The relationship of transmitter release and storage to fine structure in a sympathetic ganglion

1974 ◽  
Vol 3 (2) ◽  
pp. 133-160 ◽  
Author(s):  
R. I. Birks
1964 ◽  
Vol 23 (1) ◽  
pp. 63-78 ◽  
Author(s):  
James R. Coleman ◽  
Montrose J. Moses

The indium trichloride method of Watson and Aldridge (38) for staining nucleic acids for electron microscopy was employed to study the relationship of DNA to the structure of the synaptinemal complex in meiotic prophase chromosomes of the domestic rooster. The selectivity of the method was demonstrated in untreated and DNase-digested testis material by comparing the distribution of indium staining in the electron microscope to Feulgen staining and ultraviolet absorption in thicker sections seen with the light microscope. Following staining by indium, DNA was found mainly in the microfibril component of the synaptinemal complex. When DNA was known to have been removed from aldehyde-fixed material by digestion with DNase, indium stainability was also lost. However, staining of the digested material with non-selective heavy metal techniques demonstrated the presence of material other than DNA in the microfibrils and showed that little alteration in appearance of the chromosome resulted from DNA removal. The two dense lateral axial elements of the synaptinemal complex, but not the central one to any extent, also contained DNA, together with non-DNA material.


2021 ◽  
Vol 27 (3) ◽  
pp. 167-171
Author(s):  
Shareef Muhammed ◽  
Chitra Rajeswary ◽  
Anil Chandran

Eugenia roxburghii is an evergreen graceful shrub with a tremendous potential as garden plant. As a part of ex-situ conservation and popularization of the species, seed longevity was studied by understanding the relationship of seed viability with respect to different moisture contents and storage temperature. Seeds are recognized as recalcitrant, being desiccation as well as chilling sensitive. During hermetic storage, seeds stored at 300C/70%RH retained viability for about 5 months and 4 months in 200C/20% RH. Seeds can be best stored for five months in laboratory conditions.


2016 ◽  
Vol 23 (2) ◽  
pp. 73-78
Author(s):  
T. Sabu ◽  
P.S. Shameer ◽  
Chitra Rajeswary ◽  
N. Mohanan ◽  
C. Anilkumar

Seeds of Garcinia talbotii remained viable hardly for two weeks in open room conditions. As part of ex-situ conservation of Garcinia talbotii, seeds longevity was studied since seeds are the main propagule. For this, the relationship of seed viability with respect to different moisture content and storage temperature were analyzed. Seed storage behaviour is also investigated. Being recalcitrant, seeds are desiccation sensitive as well as chilling sensitive. During hermetic storage of seeds at 300C /70 % RH seeds retained viability about 6 months.


1970 ◽  
Vol 53 (6) ◽  
pp. 1120-1128
Author(s):  
T Fazio ◽  
J W Howard ◽  
E O Haenni

Abstract A national survey was conducted to ascertain the relationship of the cyclohexylamine content of cyclamate-containing products to their composition, processing, and storage conditions. Cyclohexylamine was found in 174 of the 232 samples examined. The cyclohexylamine content range for each major type of food product was as follows: 0.0–8.2 ppm for carbonated beverages; 0.0–5.8 ppm for dry beverage bases; 0.0–1.5 ppm for fruit juices; 0.0–0.8 ppm for weight control foods; and 0.3–66 ppm for food sweetener preparations (liquid and dry base). No correlation between the cyclamate content of products and the cyclohexylamine present was evident. However, the findings indicate that significant hydrolysis of cyclamate can occur.


1976 ◽  
Vol 54 (6) ◽  
pp. 843-851 ◽  
Author(s):  
John H. Youson

Granulated cells located in the walls of the posterior cardinal and renal veins in the holostean fish Amia calva resemble cells of the adrenal medulla of higher vertebrates. The cells all contain similar electron-dense secretory granules, apparently originating from the Golgi apparatus, and are innervated by nerve terminals. These features suggest that the cells are involved in the production and release of catecholamines. The relationship of the cells to the endothelium of the large veins also suggests that the secretory products of the cells reach the circulation by traversing a complex subendothelial space and passing through the fenestrae of the endothelium. This appears to be an efficient means for the immediate release and distribution of catecholamines in this species of fish.


