Crystal structures of bile salts: Sodium taurocholate

1991 ◽  
Vol 11 (3) ◽  
pp. 247-256 ◽  
Author(s):  
Anna Rita Campanelli ◽  
Sofia Candeloro De Sanctis ◽  
Angelo Antonio D'Archivio ◽  
Edoardo Giglio ◽  
Lucid Scaramuzza
Keyword(s):  
Crystal Structures ◽  
Bile Salts ◽  
Sodium Taurocholate

Effect of intestinal resection on bile salt absorption in dogs

1965 ◽  
Vol 208 (2) ◽  
pp. 363-369 ◽  
Author(s):  
M. R. Playoust ◽  
Leon Lack ◽  
I. M. Weiner
Keyword(s):  
Bile Salt ◽  
Bile Salts ◽  
Enterohepatic Circulation ◽  
Sodium Taurocholate ◽  
Intestinal Resection ◽  
High Fat ◽  
Salt Absorption ◽  
Complete Failure ◽  
Fat Meal

The efficiency of intestinal absorption of bile salts was evaluated by studying the rate of disappearance of radioactivity from the bile of dogs after the intravenous administration of sodium taurocholate-24-C14. Bile was sampled through an indwelling tube in the gall bladder. One day after a high-fat meal normal dogs retained 48% of the radioactivity; dogs with resection of the jejunum retained 48%, whereas those with resection of the ileum retained only 3% in the bile. This is consistent with previous observations that the ileum is the site of bile salt absorption in vitro and in anesthetized animals. Animals with resection of the ileum exhibited significant steatorrhea; however, three-fourths of the ingested fat was absorbed in spite of almost complete failure to absorb bile salts. This indicates that fat and bile salts are not normally absorbed together. Elimination of enterohepatic circulation of bile salts by resection of the ileum contributes to the observed steatorrhea.

Effect of glycodihydrofuisidate on sulfobromophthalein transport maximum in the hamster

1976 ◽  
Vol 231 (6) ◽  
pp. 1875-1878 ◽  
Author(s):  
Y Delage ◽  
M Dumont ◽  
S Erlinger
Keyword(s):  
Bile Salt ◽  
Transport System ◽  
Bile Salts ◽  
Sodium Taurocholate ◽  
Specific Effect ◽  
Biliary Secretion ◽  
Biliary Lipid ◽  
Lipid Secretion ◽  
Dye Transport ◽  
Cholesterol Secretion

The effect on sulfobromophathalein transport maximum (Tm) and biliary lipid secretion of sodium glyco-24,25-dihydrofusicate, a micelle-forming compound secreted into bile, has been studied in the hamster and compared to that of a physiological bile salt, sodium taurocholate. Biliary phospholipid and cholesterol secretion increased both during glycodihydrofusidate and taurocholate administration, an observation which suggest that both compounds increased th biliary secretion of micelle-forming compounds. In contrast, only taurocholate increased sulfobromophthalein Tm into bile, while glycodihydrofusidate administration decreased it. This observation suggests that the increase in sulfobromophthalein Tm observed during taurocholate administration is not the result of micellar sequestration. It could rather be the consequence of a specific effect of bile salts on the dye transport system.

Biliary Inter-Relationship between Phospholipid, Bilirubin and Taurocholate in the Anaesthetized Rat

1984 ◽  
Vol 67 (5) ◽  
pp. 499-504 ◽  
Author(s):  
J. J. García-Marín ◽  
A. Esteller
Keyword(s):  
Body Weight ◽  
Bile Flow ◽  
Bile Salts ◽  
Mixed Micelle ◽  
Enterohepatic Circulation ◽  
Important Determinant ◽  
Sodium Taurocholate ◽  
Micelle Formation ◽  
Mixed Micelle Formation ◽  
Anaesthetized Rat

1. The interference between biliary phospholipid and bilirubin secretion was investigated in rats with bile fistulae, under conditions of normal and maximal bilirubin secretion. The enterohepatic circulation of bile salts was interrupted and the animals received infusions of sodium taurocholate, a micelle-forming physiological bile salt. 2. Sodium taurocholate infusion (0.19 μmol min−1 100 g−1 body weight) induced an increase in bile flow and phospholipid secretion, while basal bilirubin secretion was not increased. 3. Bilirubin infusion (0.26 μmol min−1 100 g−1 body weight) induced a decrease in basal and taurocholate-stimulated phospholipid secretion. Biliary mixed micelle formation was presumably altered during bilirubin infusion, although bile taurocholate concentration, taurocholate secretion rate and bile flow were not modified. 4. When sodium taurocholate was infused during bilirubin-decreased phospholipid secretion, this secretion was restored but maximal biliary bilirubin secretion was not increased. 5. These results provide circumstantial evidence for the hypothesis that mixed micelle formation is not an important determinant of maximal bilirubin transport into bile.

