Transcription of chemically acetylated chromatin with homologous RNA polymerase B

1984 ◽  
Vol 4 (2) ◽  
pp. 155-163 ◽  
Author(s):  
A. Csordas ◽  
I. Multhaup ◽  
H. Grunicke
Keyword(s):  
Acetic Anhydride ◽  
Rna Polymerase ◽  
Rat Liver ◽  
Fold Increase ◽  
New Method ◽  
Liver Chromatin ◽  
High Degree

Homologous RNA polymerase B was used to examine the template properties of rat liver chromatin modified by acetic anhydride. Transcription of chromatin was strongly stimulated on the chemically acetyiated template. Under conditions of reinitiation inhibition there was an approximately two-fold increase in the number of initiation sites on the acetylated chromatin. A new method of chemical acetylation of histones, with a high degree of specificity, is presented.

scholarly journals Two species of RNA polymerase II released from rat liver chromatin

FEBS Letters ◽  
1979 ◽  
Vol 104 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Munehiko Yukioka ◽  
Koichiro Omori ◽  
Yasuji Okai ◽  
Akira Inoue
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rat Liver ◽  
Liver Chromatin

scholarly journals Location of endogenous RNA polymerase B in a sub-fraction of rat liver chromatin

FEBS Letters ◽  
1976 ◽  
Vol 61 (2) ◽  
pp. 166-170 ◽  
Author(s):  
Etienne Pays ◽  
Jacqueline Flamand
Keyword(s):  
Rna Polymerase ◽  
Rat Liver ◽  
Liver Chromatin

scholarly journals Characterization of double-stranded ribonucleic acid sequences present in the initial transcription products of rat liver chromatin

1977 ◽  
Vol 165 (2) ◽  
pp. 237-245 ◽  
Author(s):  
E Pays
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rat Liver ◽  
Rna Sequences ◽  
Complementary Sequences ◽  
Exogenous Rna ◽  
Liver Chromatin ◽  
Low Ionic Strength

At low ionic strength and with a low exogenous RNA polymerase/DNA ratio, rat liver chromatin directs the synthesis in vitro of RNA sequences rich in double-stranded segments. All the transcripts contain at least one double-stranded sequence. Most of the double-stranded segments are formed by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. They are heterogeneous in size, 35-45% of them being more than 80 nucleotides long. They contain 61-64% G+C, whether synthesized by rat liver RNA polymerase (form B) or Escherichia coli RNA polymerase. The largest double-stranded sequences are found in the largest transcripts, and are the most thermostable. The fidelity of base-matching is better in double-stranded transcripts synthesized on rat liver chromatin by homologous polymerase than in those synthesized on it by a bacterial polymerase, or in those synthesized by either of the two polymerases on pure DNA.

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