Elevation of an?(1,3) fucosyltransferase activity correlated with apoptosis in the human colon adenocarcinoma cell line, HT-29

1996 ◽  
Vol 13 (6) ◽  
pp. 1021-1029 ◽  
Author(s):  
Suguru Akamatsu ◽  
Shin Yazawa ◽  
Koichi Zenita ◽  
Hisanori Matsumoto ◽  
Tetsuya Tachikawa ◽  
...  
Keyword(s):  
Cell Line ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma

Role of hesperetin (a natural flavonoid) and its analogue on apoptosis in HT-29 human colon adenocarcinoma cell line – A comparative study

2012 ◽  
Vol 50 (3-4) ◽  
pp. 660-671 ◽  
Author(s):  
Gunasekaran Sivagami ◽  
Rajamanickam Vinothkumar ◽  
Christo Paul Preethy ◽  
Anvarbatcha Riyasdeen ◽  
Mohammad Abdulkader Akbarsha ◽  
...  
Keyword(s):  
Comparative Study ◽  
Cell Line ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma

TNF-α modulates the differentiation induced by butyrate in the HT-29 human colon adenocarcinoma cell line

2000 ◽  
Vol 36 (14) ◽  
pp. 1844-1852 ◽  
Author(s):  
M Kovařı́ková ◽  
J Pachernı́k ◽  
J Hofmanová ◽  
Z Zadák ◽  
A Kozubı́k
Keyword(s):  
Cell Line ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Tnf Α ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma

scholarly journals Molecular identification and structural requirement of vasoactive intestinal peptide (VIP) receptors in the human colon adenocarcinoma cell line, HT-29

1985 ◽  
Vol 231 (1) ◽  
pp. 139-143 ◽  
Author(s):  
A Couvineau ◽  
M Rousset ◽  
M Laburthe
Keyword(s):  
Cell Line ◽  
Vasoactive Intestinal Peptide ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cell Line ◽  
Structural Requirement ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma ◽  
Vip Receptor

The human colon adenocarcinoma cell line HT-29 in culture exhibits a cyclic AMP production system highly sensitive to vasoactive intestinal peptide (VIP), making HT-29 cells a unique cultured cell system for studying the mechanism of VIP action [Laburthe, Rousset, Boissard, Chevalier, Zweibaum & Rosselin (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 2772-2775]. The quantitative characteristics of VIP receptors in HT-29 cells and their structural requirement and molecular size were studied. 125I-labeled VIP bound in a time-dependent manner to HT-29 cell homogenates. At equilibrium (60 min incubation at 30 degrees C), unlabelled VIP in the 0.01-10 nM concentration range competed with 125I-VIP for binding to cell homogenates. Scatchard analysis of binding data gave a straight line, indicating that VIP bound to a single population of sites with a KD of 0.12 +/- 0.02 nM and a capacity of 120 +/- 9 fmol/mg of protein. The structural requirement of these receptors was studied with peptides structurally related to VIP, either natural or synthetic. Several peptides inhibited 125I-VIP binding to HT-29 cell homogenates with the following order of potency, which is typical of the human VIP receptor: VIP (IC50 = 0.1 nM) greater than VIP-(2-28)-peptide (IC50 = 13 nM) greater than human growth hormone releasing factor (IC50 = 56 nM) greater than peptide histidine isoleucine amide (IC50 = 80 nM) greater than secretin (IC50 greater than 10 000 nM). To characterize the molecular component(s) of the VIP receptor in HT-29 cells, 125I-VIP was covalently bound to cell homogenates by using the cross-linker dithiobis(succinimidyl propionate). Sodium dodecyl sulphate/polyacrylamide-gel autoradiographic studies of affinity-labelled cell homogenates revealed two major bands, corresponding to 125I-VIP-protein complexes of Mr 66 000 and 16 000. The labelling of the Mr-66 000 component was specific, since it was abolished by native VIP, whereas that of the Mr-16 000 component was not. Densitometric scanning of autoradiographs indicated that the labelling of the Mr-66 000 complex was inhibited by low VIP concentrations in the 0.1-10 nM range (IC50 = 0.6 nM), but was unaffected by 1 microM-glucagon or octapeptide of cholecystokinin. It was also decreased by VIP-(2-28)-peptide with a potency 1% that of VIP. Assuming that one molecule of 125I-VIP bound per molecule of protein, one protein of Mr 63 000 was identified as a component of the VIP receptor in HT-29 cells.

Cytotoxic alkaloids against human colon adenocarcinoma cell line (HT-29) from the seed embryos of Nelumbo nucifera

2017 ◽  
Vol 27 (3) ◽  
pp. 939-943 ◽  
Author(s):  
Waraluck Chaichompoo ◽  
Ratchanaporn Chokchaisiri ◽  
Nuttapon Apiratikul ◽  
Arthit Chairoungdua ◽  
Boon-ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Cell Line ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Nelumbo Nucifera ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Human Colon Adenocarcinoma
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