Cellulose acetate entrapment of Escherichia coli on cotton cloth for aspartate production

1986 ◽  
Vol 8 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Sushama Joshi ◽  
Hiroshi Yamazaki
Keyword(s):  
Escherichia Coli ◽  
Cellulose Acetate ◽  
Cotton Cloth

Effect of TiO2 Loading, Water Depth and Light Intensity on Photo-Disinfection Efficacy of Escherichia Coli O157:H7 Using TiO2 NP-Embedded Cellulose Acetate Film in Water

2021 ◽  
Vol 37 (1) ◽  
pp. 1-9
Author(s):  
Jing Xie ◽  
Yen-Con Hung
Keyword(s):  
Escherichia Coli ◽  
Light Intensity ◽  
Cellulose Acetate ◽  
Water Depth ◽  
Coefficient Of Determination ◽  
List Type ◽  
Water Safety ◽  
Operational Parameters ◽  
Bacterial Reduction ◽  
E Coli

HighlightsPhoto-disinfection using TiO2 NP-embedded CA film showed great potential.TiO2 loading, water depth, and light intensity affected TiO2 photo-disinfection.Bacterial reduction can be predicted based on water depth and light intensity.Durability of the TiO2 NP-embedded CA film has been demonstrated.Abstract. Photocatalysis disinfection has great potential for food and water safety applications. TiO2 NP-embedded cellulose acetate (CA) film has shown effectiveness in inactivating Escherichia coli (E. coli) O157:H7 in water. This study evaluated the effect of several operational parameters such as TiO2 load, light intensity, and water depth on photo-disinfection efficacy of TiO2 NP-embedded CA film. Effect of TiO2 load on photocatalysis disinfection efficacy was investigated by assessing the disinfection rate of the films at different TiO2 levels in inoculated water at a weak UV-A light intensity. The individual and interaction effects of light intensity and water depth on photocatalysis disinfection were evaluated using the response surface methodology (RSM). Based on a central composite design, 13 runs of experiments were conducted with the combination of the two factors at three levels. The effect of film size on photo-disinfection efficacy was also investigated. Results showed that CA film with a TiO2 load of 0.82 mg/cm2 yielded the highest disinfection rate. An empirical model was established to predict the effect of light intensity and water depth on bacterial reduction with a high coefficient of determination (R2 = 0.98). An increased water depth or a decreased light intensity reduced photo-inactivation efficacy. A 5 log reduction was achieved during a 3-h treatment at a light intensity of 1 mW/cm2 and water depth at 1.6 cm. The photo-disinfection efficacy of the film was not significantly (p>0.05) affected by film size. The CA film was able to maintain antimicrobial efficacy after four repeated uses. Results from this study demonstrated that UV-A assisted photo-disinfection using TiO2 NP-embedded CA film has great potential to inactivate pathogens in water. Keywords: Cellulose Acetate, E. coli O157:H7, Photocatalysis, TiO2 nanoparticles, Water Treatment.

A RIBOSOMAL PEPTIDASE FROM ESCHERICHIA COLI B

1965 ◽  
Vol 43 (3) ◽  
pp. 323-329 ◽  
Author(s):  
A. T. Matheson ◽  
C. S. Tsai
Keyword(s):  
Escherichia Coli ◽  
Enzyme Activity ◽  
Ribosomal Protein ◽  
Cellulose Acetate ◽  
Density Gradient ◽  
Sucrose Density Gradient ◽  
E Coli ◽  
Ribosome Structure ◽  
Escherichia Coli B

Properties of a peptidase present in E. coli ribosomes have been studied. The enzyme is tightly bound to the ribosomes, as indicated by repeated washings and centrifugations, sucrose density gradient centrifugations, and electrophoresis on cellulose acetate. The level of enzyme activity in the 30 S particles is twice that found in the 50 S particles. When the ribosome structure is disrupted by enzymic or chemical means, the peptidase behaves similarly to the bulk of the ribosomal protein.

scholarly journals Cellulose Acetate Containing Toluidine Blue and Rose Bengal Is an Effective Antimicrobial Coating when Exposed to White Light

2006 ◽  
Vol 72 (6) ◽  
pp. 4436-4439 ◽  
Author(s):  
Val�rie Decraene ◽  
Jonathan Pratten ◽  
Michael Wilson
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Clostridium Difficile ◽  
Cellulose Acetate ◽  
Toluidine Blue ◽  
White Light ◽  
Rose Bengal ◽  
Microbial Load ◽  
Hospital Associated Infections

