Monoclonal antibodies to the two most basic papaya proteinases

1986 ◽  
Vol 6 (8) ◽  
pp. 759-766 ◽  
Author(s):  
Peter W. Goodenough ◽  
Peter J. Kilshaw ◽  
Fiona McEwan ◽  
A. Jane Owen
Keyword(s):  
Monoclonal Antibodies ◽  
Structural Feature ◽  
Carica Papaya ◽  
Present Report ◽  
Proteinase A ◽  
Common Structural Feature

The proteinases from Carica papaya include papain, isoenzymes of chymopapain and two proteinases A and B distinguished by their unusually high pI. The identity of one of the most basic proteinases has been questioned. The present report describes the preparation and characterisation of two monoclonal antibodies that react specifically with papaya proteinases A and B respectively and a third that identifies a common structural feature found in papain and proteinase A.

scholarly journals Absolute Stereochemistry of the β-Hydroxy Acid Unit in Hantupeptins and Trungapeptins

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Deepak Kumar Gupta ◽  
Gary Chi Ying Ding ◽  
Yong Chua Teo ◽  
Lik Tong Tan
Keyword(s):  
Amino Acid ◽  
Structural Feature ◽  
Absolute Configuration ◽  
Hydroxy Acid ◽  
Hplc Analysis ◽  
The Absolute ◽  
Absolute Stereochemistry ◽  
Common Structural Feature ◽  
Acid Unit

The β-hydroxy/amino acid unit is a common structural feature of many bioactive marine cyanobacterial depsipeptides. In this study, the absolute stereochemistry of the β-hydroxy acid moieties in hantupeptins and trungapeptins were determined through their synthesis and HPLC analysis of the Mosher ester derivatives. Synthesis of two3-hydroxy-2-methyloctanoic acid (Hmoa) stereoisomers, (2 S,3 R)-Hmoa and (2 S,3 S)-Hmoa, were achieved using diastereoselective asymmetric method and the retention times of all four Hmoa isomers were established indirectly by RPLC-MS analysis of their Mosher ester derivative standards. Based on the retention times of the standards, the absolute configuration of the Hmoa unit in hantupeptin C (3) and trungapeptin C (6) was assigned as (2 R,3 S)- and (2 S,3 R)-Hmoa, respectively. The use of the Mosher's reagents, coupled with HPLC analysis, provided a viable alternative to the absolute stereochemical determination of β-hydroxy acid units in depsipeptides.

scholarly journals Identification of a phosphoprotein in the nuclear matrix by monoclonal antibodies

1987 ◽  
Vol 241 (3) ◽  
pp. 693-697 ◽  
Author(s):  
M J Halikowski ◽  
C C Liew
Keyword(s):  
Monoclonal Antibodies ◽  
Nuclear Matrix ◽  
Matrix Protein ◽  
Solid Phase ◽  
Present Report ◽  
Nuclear Organization ◽  
Micrococcal Nuclease ◽  
Nuclear Matrix Protein ◽  
Solid Phase Radioimmunoassay ◽  
Nuclear Phosphoprotein

Previous work in this laboratory has established that a rat liver nuclear phosphoprotein (B2:Mr 68,000, pI 6.5-8.2) is associated with actively transcribed nucleosomes, as demonstrated by its preferential release after mild treatment with micrococcal nuclease. In the present report we provide further immunological evidence (‘Western Blot’ analysis, solid-phase radioimmunoassay and indirect immunofluorescence) that in addition establishes the presence of this phosphoprotein in the nuclear-matrix protein fraction. This paradoxical localization suggests that this phosphoprotein may function in two separate and distinct roles within the realm of nuclear organization.

