Amino acid composition of some Swedish cultivars of Brassica species determined by gas liquid chromatography

1977 ◽  
Vol 27 (3-4) ◽  
pp. 255-263 ◽  
Author(s):  
L. -A. Appelqvist ◽  
Baboo M. Nair
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Brassica Species ◽  
Gas Liquid Chromatography

scholarly journals Features of сalibration еequations for IR scanners in determining the amino acid composition of soy proteins

2020 ◽  
Author(s):  
С.Е. НИЗКИЙ ◽  
Г.А. КОДИРОВА ◽  
Г.В. КУБАНКОВА
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
High Performance ◽  
Near Infrared ◽  
Soybean Proteins ◽  
Calibration Equation ◽  
Calibration Equations

Из 20 аминокислот, входящих в состав растительных белков, 17 лучше всего определяются с помощью высокоэффективной жидкостной хроматографии. Но эта технология затратна по времени, в том числе из-за подготовки проб, что делает ее малопригодной при проведении массовых анализов, например при оценке селекционного материала. В этом случае наиболее приемлемы технологии, основанные на сканировании в ближнем инфракрасном диапазоне излучения. Несмотря на то что ИК-сканеры способны по одному калибровочному уравнению выявлять большое количество компонентов, необходима постоянная коррекция при определении состава аминокислот и приведении его в процентное соотношение. В статье рассматриваются варианты создания калибровочных уравнений для расчета аминокислотного состава белков сои с помощью компьютерных программ (Nir 42, ISI), обеспечивающих работу ИК-сканеров типа NIR-4250 или FOSS NIRSystem 5000. Установлено, что при создании калибровочных уравнений содержание каждой аминокислоты наиболее корректно выражать в абсолютных единицах (г на 100 г белка), а не относительных (%). 17 of the 20 amino acids, included in the composition of plant proteins, are most effectively determined using liquid chromatography. The technology of high-performance liquid chromatography is to a certain extent costly in time, among other things because of sample preparation that makes it unsuitable for mass analysis, for example, when evaluating a breeding material. In this case, the technology based on scanning in the near infrared radiation band are the most acceptable. Despite the fact that IR scanners are able to determine a sufficiently large number of components on the basis of one calibration equation, a constant correction is required when determining the composition of amino acids and reducing it to a percentage ratio. The options for creating calibration equations for determining the amino acid composition of soybean proteins for computer programs (Nir 42, ISI), which provide the operation of IR scanners, such as NIR-4250 or FOSS NIRSystem 5000 are considered in the article. It was found that when creating calibration equations, it is most correct to set for each amino acid its mass content (g per 100 g of protein), and not the relative portion (in %).

scholarly journals Determination of Amino Acid Composition in the Harpagophytum procumbens Root

2019 ◽  
Vol 18 (1) ◽  
pp. 85-91
Author(s):  
AI Kriukova ◽  
IM Vladymyrova ◽  
OL Levashova ◽  
TS Tishakova
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
High Performance ◽  
Harpagophytum Procumbens

The amino acid composition of the roots of Harpagophytum procumbens was investigated by the method of high performance liquid chromatography (HPLC) with preliminary derivatization. Sixteen free and thirteen bound amino acids were quantitatively determined. The content of protein-bound amino acids was calculated. Dhaka Univ. J. Pharm. Sci. 18(1): 85-91, 2019 (June)

scholarly journals Amino acid identification of Bombyx mori fibroin cocoon as biomaterial using liquid chromatography/mass spectrometry

2020 ◽  
Vol 28 ◽  
pp. 02001
Author(s):  
Sartika Puspita ◽  
Siti Sunarintyas ◽  
Chairil Anwar ◽  
Ema Mulyawati ◽  
Marsetyawan HNE Soesatyo
Keyword(s):  
Mass Spectrometry ◽  
Mechanical Properties ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Bombyx Mori ◽  
Acid Composition ◽  
Liquid Chromatography Mass Spectrometry ◽  
Central Java ◽  
Chromatography Mass Spectrometry

A fibroin is a natural polymer that serves as a biomaterial because of the mechanical properties, biocompatible, biodegradable and amino acid composition. The mechanical properties of fibroin are influenced by crystalline structures of amino acid and are related to the amino acid composition. it can accelerate the wound healing because it promotes the proliferation of exposure tissue and inhibits inflammation. The study aimed to identify amino acid of Bombyx mori (B. mori) fibroin cocoon as biomaterial that was cultivated in Central Java Indonesia. Liquid Chromatography/Mass Spectrometry (LC/MS-Water, USA) was employed. This study found that fibroin of B. mori contains seventeen amino acids and the most abundant amino acid is L-Valin 4.81 % (mg kg–1).

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