How does bile salt penetration affect the self-assembled architecture of pluronic P123 micelles? – light scattering and spectroscopic investigations

2015 ◽  
Vol 17 (30) ◽  
pp. 19977-19990 ◽  
Author(s):  
Arpita Roy ◽  
Niloy Kundu ◽  
Debasis Banik ◽  
Jagannath Kuchlyan ◽  
Nilmoni Sarkar
Keyword(s):  
Light Scattering ◽  
Bile Salt ◽  
Bile Salts ◽  
Triblock Copolymer ◽  
Sodium Taurocholate ◽  
Sodium Deoxycholate ◽  
The Self ◽  
Spectroscopic Investigations ◽  
Pluronic P123 ◽  
Supramolecular Aggregate

The triblock copolymer of the type (PEO)20–(PPO)70–(PEO)20 (P123) forms a mixed supramolecular aggregate with different bile salts, sodium deoxycholate (NaDC) and sodium taurocholate (NaTC), having different hydrophobicity.

scholarly journals Extended Abstract: Deficiency of Sodium Taurocholate Cotransporting Polypeptide (SLC10A1): A New Inborn Error of Metabolism with an Attenuated Phenotype

2017 ◽  
Vol 35 (3) ◽  
pp. 259-260 ◽  
Author(s):  
Frédéric M. Vaz ◽  
Hidde H. Huidekoper ◽  
Coen C. Paulusma
Keyword(s):  
Bile Salts ◽  
Clinical Presentation ◽  
Enterohepatic Circulation ◽  
Sodium Taurocholate ◽  
Elevated Plasma ◽  
Mild Phenotype ◽  
Functional Studies ◽  
Conjugated Bile Salts

We present the first patient with a defect in the Na+-taurocholate cotransporting polypeptide SLC10A1 (NTCP), which plays a key role in the enterohepatic circulation of bile salts. The clinical presentation of the child was mild and the child showed no signs of liver dysfunction or pruritus despite extremely elevated plasma bile salt levels (>100-fold upper-limit of normal). A homozygous point mutation was found in the SLC10A1 gene (resulting in amino acid change R252H) and functional studies confirmed the pathogenicity of the mutation. This confirms the role of NTCP as the major transporter of conjugated bile salts into the liver as part of the enterohepatic circulation and shows that other transporters partly can take over its function, resulting in a relatively mild phenotype. This work was published previously in [Vaz et al.: Hepatology 2015;61:260-267] and supplemented with some follow-up information of the patient.

scholarly journals DECREASE OF PERMEABILITY AND ANTAGONISTIC EFFECTS CAUSED BY BILE SALTS

1919 ◽  
Vol 1 (4) ◽  
pp. 405-408 ◽  
Author(s):  
W. J. V. Osterhout
Keyword(s):  
Bile Salts ◽  
Sodium Taurocholate ◽  
Antagonistic Effects ◽  
Pure Substances

Sodium taurocholate is able to produce a decrease in permeability and to antagonize NaCl. This confirms the hypothesis that antagonistic relations can be predicted from studies on the permeability of pure substances.

Facilitation of hepatic uptake of phenol 3,6-dibromphthalein disulfonate by taurocholate.

1977 ◽  
Vol 232 (6) ◽  
pp. E560
Author(s):  
Y Marinovic ◽  
J C Glasinovic ◽  
B Semelle ◽  
J F Boivieux ◽  
S Erlinger
Keyword(s):  
Bile Acid ◽  
Initial Velocity ◽  
Bile Salts ◽  
Sodium Taurocholate ◽  
Hepatic Uptake ◽  
Disappearance Rate ◽  
Plasma Disappearance Rate ◽  
Common Site ◽  
Half Saturation Constant ◽  
Uptake Process

