Automated enzymatic determination of ATP in bovine muscle tissue

1981 ◽  
Vol 173 (5) ◽  
pp. 362-364 ◽  
Author(s):  
Nel Haagsma ◽  
Betty G. M. Gortemaker
Keyword(s):  
Muscle Tissue ◽  
Bovine Muscle ◽  
Enzymatic Determination

Automated enzymatic determination of glycogen in bovine muscle tissue

1981 ◽  
Vol 172 (6) ◽  
pp. 454-456 ◽  
Author(s):  
Nel Haagsma ◽  
Klaas J. W. Kruithof ◽  
Betty G. M. Gortemaker
Keyword(s):  
Muscle Tissue ◽  
Bovine Muscle ◽  
Enzymatic Determination

scholarly journals Determination of Zeranol and its Metabolites in Bovine Muscle Tissue with Gas Chromatography-Mass Spectrometry

2012 ◽  
Vol 56 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Iwona Matraszek-Żuchowska ◽  
Barbara Woźniak ◽  
Jan Żmudzki
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Muscle Tissue ◽  
Solid Phase ◽  
Performance Criteria ◽  
Gas Chromatography Mass Spectrometry ◽  
The European Union ◽  
Bovine Muscle ◽  
Chromatography Mass Spectrometry

Abstract This paper describes the quantitative method of determination of chosen substances from resorcylic acid lactones group: zeranol, taleranol, α-zearalenol, β-zearalenol, and zearalanone in bovine muscle tissue. The presented method is based on double diethyl ether liquid-liquid extraction (LLE), solid phase extraction (SPE) clean up, and gas chromatography mass spectrometry (GC-MS) analysis. The residues were derivatised with a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide, ammonium iodide, and DL-dithiothreitol (1,000:2:5, v/w/w). The GC-MS apparatus was operated in positive electron ionisation mode. The method was validated according to the European Union performance criteria pointed in Decision Commission 2002/657/EC. The average recoveries of all analytes at 1 μg kg-1 level were located between 83.7% and 94.5% values with the coefficients of variation values <25%. The decision limits (CCα) and detection capabilities (CCβ) for all analytes ranged from 0.58 to 0.82 μg kg-1 and from 0.64 to 0.94 μg kg-1, respectively. The procedure has been accredited and is used as a screening and confirmatory method in control of hormone residues in animal tissues.

scholarly journals Multiresidue Liquid Chromatographic Method for Determining Residues of Mono- and Dibasic Penicillins in Bovine Muscle Tissues

1998 ◽  
Vol 81 (6) ◽  
pp. 1113-1120 ◽  
Author(s):  
Joe O Boison ◽  
Lily J -Y Keng
Keyword(s):  
Muscle Tissue ◽  
Phosphate Buffer ◽  
Chromatographic Method ◽  
Sodium Thiosulfate ◽  
Laboratory Method ◽  
Penicillin G ◽  
Bovine Muscle ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method with UV detection at 325 nm was developed for simultaneous determination of amoxicillin, ampicillin, penicillin G, and cloxacillin residues in bovine muscle tissue as their mercaptide derivatives. The penicillins are extracted from bovine tissues with 0.1 M phosphate buffer (pH 8.5), cleaned up on a t-C18 Sep-Pak cartridge, and eluted with 2 ml acetonitrile. After the acetonitrile in the eluate is evaporated to dryness, the residue is dissolved in 200 |µL (40 + 60, v/v) aceton itrile-phosphate buffer (pH 6.5) and derivatized with acetic anhydride and mercuric chloride in the presence of 1,2,4-triazole at 65°C for 30 min. Gradient analysis on a Spherisorb 5 µm ODS(2) (octadecyl silane) analytical column using a binary mobile phase consisting of acetonitrile and 0.10M phosphate buffer (pH 6.5) in the presence of 0.0157M sodium thiosulfate at 1 mL/min permits determination of each intact penicillin in bovine muscle tissue at ≥10 ppb with recoveries ≥72%. This laboratory method provides detection sensitivities equivalent to those of rapid tests used for screening β-lactam drug residues in bovine tissue samples for regulatory enforcement.

THE EFFECT OF METHOD OF SAMPLE PREPARATION UPON THE DETERMINATION OF SOLUBILIZED INTRAMUSCULAR COLLAGEN FROM BOVINE MUSCLE BY QUANTITATION OF ITS HYDROXYPROLINE CONTENT

1980 ◽  
Vol 60 (3) ◽  
pp. 627-634 ◽  
Author(s):  
L. E. JEREMIAH ◽  
A. C. MURRAY ◽  
A. H. MARTIN
Keyword(s):  
Sample Preparation ◽  
Liquid Nitrogen ◽  
Muscle Tissue ◽  
Recovery Rate ◽  
Freeze Drying ◽  
Soluble Fraction ◽  
Collagen Content ◽  
Hydroxyproline Content ◽  
Bovine Muscle

A total of 91 muscle samples were utilized in a series of three separate but related experiments to evaluate possible effects of method of sample preparation upon the yields of intramuscular hydroxyproline from the heat-soluble, insoluble and combined fractions of bovine muscle tissue. The combined results of these experiments indicate that yields of intramuscular hydroxyproline, and thereby yields of intramuscular collagen, obtained in different studies can not be compared unless the same muscle is evaluated and the same method of sample preparation is employed in both and/or all studies in which yields are to be compared. Results from these experiments may also suggest that sample preparation, in which samples are frozen in liquid nitrogen, results in underestimation of intramuscular hydroxyproline content, and thereby intramuscular collagen content, from the heat-soluble fraction as evidenced by: (1) the substantially lower yields of intramuscular hydroxyproline from the heat-soluble fraction of samples frozen in liquid nitrogen; (2) the fact that added hydroxyproline was completely recovered in the heat-soluble fraction of muscle during preliminary experiments; and (3) the fact that method of sample preparation (freeze drying vs. direct homogenization) did not influence the recovery rate or result in overestimation of added hydroxyproline.

Enzymatic Determination of Hydroxysteroids in Human Skin Surface Lipids1

1963 ◽  
Vol 41 (5) ◽  
pp. 265-268 ◽  
Author(s):  
Thomas J Cook ◽  
Allan L Lorincz ◽  
Alan R Spector
Keyword(s):  
Human Skin ◽  
Skin Surface ◽  
Enzymatic Determination

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

Sign in / Sign up

Export Citation Format

Share Document