Plasma protein recovery from spent tissue culture fluid

1991 ◽  
Vol 13 (4) ◽  
pp. 261-264 ◽  
Author(s):  
P. K. Ng ◽  
C. Figueroa ◽  
G. Mitra
Keyword(s):  
Tissue Culture ◽  
Plasma Protein ◽  
Culture Fluid ◽  
Protein Recovery

scholarly journals BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

1960 ◽  
Vol 112 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Akira Tokuda ◽  
Hideo Hayashi ◽  
Kinishiro Matsuba
Keyword(s):  
Tissue Culture ◽  
Protease Activity ◽  
Biochemical Study ◽  
Culture Fluid ◽  
Partial Purification ◽  
Trichloracetic Acid ◽  
Heat Stable ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Cellular Antigen

Decrease in the protease activity of the culture fluid observed at later stages of the antigen-antibody reaction is believed to be due to the release of an inhibitor by the cells. The inhibitor was submitted to partial purification: it is heat-stable, non-precipitated by trichloracetic acid and non-dialyzable. It inhibits certain cellular and tissue proteases and papain but is inactive against trypsin. It is suggested that the balance between protease and anti-protease released may determine the intensity, extent, and duration of certain sensitization phenomena.

scholarly journals Immunosuppressive activity of a subline of the mouse EL-4 lymphoma. Evidence for minute virus of mice causing the inhibition.

1976 ◽  
Vol 143 (1) ◽  
pp. 187-205 ◽  
Author(s):  
G D Bonnard ◽  
E K Manders ◽  
D A Campbell ◽  
R B Herberman ◽  
M J Collins
Keyword(s):  
Tissue Culture ◽  
Culture Fluid ◽  
Inhibitory Factor ◽  
Minute Virus Of Mice ◽  
G Cells ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Lymphocyte Cultures ◽  
Minute Virus ◽  
Kilham Rat Virus

Filtered culture fluids from the early in vitro passages of a subline of the C57BL/6 mouse EL-4 lymphoma, EL-4(G-), were strongly inhibitory for BABL/c vs. C57BL/6 mixed lymphocyte cultures (MLC). The inhibitory activity could be preserved by storage at -75 degrees C or 4 degrees C, thus allowing its further characterization. The inhibitory factor was particulate (nondialyzable, sedimentable at 100,000 g for 1 h), very small (recovered after 0.10 mum filtration), sensitive to UV irradiation, but heat stable (56 degrees C, 1 h) and resistant to chloroform. It was infectious, since later, noninhibitory passages of EL-4(G-) tissue culture cells became strongly inhibitory upon inoculation with the culture fluid. This data was consistent with the inhibitory factor being an infectious virus. Virus analysis by mouse antibody production tests revealed that viruses were indeed present in EL-4(G-) ascites cells and in the culture fluid, and not in a late passage of EL-4(G-) tissue culture cells which were not inhibitory. Neutralization of the inhibitory factor was achieved by pretreatment with ascitic fluid or with the sera raised against those (EL-4(G-)-derived materials which contained viruses. Mouse reference immune sera against minute virus of mice (MVM) completely neutralized the inhibitory factor in the culture fluid or in EL-4(G-) ascites cells. Two prototype MVM strains, and one Kilham rat virus preparation, did not inhibit the mouse MLC. Thus, the possibility exists that a variant of MVM, or an unidentified virus, has been grown and selected for in EL-4(G-) cells and recognized, due to its immunosuppressive characteristics. In any event, immunosuppression by EL-4(G-) cells was not mediated by the tumor cells, their metabolic products, or associated endogenous type C viruses, but by an exogenous virus, most likely a variant MVM with immunosuppressive characteristics. This adds weight to a parallel observation from our laboratory on the immunosuppressive effects of Kilham rat virus in rat lymphocyte cultures.

Stability of visna virus in infectious tissue culture fluid

1961 ◽  
Vol 10 (4) ◽  
pp. 501-509 ◽  
Author(s):  
Halld�r Thormar
Keyword(s):  
Tissue Culture ◽  
Culture Fluid ◽  
Visna Virus

scholarly journals Enhancement of IgE-mediated histamine release from human basophils by viruses: role of interferon.

