A quantitative study of N-acetyl-?-glucosaminidase activity in unfixed tissue sections of the guinea-pig thyroid gland

1980 ◽  
Vol 12 (1) ◽  
pp. 87-96 ◽  
Author(s):  
W. R. Robertson
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Quantitative Study ◽  
Tissue Sections

scholarly journals DEMONSTRATION OF PEROXIDASE ACTIVITY IN TISSUE SECTIONS

1964 ◽  
Vol 12 (7) ◽  
pp. 538-544 ◽  
Author(s):  
MAX WACHSTEIN ◽  
ELIZABETH MEISEL
Keyword(s):  
Salivary Gland ◽  
Mast Cells ◽  
Guinea Pig ◽  
Peroxidase Activity ◽  
Striated Muscle ◽  
Acinar Cells ◽  
Hair Follicles ◽  
Tissue Sections ◽  
Pig Skin ◽  
Mammalian Tissues

By using an improved benzidine technique, peroxidase activity can be demonstrated in various locations in mammalian tissues. A relatively formalin resistant enzyme is found in hemoglobin and is also associated with mitochondria of striated muscle and heart. A somewhat less formalin resistant peroxidase occurs in the granules of myeloid and mast cells. A relatively formalin sensitive peroxidase is present in a number of additional locations, e.g. the acinar cells in thyroid and salivary gland, the medulla of the kidney, in hair follicles of the guinea pig skin and Kupffer cells of the liver.

WATER AND ELECTROLYTE DISTRIBUTION IN RAT AND GUINEA-PIG THYROID GLANDS

1971 ◽  
Vol 50 (4) ◽  
pp. 561-576 ◽  
Author(s):  
S. Y. CHOW ◽  
D. M. WOODBURY
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Interstitial Fluid ◽  
Equilibrium Potential ◽  
Excellent Correlation ◽  
Nernst Equation ◽  
Thyroid Glands ◽  
Uptake Studies ◽  
Rat Thyroid ◽  
Cellular Compartments

SUMMARY Procedures for the calculation of the volume of water and the Na+, K+ and Cl− concentrations in the three anatomical compartments of the thyroid (interstitium, follicular cells and lumen) are described. There was an excellent correlation between the volume of water of these three compartments calculated from the total water, [14C]inulin space and histometric measurements of the thyroid gland and the results obtained from 3H2O-uptake studies. Na+ and K+ concentrations in the luminal fluid of rat and guinea-pig thyroid glands were the same as those in the interstitial fluid which is an ultrafiltrate of plasma. Cl− concentration in the luminal fluid of thyroid gland was usually lower than that in the interstitial fluid. The concentration depends on the amount of colloid present in the follicular lumen. In the cellular fluid of the thyroid follicle, Na+ and Cl− concentrations were low and K+ concentration was high. The Cl− equilibrium potential between the interstitial and the cellular compartments, calculated from the corresponding Cl− concentrations by the Nernst equation, was the same as for previous values reported for the intracellular potentials determined by micro-electrode techniques in both rat and guinea-pig thyroid glands. In the rat thyroid, the calculated Cl− equilibrium potential between the interstitial and the luminal compartments was the same as the measured intra-luminal potential. However, in the guinea-pig thyroid the calculated Cl− equilibrium potential between the interstitial and the luminal fluids was considerably higher than the measured intra-luminal potential.

The mitotic index of the thyroid gland in guinea pig and rat

1934 ◽  
Vol 59 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Hilda Friedman ◽  
Leo Loeb
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Mitotic Index

Changes in the Thyroid Gland of Certain Mammals and Birds Following Hypophysectomy

1935 ◽  
Vol 12 (4) ◽  
pp. 337-347
Author(s):  
I. W. ROWLANDS
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Similar Condition ◽  
Histological Difference

1. The weight of the thyroid of the normal anoestrous ferret of both sexes is approximately half that of the oestrous ferret, but there is little histological difference. 2. Hypophysectomy during oestrus or anoestrus decreases the weight of the thyroid of the ferret to a level slightly lower than that for the normal anoestrous ferret. 3. In the hibernating hedgehog the thyroid becomes hypotrophic (Adler, 1920), and a similar condition is found in the hypophysectomised animal. 4. No change in weight of the thyroid of the guinea-pig is noticed following hypophysectomy, but atrophy of the vesicular epithelium and distention of the vesicles with non-vacuolated colloid is regularly observed. Hypophysectomy in birds leads to similar atrophic changes in the thyroid.

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