Formation of functional synaptic connections between heterogeneous brain formations in organotypic nerve tissue culture

1981 ◽  
Vol 12 (5) ◽  
pp. 311-317 ◽  
Author(s):  
I. V. Viktorov ◽  
I. N. Sharonova
1998 ◽  
Vol 125 (3) ◽  
pp. 294-297
Author(s):  
V. Kh. Khavinson ◽  
N. I. Chalisova ◽  
V. B. Okulov

1975 ◽  
Vol 80 (4) ◽  
pp. 1234-1236
Author(s):  
V. B. Gervazieva ◽  
I. G. Mikhailov ◽  
T. D. Kanareitseva

1972 ◽  
Vol 21 (3) ◽  
pp. 204-212 ◽  
Author(s):  
J. F. Schneider ◽  
R. I. Carp ◽  
E. H. Belisle ◽  
C. E. Rue

1968 ◽  
Vol 43 (3) ◽  
pp. 161-165 ◽  
Author(s):  
Edmund B. Masurovsky ◽  
Richard P. Bunge

1970 ◽  
Vol 8 (1) ◽  
pp. 3-6 ◽  
Author(s):  
M Matiur Rahman ◽  
M Salimuzzaman ◽  
MD Billal Alam ◽  
MA Rouf ◽  
M Jakir Hossain ◽  
...  

This descriptive study in the Infectious Disease Hospital, Dhaka, on rabies was carried out to see the patterns of presentation, age group at risk, state of vaccination following animal exposure and the interval between animal exposure and the development of the disease. Of 684 cases of rabies from June 1999 to November 2003, majority (64.18%) of the victims were within 15 years of age. Male gender (76.75%) and rural people (86.40%) constituted the bulk of the cases. 80.26% of cases had WHO category III exposure. Most of the cases were not vaccinated (86.04%). Among 93 cases who received vaccine, only 9.67% took tissue culture vaccine and 90.3% were treated with nerve tissue vaccine. None of the 684 cases were treated with RIG. Dog was the principal animal responsible for 95.76% cases. 65.93% of the cases developed the disease between 31 to 100 days after the animal exposure. DOI = 10.3329/jom.v8i1.1370 J MEDICINE 2007; 8 : 3-6   


Author(s):  
Adrian F. van Dellen

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.


Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.


Author(s):  
Amankwah K.S. ◽  
A.D. Weberg ◽  
R.C. Kaufmann

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.


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