1977 ◽  
Vol 145 (4) ◽  
pp. 892-906 ◽  
Author(s):  
S Ida ◽  
J J Hooks ◽  
R P Siraganian ◽  
A L Notkins
Keyword(s):  
Tissue Culture ◽  
Antiviral Activity ◽  
Histamine Release ◽  
Influenza A ◽  
Culture Fluid ◽  
Soluble Factor ◽  
Release Histamine ◽  
Ige Mediated ◽  
Human Basophils

Human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen E or anti-IgE. Leukocyte cultures incubated with virus (i.e. HSV-1, Influenza A, and Adeno-1) but not exposed to ragweed antigen E or anti-IgE fail to release histamine. If, however, leukocyte cultures are first exposed to virus and then to ragweed antigen E or anti-IgE, significant enhancement of histamine release occurs. Both infectious and inactivated virus enhance histamine release and the degree of enhancement is related to the concentration of virus and the length of the incubation. Tissue culture fluid harvested 8 h after exposure of leukocytes to virus contains a soluble factor which is capable of enhancing histamine release when added to fresh leukocyte cultures. This factor has all the properties of interferon including species specificity and cannot be dissociated from the antiviral activity of interferon. Moreover, both known inducers of interferon (poly I:poly C) and standard preparations of interferon are capable of enhancing histamine release. The enhancement of histamine release by interferon represents a new biological role for interferon.

Effect of Zinc on Leucocyte Sodium Transport In Vitro

1978 ◽  
Vol 54 (5) ◽  
pp. 585-587 ◽  
Author(s):  
J. Patrick ◽  
J. Michael ◽  
M. N. Golden ◽  
B. E. Golden ◽  
P. J. Hilton
Keyword(s):  
Tissue Culture ◽  
Sodium Transport ◽  
Zinc Concentration ◽  
Culture Fluid ◽  
Sodium Content ◽  
Cell Water ◽  
Sodium Influx ◽  
Sodium Efflux

1. In a preparation of human leucocytes maintained in tissue culture fluid, increasing the extracellular zinc concentration leads to a significant increase in both ouabain-sensitive sodium efflux and in sodium influx. 2. Cell water and sodium content do not alter significantly with increasing extracellular zinc concentration. 3. A small increase in the ouabain-insensitive sodium efflux can be demonstrated when the external zinc concentration is raised from 0·75 μmol/l to 90 μmol/l.

Leucocyte Sodium Fluxes in Normal Plasma and Tissue Culture Fluid

1985 ◽  
Vol 69 (s12) ◽  
pp. 83P-83P
Author(s):  
L.L. Ng ◽  
R.F. Smith ◽  
T.D.R Hockaday
Keyword(s):  
Tissue Culture ◽  
Culture Fluid ◽  
Normal Plasma

scholarly journals A comparative study of a plaque and quantal method for assaying the neutralizing activity of antisera to type 1 poliovirus

1965 ◽  
Vol 63 (3) ◽  
pp. 311-325 ◽  
Author(s):  
P. G. Higgins
Keyword(s):  
Tissue Culture ◽  
Comparative Study ◽  
Poisson Distribution ◽  
Culture Fluid ◽  
Neutralizing Activity ◽  
The Mean ◽  
Increase In Accuracy ◽  
The Relationship ◽  
Expected Increase

Sera from two rabbits bled after one, two and three inoculations of concentrated type 1 poliovirus tissue culture fluid were assayed for neutralizing activity by a quantal and a plaque method. The plaque counts in these tests have been shown to approximate to a Poisson distribution as determined by the relationship between the variance and the mean of replicate counts. However, there is a considerable scatter of the points in such a comparison and this accounts for the absence of the expected increase in accuracy of this plaque method over the quantal.The relationship between the antibody content of the sera determined by the two methods is non-linear. This can be attributed to inhomogeneity of the antibody produced which increases with the number of antigenic stimuli given.

scholarly journals Cytopathic Effect of Vero Cells Adapted Bangladeshi Strain of Peste Des Petits Ruminants (PPR) Virus in Cell Culture

2021 ◽  
Author(s):  
MSI Siddiqui ◽  
Anja Globig ◽  
Bernd Hoffmann ◽  
MN Islam ◽  
Islam ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Inclusion Bodies ◽  
Cytopathic Effect ◽  
Cell Monolayer ◽  
Culture Fluid ◽  
Vero Cells ◽  
Cover Slip ◽  
Ppr Virus ◽  
Complete Detachment ◽  
Bangladeshi Strain