ABSTRACT Simple methods of reducing the microbial load on surfaces in hospitals are needed to reduce the risk of hospital-associated infections. Here we report on the ability of a cellulose acetate coating containing the photosensitizers toluidine blue and rose bengal to kill microbes (Staphylococcus aureus, Escherichia coli, Clostridium difficile, a bacteriophage, and Candida albicans) on its surface when illuminated with white light.

scholarly journals Invitro filtration efficiency for selected face masks to bacteria with a size smaller than SARS-CoV-2 respiratory droplet

2020 ◽  
Author(s):  
Maryam Amour ◽  
Hussein H. Mwanga ◽  
George M. Bwire
Keyword(s):  
Escherichia Coli ◽  
Double Layer ◽  
Ambient Air ◽  
Filtration Efficiency ◽  
Cotton Cloth ◽  
Colony Forming Units ◽  
Spread Of Infection ◽  
N95 Respirators ◽  
And Control ◽  
Respiratory Droplets

Abstract Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) can be transmitted between people through respiratory droplets (droplet particles are >5-10 μm in diameter). We conducted an invitro experiment to determine the filtration efficiency for selected face masks (cloth and medical face masks and N95 respirators) to bacteria with a size (0.5-1.5 μm in diameter) smaller than SARS-CoV-2 respiratory droplet. Bacteria suspension was prepared using normal saline (NaCl) and bacteria (Staphylococcus aureus and Escherichia coli) and maintained at a turbidity of 0.5 MacFarland. The suspensions was put in a 100ml plastic spray bottle (with an approximated 250 μl and flow rate of 31.5 ft3/min per spray) and then a single spray was performed to the test masks. Swabbing was done to unsprayed side of the test mask within 0 and after 4 hrs. The swab was streaked on CLED media then incubated for 48 hours at 37oC in ambient air. Bacterial filtration efficiency (BFE) was determined as the proportions of colony forming units (CFUs) between the test and control mask. The selected face masks had BFE of 100% and >99% for medical and double layer cotton cloth masks, respectively. This study supports the use of cotton cloth (at least double layer) face coverings in public settings where other social distancing measures are difficult to maintain to prevent the spread of infection from the wearer.

Antibacterial Activity of Photoactive Silk Fibroin/Cellulose Acetate Blend Nanofibrous Membranes against Escherichia coli

2020 ◽  
Vol 8 (45) ◽  
pp. 16775-16780
Author(s):  
Shixiong Yi ◽  
Yuehan Wu ◽  
Yuansong Zhang ◽  
Yushan Zou ◽  
Fangyin Dai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Cellulose Acetate ◽  
Silk Fibroin ◽  
Nanofibrous Membranes

scholarly journals Cellulose Acetate Membranes Modification by Aminosilane Grafting in Supercritical Carbon Dioxide towards Antibiofilm Properties

Membranes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Marcin Tyrka ◽  
Mariusz Nowak ◽  
Dusan Misic ◽  
Tomasz Półbrat ◽  
Stanisław Koter ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Staphylococcus Aureus ◽  
Supercritical Carbon Dioxide ◽  
Cellulose Acetate ◽  
Salmonella Enteritidis ◽  
Reaction Medium ◽  
Grafting Reaction ◽  
Cellulose Acetate Membranes ◽  
Supercritical Carbon

The study explores the grafting of cellulose acetate microfiltration membranes with an aminosilane to attain antibiofilm properties. The grafting reaction was performed in the supercritical carbon dioxide used as a transport and reaction medium. The FTIR analyses and dissolution tests confirmed the covalent bonding between the aminosilane and polymer. The membranes’ microstructure was investigated using a dual-beam SEM and ion microscopy, and no adverse effects of the processing were found. The modified membranes showed a more hydrophilic nature and larger water permeate flow rate than the neat cellulose acetate membranes. The tests in a cross-filtration unit showed that modified membranes were considerably less blocked after a week of exposure to Staphylococcus aureus and Escherichia coli than the original ones. Microbiological investigations revealed strong antibiofilm properties of the grafted membranes in experiments with Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella Enteritidis.

scholarly journals In Vitro Biocontrol of Escherichia coli Through the Immobilization of its Specific Lytic Bacteriophage on Cellulose Acetate Biodegradable Film

2019 ◽  
Vol 12 (6) ◽  
pp. 399-408
Author(s):  
Samaneh Faraji Kafshgari ◽  
Yahya Maghsoudlou ◽  
Morteza Khomeyri ◽  
Mahboubeh Kashiri ◽  
Arash Babaei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cellulose Acetate ◽  
Lytic Bacteriophage ◽  
Biodegradable Film

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

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