Comparison of transfer RNA and ribosomal RNA intron splicing in the extreme thermophile and archaebacterium Desulfurococcus mobilis

1989 ◽  
Vol 35 (1) ◽  
pp. 210-214 ◽  
Author(s):  
Jørgen Kjems ◽  
Jonna Jensen ◽  
Tina Olesen ◽  
Roger A. Garrett
Keyword(s):  
Structural Feature ◽  
Ribosomal Rna ◽  
Structural Features ◽  
Intron Evolution ◽  
Extreme Thermophile ◽  
Cleavage Process ◽  
Cleavage Enzyme ◽  
Intron Boundary ◽  
23S Ribosomal Rna ◽  
Common Structural Feature

The structure of the exon–intron boundary was compared for an intron within 23S ribosomal RNA of Desulfurococcus mobilis and a newly discovered intron in tRNAMet from the same organism. The occurrence of a putative common structural feature suggests that intron excision occurs by the same mechanism. The possible recognition of this structural feature by the cleavage enzyme was investigated for the ribosomal RNA intron using RNA substrates exhibiting various exon and intron deletions. The results support the involvement of the structural features in the cleavage process. The evolutionary implications of these results are considered.Key words: archaebacteria, tRNA, ribosomal RNA, introns, intron evolution.

scholarly journals Use of precision profiles to evaluate precision of the automated leukocyte differential

1996 ◽  
Vol 42 (7) ◽  
pp. 1068-1073 ◽  
Author(s):  
W Hübl ◽  
L Tlustos ◽  
P M Bayer
Keyword(s):  
Monoclonal Antibodies ◽  
Leukocyte Count ◽  
Present Report ◽  
Statistical Significance ◽  
Fixed Number ◽  
Fixed Amount ◽  
Flow Cytometric ◽  
Instruments And Techniques ◽  
Comparability Of Results ◽  
Hematology Analyzers

Abstract The commonly used methods of assessing the precision of the automated leukocyte differential have certain drawbacks that affect the validity and comparability of results. In the present report, we introduce a procedure based on building precision profiles from a large number of within-run imprecision experiments. The profiles are fitted to the function for the CV of proportions, which yields the number of theoretically differentiated leukocytes. Differences between fitted curves are evaluated for statistical significance by the F-test. As an example, we compared the precision of two hematology analyzers, a flow-cytometric technique involving fluorescence-labeled monoclonal antibodies, and the manual differential. We were able to establish definite differences in precision between different analyzers and different leukocyte classes. Our data also indicated that conventional within-run imprecision studies may completely misjudge analyzer precision. Furthermore, we could demonstrate that the precision of analyzers that analyze a fixed amount of blood rather than a fixed number of leukocytes is strongly influenced by the leukocyte count of the sample, leading to high imprecision for leukopenic samples. We believe the proposed procedure is a useful addition to currently used protocols; it yields clear results and creates a statistical basis of comparison between various instruments and techniques of differentiation.

scholarly journals A common structural feature in promoter sequences ofE. coli

1987 ◽  
Vol 15 (12) ◽  
pp. 4973-4985 ◽  
Author(s):  
Chang-Shung Tung ◽  
Stephen C. Harvey
Keyword(s):  
Structural Feature ◽  
Promoter Sequences ◽  
Common Structural Feature

scholarly journals Identification of benzopyrone as a common structural feature in compounds with anti-inflammatory activity in a zebrafish phenotypic screen

2016 ◽  
Vol 9 (6) ◽  
pp. 621-632 ◽  
Author(s):  
Anne L. Robertson ◽  
Nikolay V. Ogryzko ◽  
Katherine M. Henry ◽  
Catherine A. Loynes ◽  
Matthew J. Foulkes ◽  
...  
Keyword(s):  
Structural Feature ◽  
Inflammatory Activity ◽  
Phenotypic Screen ◽  
Anti Inflammatory ◽  
Common Structural Feature

scholarly journals The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 456-463
Author(s):  
ED Ball ◽  
MW Fanger
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Myeloid Leukemia ◽  
Present Report ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Specific Cell ◽  
Normal Peripheral Blood ◽  
Myelocytic Leukemia ◽  
Specific Cell Surface

The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

scholarly journals The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 456-463 ◽  
Author(s):  
ED Ball ◽  
MW Fanger
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Myeloid Leukemia ◽  
Present Report ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Specific Cell ◽  
Normal Peripheral Blood ◽  
Myelocytic Leukemia ◽  
Specific Cell Surface

Abstract The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

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