Abstract The present study described the cytopathic effect of PPR virus presently being used in serial passages at level of 60th in Vero cells and infected tissue culture fluid was used in this study as viral inoculum. Vero cells were grown on cover slip & were infected with tissue culture fluid at a fixed multiplicity of infection (MOI) 0.01. The infected cover slip along with control were stained with H&E stain at periodic intervals and cytopathic effect was studied with microscope. The cytopathic effect (CPE) was visible at first from 24 hpi and the Vero cells showed initial cell rounding, aggregation and syncytial development. Development of inclusion bodies and cell degradation was noticed by 72 hpi. Complete detachment of the cell monolayer was observed by 84 hpi. It is concluded that, development of numerous inclusion bodies is the indication of well adaptation & extensive multiplication of PPRV in Vero cells.

Zinc Transport in Normal Human Leucocytes: Dependence upon Media Composition

1980 ◽  
Vol 59 (5) ◽  
pp. 353-357 ◽  
Author(s):  
R. B. Jones ◽  
P. J. Hilton ◽  
J. Michael ◽  
J. Patrick ◽  
V. E. Johnson
Keyword(s):  
Tissue Culture ◽  
Rate Constant ◽  
Culture Medium ◽  
Culture Fluid ◽  
Efflux Rate ◽  
Rapid Phase ◽  
Efflux Rate Constant ◽  
Zinc Concentrations ◽  
Normal Human ◽  
Krebs Buffer

1. The transport of zinc has been studied in normal human leucocytes incubated in both a tissue culture medium and Krebs buffer. 2. 65Zn influx is characterized by an initial rapid phase followed by a slower influx. 65Zn influx is directly dependent upon the extracellular zinc concentration. 3. Net zinc influx could only be demonstrated in Krebs buffer at zinc concentrations in excess of 4.3 μmol/l and in tissue culture fluid at zinc concentrations in excess of 43.1 μmol/l. 4. A 65Zn efflux rate constant of approximately 1.0 per h was observed in both 4.3 and 15.4 μmol of zinc/l of Krebs buffer or tissue culture fluid. 5. In a zero external zinc Krebs buffer the 65Zn efflux rate constant fell to 0.57 per h and was accompanied by a small net zinc efflux.

scholarly journals STUDIES OF MOUSE POLYOMA VIRUS INFECTION

1959 ◽  
Vol 109 (5) ◽  
pp. 439-447 ◽  
Author(s):  
Isadore Brodsky ◽  
Wallace P. Rowe ◽  
Janet W. Hartley ◽  
William T. Lane
Keyword(s):  
Tissue Culture ◽  
Ethyl Alcohol ◽  
Ultraviolet Irradiation ◽  
Horse Serum ◽  
Virus Titer ◽  
Basal Medium ◽  
Culture Fluid ◽  
Polyoma Virus ◽  
The Stability ◽  
Infectivity Titer

Mouse polyoma virus was stored at 4° and –60°C. for 8 weeks without any loss in hemagglutination or infectivity titer; storage at 37° for 8 weeks reduced infectivity titer by approximately 2.5 log10 units. Repeated freezing and thawing of infectious tissue culture fluid had no effect on virus titer. The stability of the virus to storage for 1 week at 4°C. was unaffected by suspension of infected tissue culture fluid in saline, demineralized distilled water, or 5 per cent horse serum in Eagle's basal medium. Heating the virus for 30 minutes at 60°C. had no effect on infectivity and hemagglutination titers. Heating at 65°C. for 30 minutes produced a 3 log unit loss of infectivity and a 4-fold decline in HA titer, and heating at 70°C. for 30 minutes usually produced complete inactivation of infectivity and HA. The virus was resistant to ultraviolet irradiation; ultraviolet irradiation for 2 hours caused a 4 log unit decrease in infectivity titer without affecting the HA titer. The virus was resistant to exposure to 2 per cent phenol and 50 per cent ethyl alcohol but was inactivated by 100 per cent ethyl alcohol, and ethyl alcohol-iodine mixtures. Lyophilization had no effect on the stability of the virus.

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