The influence of sodium taurocholate (TC) on the hepatic uptake of phenol 3,6-dibromphthalein disulfonate (dibromosulphophthalein, DBSP) was examined in the dog. Single injections of DBSP (7.5-83.3 mg-kg-1) were given intravenously and the initial velocity of uptake (V) of the dye was calculated from the plasma disappearance rate measured during the 1st 5 min. It was observed that: a) the initial velocity of uptake of DBSP increased with the dose (D) in a nonlinear way, a finding consistent with Michaelis-Menten kinetics; the maximal initial velocity of uptake (Vmax) was 7.5+/-1.0 (SD) mg-min-1-kg body wt-1, and the half-saturation constant (Kd) was 27.7+/-7.0 (SD) mg-kg-1; b) when a TC infusion was given prior to the DBSP injection, the initial disappearance of the dye was more rapid than in the absence of the bile acid; Vmax increased to 12.3+/-2.0 (SD) mg-min-1-kg-1 (P less than 0.001) and Kd increased to 50.3+/-12.8 (SD) mg-kg-1 (P less than 0.001). These results indicate that: 1) the uptake of DBSP by the liver cell is a saturable process; 2) TC increases both the Vmax, suggesting a facilitation of the uptake process, and the Kd, suggesting competition for a common site. This effect of TC on DBSP uptake is similar to the previously described effect of this bile salt on BSP excretion into bile and suggests an interaction of bile salts with the uptake process of dyes.

scholarly journals THE RELATION OF PROTEIN, LIPOIDS AND SALTS TO THE WASSERMANN REACTION

1909 ◽  
Vol 11 (1) ◽  
pp. 84-99 ◽  
Author(s):  
Hideyo Noguchi
Keyword(s):  
Bile Salts ◽  
Sodium Taurocholate ◽  
Sodium Cholate ◽  
Secondary Syphilis ◽  
Quantitative Relation ◽  
The Sun ◽  
Peptic Digestion ◽  
Rapid Destruction ◽  
Wassermann Reaction ◽  
Active Substances

1. The high value in respect to complement-binding exhibited by blood sera from syphilitics and spinal fluids from general paralytics is associated with an excessively high content of globulin, but there does not exist a direct quantitative relation between the two. Cases of secondary syphilis which have been under prolonged and proper medication do not exhibit the globulin increase and usually fail to give the Wassermann reaction. The active substances entering into the Wassermann reaction are precipitable with the globulin and chiefly with the euglobulin fraction of the fluids. 2. Temperatures of 70° to 76° C. destroy the active substances. Exposed to sunlight the active substances deteriorate slowly. A photodynamic substance such as eosin, under the direct influence of the sun, brings about their complete and rapid destruction. This effect does not occur in the dark. The active substances are subject to tryptic and peptic digestion and are destroyed by weak acids and alkalies. 3. The active substances in the blood sera and spinal fluids cannot be separated from them or from the globulin precipitate by alcohol. 4. There are contained in the alcoholic extracts of normal and syphilitic blood and organs certain acetone-soluble lipoids which possess high antigenic values for the Wasserman reaction. Cholesterin is inactive and the bile salts less active than the lipoidal bodies. 5. Sodium cholate is about as active as sodium taurocholate and glycocholate, but neurin and cholin are inactive.

Rapid Spectrofluorometric Assay for Total Bile Salts in Bile

1972 ◽  
Vol 18 (9) ◽  
pp. 987-991 ◽  
Author(s):  
Edward J Mroszczak ◽  
Sidney Riegelman
Keyword(s):  
Fluorescence Intensity ◽  
Bile Salts ◽  
Sodium Taurocholate ◽  
Alternative Methods ◽  
Excitation Wavelength ◽  
Extraction Step ◽  
Fluorescent Products ◽  
Concentrated Sulfuric Acid ◽  
Liquid Chromatographic ◽  
Average Fluorescence

Abstract An assay is presented for total bile salts in bile. The procedure involves one precipitation and extraction step and then treatment with concentrated sulfuric acid to produce fluorescent products, which are then measured at an excitation wavelength of 470 nm and emission wavelength of 505 nm. With sodium taurocholate as the standard (fluorescence intensity of 1.00), the average fluorescence intensity of equimolar concentrations of the taurine and glycine conjugates of cholic, deoxycholic, and chenodeoxycholic acid is 1.01 (standard dev., 0.05). The average recovery of the six bile salts from pooled rat bile is 95.7% (standard dev., 8.5). The assay compares well with the established gas—liquid chromatographic and enzymatic methods, but it is simpler, faster, less costly, and just as reliable as the alternative methods.

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