The influence of post-filtration washing on the in vitro assay ofCandida albicans adherence to human buccal epithelial cells

1993 ◽  
Vol 121 (1) ◽  
pp. 11-15
Author(s):  
Elizabeth D. Theaker ◽  
D. B. Drucker ◽  
A. C. C. Gibbs
Keyword(s):  
Epithelial Cells ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Ofcandida Albicans ◽  
Buccal Epithelial Cells

An adapted in vitro assay to assess Campylobacter jejuni interaction with intestinal epithelial cells: Taking into stimulation with TNFα

2018 ◽  
Vol 149 ◽  
pp. 67-72 ◽  
Author(s):  
Ramila Cristiane Rodrigues ◽  
Anne-Lise Pocheron ◽  
Jean-Michel Cappelier ◽  
Odile Tresse ◽  
Nabila Haddad
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Intestinal Epithelial Cells ◽  
In Vitro Assay ◽  
Intestinal Epithelial ◽  
Vitro Assay

A simple quantitative in vitro assay for thymocyte adhesion to thymic epithelial cells using a fluorescein diacetate

1993 ◽  
Vol 157 (1-2) ◽  
pp. 117-123 ◽  
Author(s):  
Yasuyuki Maeda ◽  
Kazuo Tanaka ◽  
Yasuhiro Koga ◽  
Xin-Ying Zhang ◽  
Masafumi Sasaki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Assay ◽  
Fluorescein Diacetate ◽  
Thymic Epithelial Cells ◽  
Vitro Assay

Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants

2005 ◽  
Vol 71 (1) ◽  
pp. 61-72 ◽  
Author(s):  
Bingsheng Zhou ◽  
Wenhua Liu ◽  
Rudolf S.S. Wu ◽  
Paul K.S. Lam
Keyword(s):  
Epithelial Cells ◽  
Oreochromis Niloticus ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Synthesis and Biological Evaluation of Haloalkyl Phosphate Triester Derivatives of araA and araC

1992 ◽  
Vol 3 (2) ◽  
pp. 79-84 ◽  
Author(s):  
C. McGuigan ◽  
B. C. N. M. Jones ◽  
S. M. Tollerfield ◽  
P. A. Riley
Keyword(s):  
Epithelial Cells ◽  
Antiviral Drug ◽  
Biological Evaluation ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Free Nucleotides ◽  
Synthesis And Biological Evaluation ◽  
Derivatives Of

Novel phosphate triester derivatives of the antiviral drug araA and the anti-leukaemic agent araC have been prepared as membrane-soluble pro-drugs of the bio-active free nucleotides. In particular, novel trichloro- and trifluoroethyl phosphates have been prepared using phosphorochloridate chemistry, and are fully characterized. An in vitro assay indicates inhibition, by each of the compounds, of thymidine incor-portion by mammalian epithelial cells. It is notable that the trichloroethyl derivative is most active in each case, and in the case of araC its activity appears to exceed that of the parent nucleoside.

In vitro binding of Candida albicans yeast cells to human fibronectin

1984 ◽  
Vol 30 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Katherine G. Skerl ◽  
Richard A. Calderone ◽  
Esther Segal ◽  
T. Sreevalsan ◽  
W. Michael Scheld
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Mammalian Cells ◽  
Fluorescent Antibody ◽  
In Vitro Assay ◽  
Yeast Cells ◽  
Vitro Assay ◽  
Vaginal Epithelial Cells ◽  
Human Fibronectin

The binding of Candida albicans yeast cells to human fibronectin (Fn), a major glycoprotein of mammalian cells, was studied using an in vitro assay. Adherence was quantitated in microtiter dishes coated with Fn to which radiolabeled yeast cells were added. Under optimum conditions of the assay, i.e., 1 mM CaCl2 and 70 μg Fn protein, approximately 40% of the radiolabeled yeast cells adhered to the Fn. Adherence to Fn was greater at 30 °C than at 4 °C and was greater with viable yeast cells than with heat-killed cells. Candida albicans (two strains) and C. tropicalis adhered to Fn to a greater extent than C. pseudotropicalis, C. krusei, or Saccharomyces cerevisiae. Pretreatment of C. albicans with chymotrypsin, pronase, or papain, but not pepsin, decreased adherence to Fn. Blocking experiments using mannan, sugars, or amino sugars were carried out by preabsorbing the Fn with each of the above-mentioned compounds. Candida mannan blocked adherence of C. albicans to Fn. The mannan effect was dose dependent. However, adherence of C. albicans to Fn was not significantly reduced by mannose, glucose, or several other sugars. The role of FN as a receptor for the binding of C. albicans yeast cells to buccal and vaginal epithelial cells was investigated also using an in vitro assay. We determined, using indirect fluorescent antibody techniques, that both buccal and vaginal epithelial cells possessed Fn. In addition, yeast cells, when pretreated with Fn, showed reduced adherence with buccal and vaginal cells when compared with nontreated cells. These studies may indicate a role for Fn in the adherence of C. albicans to buccal and vaginal epithelial cells.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Angiogenic Effect of Pravastatin Alone and with Sera from Healthy and Complicated Pregnancies Studied by in vitro Vasculogenesis/Angiogenesis Assay

2021 ◽  
pp. 1-9
Author(s):  
Anita Virtanen ◽  
Outi Huttala ◽  
Kati Tihtonen ◽  
Tarja Toimela ◽  
Tuula Heinonen ◽  
...  
Keyword(s):  
Direct Effect ◽  
Late Onset ◽  
Maternal Serum ◽  
In Vitro Assay ◽  
Serum Samples ◽  
Therapeutic Concentration ◽  
Tubule Formation ◽  
Vitro Assay ◽  
Angiogenesis Assay

<b><i>Objective:</i></b> To determine the direct effect of pravastatin on angiogenesis and to study the interaction between pravastatin and maternal sera from women with early- or late-onset pre-eclampsia (PE), intrauterine growth restriction, or healthy pregnancy. <b><i>Methods:</i></b> We collected 5 maternal serum samples from each group. The effect of pravastatin on angiogenesis was assessed with and without maternal sera by quantifying tubule formation in a human-based in vitro assay. Pravastatin was added at 20, 1,000, and 8,000 ng/mL concentrations. Concentrations of angiogenic and inflammatory biomarkers in serum and in test medium after supplementation of serum alone and with pravastatin (1,000 ng/mL) were measured. <b><i>Results:</i></b> Therapeutic concentration of pravastatin (20 ng/mL) did not have significant direct effect on angiogenesis, but the highest concentrations inhibited angiogenesis. Pravastatin did not change the levels of biomarkers in the test media. There were no changes in angiogenesis when therapeutic dose of pravastatin was added with maternal sera, but there was a trend to wide individual variation towards enhanced angiogenesis, particularly in the early-onset PE group. <b><i>Conclusions:</i></b> At therapeutic concentration, pravastatin alone or with maternal sera has no significant effect on angiogenesis, but at high concentrations the effect seems to be anti-angiogenic estimated by in vitro assay.

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