The histochemical demonstration of glyceraldehyde phosphate dehydrogenase activity with a semipermeable membrane technique

1980 ◽  
Vol 12 (1) ◽  
pp. 119-122 ◽  
Author(s):  
G. P. de Vries ◽  
A. J. Tigges ◽  
A. E. F. H. Meijer
Keyword(s):  
Dehydrogenase Activity ◽  
Histochemical Demonstration ◽  
Semipermeable Membrane ◽  
Glyceraldehyde Phosphate Dehydrogenase ◽  
Membrane Technique

scholarly journals Enhanced resolution of histochemical distribution of glucose-6-phosphate dehydrogenase activity in rat neural tissue by use of a semipermeable membrane.

1991 ◽  
Vol 39 (7) ◽  
pp. 937-943 ◽  
Author(s):  
M A Philbert ◽  
C M Beiswanger ◽  
T L Roscoe ◽  
D K Waters ◽  
H E Lowndes
Keyword(s):  
Vestibular Nucleus ◽  
Neural Tissue ◽  
Semipermeable Membrane ◽  
G6pd Activity ◽  
Ependymal Cells ◽  
Regional Localization ◽  
Enhanced Resolution ◽  
Membrane Technique ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
The Brain

We examined the histochemical distribution of glucose-6-phosphate dehydrogenase (G6PD) activity in neural tissue using different diffusion barriers. Although polyvinyl alcohol and agar overlays permitted regional localization of G6PD, a semipermeable membrane revealed cellular differences in G6PD activity within populations of neurons. Distribution of G6PD activity in selected regions of the nervous system was examined using the membrane technique. White matter usually exhibited strong G6PD activity. The neuronal somata of the dorsal root ganglia (L4-L6) and anterior horns of the spinal lumbar enlargement demonstrated a variation in activity which was independent of somal size. Satellite cells showed intense activity when the membrane technique was used. Hippocampal pyramidal and granular cells of the dentate gyrus exhibited moderate, uniform G6PD activity, but only weak activity was seen in hippocampal and dentate molecular layers. High levels of activity were observed in the vascular endothelial cells of the brain, spinal cord, and choroid plexus, and in the ependymal cells of the spinal central canal and ventricles of the brain. The superior vestibular nucleus appeared to have little G6PD activity in either the neuron cell bodies or the surrounding parenchyma. The use of a semipermeable membrane for localization of G6PD activity in neural tissues permits enhanced resolution of neuron elements and may provide a more accurate assessment of G6PD activity in histological preparations.

HISTOCHEMICAL DEMONSTRATION OF 20β-HYDROXYSTEROID DEHYDROGENASE

1965 ◽  
Vol 32 (3) ◽  
pp. 337-339 ◽  
Author(s):  
A. H. BAILLIE ◽  
K. C. CALMAN ◽  
M. M. FERGUSON ◽  
D. McK. HART
Keyword(s):  
Dehydrogenase Activity ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Histochemical Demonstration ◽  
Mouse Testis ◽  
Zona Fasciculata ◽  
Mouse Ovary ◽  
Theca Interna ◽  
Human And Mouse

SUMMARY NAD-dependent 20β-hydroxysteroid dehydrogenase activity can be demonstrated histochemically using Nitro-BT. 20β-Hydroxysteroid dehydrogenase activity was found in the Leydig cells of human and mouse testis, in the zona fasciculata of the mouse adrenal and in the theca interna of the mouse ovary.

scholarly journals ON THE IDENTITY OF "NOTHING DEHYDROGENASE"

1965 ◽  
Vol 13 (6) ◽  
pp. 431-433 ◽  
Author(s):  
CHARLES R. SHAW ◽  
ANN L. KOEN
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Gel Electrophoresis ◽  
Histochemical Demonstration ◽  
Starch Gel Electrophoresis ◽  
Quantitative Measurements ◽  
Pure Alcohol ◽  
Mouse Tissues ◽  
Starch Gel

The histochemical demonstration of dehydrogenase activity using tetrazolium sometimes occurs in the absence of added substrate. This occurrence has been called "nothing dehydrogenase". The present study, based on starch gel electrophoresis of mouse tissues and of pure alcohol dehydrogenase, as well as quantitative measurements of the latter, indicates that alcohol dehydrogenase is the major agent of "nothing dehydrogenase" activity.

scholarly journals A METHOD FOR THE HISTOCHEMICAL DEMONSTRATION OF TRIPHOSPHOPYRIDINE NUCLEOTIDE-LINKED l-HEXONATE DEHYDROGENASE ACTIVITY IN KIDNEYS OF SEVERAL SPECIES

1965 ◽  
Vol 13 (7) ◽  
pp. 533-540 ◽  
Author(s):  
KÁROLY BALOGH
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Renal Cortex ◽  
Histochemical Demonstration ◽  
Tetrazolium Salt ◽  
Histochemical Reaction ◽  
Mammalian Tissues ◽  
Histochemical Evidence ◽  
Enzyme Distribution

The described technique utilizes a tetrazolium salt to localize TPN2-linked l-hexonate dehydrogenase activity in unfixed frozen sections of mammalian kidneys. Enzyme diffusion was kept at a minimum by adding PVP to the incubation medium in a final concentration of 20%. Nevertheless, the sites of enzyme activity could not be localized precisely at the cell level. Histochemical evidence for l-hexonate specificity of the enzyme has been presented. A survey of various mammalian tissues revealed strong enzyme activity in the renal cortex of hamsters, rabbits, dogs and humans. In guinea pigs, the histochemical reaction was considerably weaker in heterozygous albino males than in pigmented ones. No enzyme activity was demonstrable in any of the female guinea pigs. Otherwise, the intensity of the histochemical reaction was consistent for a particular species. Although striking species differences were seen in the enzyme distribution pattern, activity was always limited to the epithelial cells of the renal cortex. Other tissues, known to contain the enzyme, failed to reveal an unequivocal histochemical reaction.

Sign in / Sign up

Export Citation Format

Share Document