2017 ◽  
Vol 142 (2) ◽  
pp. 92-100 ◽  
Author(s):  
Xingbin Xie ◽  
Congbing Fang ◽  
Yan Wang

Bosc is a winter cultivar of european pear (Pyrus communis) that has a relatively short storage life partially due to a high ethylene production rate (EPR) during cold storage. ‘Bosc’ pears were harvested at commercial maturity and treated with gas 1-methylcyclopropene (1-MCP) at 0, 0.15, and 0.3 µL·L−1 and stored at −1.1 °C for 8 months. Results indicated that all 1-MCP treatments inhibited EPR and respiration rate (RR), retarded the degradation of chlorophyll and titratable acidity (TA), and extended storage quality; but inhibited ripening capacity. 1-MCP at 0.15 and 0.3 µL·L−1 had the same efficacy on keeping fruit quality although its higher rate was more efficient on inhibiting EPR and RR. The expression of ethylene synthesis genes (PcACS1, PcACS2, PcACS4, PcACS5, and PcACO1) and receptor genes (PcETR1, PcETR2, and PcERS1) was upregulated in control fruit during storage and they were downregulated significantly by 1-MCP treatments. In contrast, the ethylene receptor genes of PcETR5 and PcCTR1 were downregulated in control fruit during storage and were unaffected by 1-MCP treatments. Although the transcription levels of chlorophyll degradation genes PcPPH, PcNOL, PcSGR, PcRCCR, PcNYC, and PcPAO were all upregulated in control fruit during storage and downregulated by 1-MCP; only PcCHL was downregulated in the control and 1-MCP had no consistent effect on it. The relationship of ethylene biosynthesis/perception with chlorophyll degradation and storage quality in european pears was discussed.


Blood ◽  
1965 ◽  
Vol 25 (3) ◽  
pp. 345-355 ◽  
Author(s):  
BARRY G. FIRKIN ◽  
BARRY J. O’NEILL ◽  
BETH DUNSTAN ◽  
RONALD OLDFIELD

Abstract The electron microscopic appearance of platelets incubated at 37 C. and stored at 4 C. are described, and the relationship of these changes to normal platelet structure are discussed together with the hypothesis that the observation may be due to lysosome activity of some of the platelet granules. The platelet’s ATP level during incubation and storage was also followed and it was concluded that this relates more to the platelet’s ability to sustain clot retraction than to its viability.


2022 ◽  
Vol 2159 (1) ◽  
pp. 012009
Author(s):  
J E Camargo-Chávez ◽  
S Arceo-Díaz ◽  
E E Bricio-Barrios ◽  
R E Chávez-Valdez

Abstract Emerging technologies are efficient alternatives for satisfying the growing demand for sustainable and cheap energy sources. Piezoelectrics are one of the most promising energy sources derived from emerging technologies. These materials are capable of converting mechanical energy into electricity or vice versa. Piezoelectrics have been used for almost a hundred years to generate electrical and sound pulses. However, the use of piezoelectrics for power generation is constrained by the cost associated with equipment and infrastructure. This problem has been addressed through mathematical models that relate the physical and electrical properties of the piezoelectric material with the voltage generated. Although these models have high performance, they do not incorporate voltage rectification and electrical charge storage stages. This work presents a mathematical model that describes the relationship of the physical and electromechanical properties of a system employing a piezoelectric for energy generation. The voltage of the system and the charge stored in a capacitor are calculated through this model. Also, contour diagrams are presented as a tool for facilitating the efficiency of energy generation.


Blood ◽  
1970 ◽  
Vol 35 (4) ◽  
pp. 437-446 ◽  
Author(s):  
JAMES G. WHITE

Abstract The tendency of polymers of sickled hemoglobin (HbS) to align parallel and equidistant to each other has been pointed out in many investigations, and is considered a characteristic feature of erythrocyte sickling. A previous study on stroma-free solutions of sickled hemoglobin, however, suggested that polymers of HbS preferentially assumed radial rather than parallel relationships. Sickled erythrocytes were exposed to hypertonic stress in the present study in order to observe whether parallel bundles of polymers remained intact after removal of the cell membrane. Bundles of polymers in salicylate damaged sickled cells regularly developed branching and radial configurations similar to those found in stroma-free gels. Rotational stress appears to be the dominant factor influencing the relationship of HbS polymers, and the force generated by that tension may be an important factor in erythrocyte sickling.


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