Shape transformation of erythrocyte ghosts depends on ion concentrations

1985 ◽  
Vol 5 (5) ◽  
pp. 417-423 ◽  
Author(s):  
Andreas Herrmann ◽  
Peter Müller ◽  
Roland Glaser
Keyword(s):  
Human Erythrocyte ◽  
Transmembrane Potential ◽  
Human Erythrocytes ◽  
Erythrocyte Ghosts ◽  
Shape Transformation ◽  
Ion Concentrations ◽  
Nacl Concentration ◽  
Shape Transformations

Resealed human erythrocyte ghosts undergo shape transformations similar to those of intact erythrocytes. The results indicate that the shape of these ghosts depends on the inner as well as on the outer NaCl concentration. A correlation between shape and calculated transmembrane potential was established similar to that for intact human erythrocytes.

Lead and calcium transport in human erythrocyte

1999 ◽  
Vol 18 (5) ◽  
pp. 327-332 ◽  
Author(s):  
J V Calderón-Salinas ◽  
M A Quintanar-Escorcia ◽  
M T González-Martínez ◽  
C E Hernández-Luna
Keyword(s):  
Transport System ◽  
Human Erythrocyte ◽  
Calcium Transport ◽  
Human Erythrocytes ◽  
The Other ◽  
Erythrocyte Ghosts ◽  
Passive Transport ◽  
Ca Uptake

In this paper we report the lead (Pb) and calcium (Ca) uptake by erythrocyte ghosts. In both cases the transport was carried out by a passive transport system with two kinetic components (Michaelis-Menten and Hill). Pb and Ca were capable of inhibiting the transport of the other metal in a non-competitive way. Under hyperpolarization, the uptakes of Ca and Pb were enhanced and the Michaelis-Menten component prevailed. Both Ca and Pb uptakes were inhibited by N-ethyl-maleimide to the same extent. These results indicate that Pb and Ca share the same permeability pathway in human erythrocytes and that this transport system is electrogenic.

The influence of chlorpromazine on the potential-induced shape change of human erythrocyte

1991 ◽  
Vol 11 (4) ◽  
pp. 213-221 ◽  
Author(s):  
J. Hartmann ◽  
R. Glaser
Keyword(s):  
Red Blood Cells ◽  
Human Erythrocyte ◽  
Blood Cells ◽  
Characteristic Time ◽  
Time Course ◽  
Transmembrane Potential ◽  
Shape Change ◽  
Human Erythrocytes

The effect of chlorpromazine (CPZ) on the shape of human erythrocytes with different values of transmembrane potential (TMP) was investigated. The shape of red blood cells with negative values of the TMP remained unchanged after the formation of stomatocytes by chlorpromazine, while cells with positive TMP showed a characteristic time course of shape change during the incubation with CPZ. Experiments with vanadate show that this might be due to a difference in the activity of the phospholipid-translocase at different values of TMP.

Further evidence for a membrane potential-dependent shape transformation of the human erythrocyte membrane

1986 ◽  
Vol 6 (11) ◽  
pp. 999-1006 ◽  
Author(s):  
Peter Müller ◽  
Andreas Herrmann ◽  
Roland Glaser
Keyword(s):  
Membrane Potential ◽  
Ionic Strength ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Transmembrane Potential ◽  
Sodium Gluconate ◽  
Human Erythrocyte Membrane ◽  
Shape Transformation

The influence of various factors (pH, temperature, sodium gluconate) on the ionic strength-dependent stomatocyte-discocyte-echinocyte transformation of the human erythrocyte membrane was investigated. The results give further evidence for a correlation between shape of erythrocyte membrane and the transmembrane potential of the cells.

scholarly journals FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS

1974 ◽  
Vol 63 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Hava Peretz ◽  
Zivia Toister ◽  
Yehudith Laster ◽  
Abraham Loyter
Keyword(s):  
Red Blood Cells ◽  
Clostridium Perfringens ◽  
Human Erythrocyte ◽  
Blood Cells ◽  
Sendai Virus ◽  
Iodoacetic Acid ◽  
Human Erythrocytes ◽  
Erythrocyte Ghosts ◽  
Human Red Blood Cells ◽  
Bivalent Cations

Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepared by gradual hemolysis still retain the potential to undergo virus-induced fusion. Fusion of human red blood cells without the addition of viruses was obtained by incubation of erythrocytes at pH 10.5 in the presence of Ca++ (40 mM) or by addition of phospholipase C Clostridium perfringens preparations to cells previously agglutinated or polylysine.

ATP Dependent Uptake of Zinc by Human Erythrocyte Ghosts

1978 ◽  
Vol 33 (3-4) ◽  
pp. 210-215 ◽  
Author(s):  
Georg Schmetterer
Keyword(s):  
Atomic Absorption ◽  
Energy Dependence ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Human Erythrocyte ◽  
Human Erythrocytes ◽  
Erythrocyte Ghosts ◽  
Passive Mechanism ◽  
Resealed Ghosts

The energy dependence of Zn2+ accumulation by human erythrocytes was studied by preparing resealed ghosts loaded with ATP or AMP. Uptake of Zn2+ was followed by radioactivity or atomic absorption spectroscopy measurements. Zn2+ taken up is not adsorbed by the mem­brane but transported into the ghosts. Ghosts containing ATP took up more Zn2+ than those con­taining AMP. The effect is not specific for ATP, however, GTP and CTP showing the same in­creased uptake. Zn2+ uptake by ATP ghosts can be further drastically enhanced by addition of Cu2t. Therefore, in spite of the enhancement of Zn2+ uptake by ATP, Zn2+ seems to be accumulated by a passive mechanism even when the ghosts contain ATP. The accumulation is probably brought about by binding of Zn2+ to substances inside the ghosts.

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