm), thickness-0.2mm, no. of tracks-18, band length-6mm. 4. Mobile Phase consisted of Chloroform: Methanol (9.5:0.5). 5. The plate was developed in developing chamber and observed under U.V. Light. Results: Colours seen on the HPTLC Plates of samples are greenwhich corresponds to strychnine, dark blue which corresponds to brucine, orange to alkaloids fluorescent green to sterols and pink to fatty acids which are evident on the chromatogram. Conclusion: Therapeutic action of Nux Vomica as noted in Homoeopathic Materia Medica is because of the active principles like strychnine, brucine, alkaloids, sterols, fatty acids present in it which is evident from the chromatogram.

scholarly journals Recovery of Bioactive Compounds from Hazelnuts and Walnuts Shells: Quantitative–Qualitative Analysis and Chromatographic Purification

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1363 ◽  
Author(s):  
René Herrera ◽  
Jarl Hemming ◽  
Annika Smeds ◽  
Oihana Gordobil ◽  
Stefan Willför ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Antioxidant Capacity ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Short Chain Fatty Acids ◽  
Extraction Process ◽  
Size Exclusion ◽  
Flash Chromatography ◽  
Layer Chromatography

Hazelnut (HS) and walnut (WS) shells, an abundant by-product of the processing industries of these edible nuts, are traditionally considered as a low-value waste. However, they are a source of valuable compounds with an interesting chemical profile for the chemical and pharmaceutical sectors. In this study, the lipophilic and hydrophilic extracts present in HS and WS were quantified and identified, then the polar fractions were chromatographically separated, and their antioxidant capacity was studied. The experimental work includes the isolation of crude lipophilic and hydrophilic extracts by an accelerated extraction process, chromatographic analysis (gas chromatography-flame ionization (GC-FID), GC-mass spectroscopy (GC-MS), high-performance size-exclusion chromatography (HPSEC), thin-layer chromatography (TLC)), and quantification of the components. In addition, a thorough compositional characterization of the subgroups obtained by flash chromatography and their antioxidant capacity was carried out. The gravimetric concentrations showed different lipophilic/hydrophilic ratios (0.70 for HS and 0.23 for WS), indicating a higher proportion of polar compounds in WS than in HS. Moreover, the lipophilic extracts were principally composed of short-chain fatty acids (stearic, palmitic, and oleic acid), triglycerides, and sterols. The polar fractions were screened by thin-layer chromatography and then separated by flash chromatography, obtaining fractions free of fatty acids and sugar derivatives (97:3 in HS and 95:5 in WS), and mixtures richer in phenolic compounds and flavonoids such as guaiacyl derivatives, quercetin, pinobanksin, and catechin. The most polar fractions presented a higher antioxidant capacity than that of the crude extracts.

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