Unusual solvent effect on protease activity and effective optical resolution of amino acids by hydrolytic reactions in organic solvents

1991 ◽  
Vol 13 (5) ◽  
pp. 317-322 ◽  
Author(s):  
Hideo Kise ◽  
Yoshimasa Tomiuchi
Keyword(s):  
Amino Acids ◽  
Solvent Effect ◽  
Organic Solvents ◽  
Protease Activity ◽  
Optical Resolution

scholarly journals Effect of Time and Legume Type on Germination-Induced Proteolysis of Lentils and Faba Beans

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 4
Author(s):  
Sara Bautista-Expósito ◽  
Elena Peñas ◽  
Albert Vanderberg ◽  
Juana Frias ◽  
Cristina Martínez-Villaluenga
Keyword(s):  
Amino Acids ◽  
Protease Activity ◽  
Protein Quality ◽  
Principal Component ◽  
Fold Increase ◽  
Protein Digestibility ◽  
Essential Amino Acids ◽  
Seedling Development ◽  
Positive Effects ◽  
Faba Beans

Legumes are alternative protein sources that have been successfully used to develop diverse meatless foods. Although these plant-based products have a lower impact on the environment than equivalent animal-based products, they have lower protein digestibility. Germination could be a useful bioprocess to enhance protein digestibility in legumes, although its effect at different times of seedling development has been little studied in lentils and faba beans. This work investigated the effect of germination time (4 and 6 days after full seed imbibition) on the proteins of three types of Canadian lentils (“gray zero tannin”, G; “caviar black”, B; and “red dehulled”, D) and faba beans (“zero vicin/convicin”, F). Germination increased total nitrogen (4–14% increase) and total levels of some amino acids: Asp in all the sprouts studied; Ser, Pro, Ala, Cys, His and Lys in G; and Met and Tyr in B. A concurrent degradation of the 7S and 11S globulin subunits, the accumulation of peptides below 20 kDa and free essential and non-essential amino acids (4- to 6-fold increase) were observed after germination in all the legumes studied. These effects were attributable to the increased protease activity observed after sprouting. Trypsin inhibitory activity was lower in legume sprouts, except for D, where a small increase was detected. Time, legume type and their interaction showed significant effects on the parameters studied. Germination effects were generally more remarkable at longer stages of seedling development. Among the legumes studied, D showed a differential behavior characterized by a faster protein degradation and release of small peptides, probably due to its higher protease activity as indicated by principal component analysis. These results evidence the positive effects of germination on the protein digestibility of different lentil types and faba beans. The protein quality of plant-based foods could be improved through the selection of legume species with higher germination-induced proteolytic rates and optimized germination times.

The water-propanol solvent effect on the reactivity of α-amino acids and dipeptides in the N-acylation with picryl benzoate

2012 ◽  
Vol 82 (11) ◽  
pp. 1805-1809
Author(s):  
L. V. Kuritsin ◽  
L. B. Kochetova ◽  
N. V. Kalinina ◽  
T. P. Kustova
Keyword(s):  
Amino Acids ◽  
Solvent Effect

scholarly journals Studies on Hydroxy Amino Acids. II. The Optical Resolution of α-Amino-β-hydroxy-γ-benzyloxybutyric Acid

1969 ◽  
Vol 42 (9) ◽  
pp. 2720-2722 ◽  
Author(s):  
Kenji Okawa ◽  
Kiyotaka Hori ◽  
Katsutoshi Hirose ◽  
Yasuo Nakagawa
Keyword(s):  
Amino Acids ◽  
Optical Resolution ◽  
Hydroxy Amino

Molecularly Imprinted Polymeric Membranes Containing DIDE Derivatives for Optical Resolution of Amino Acids†

1996 ◽  
Vol 29 (25) ◽  
pp. 8197-8203 ◽  
Author(s):  
Masakazu Yoshikawa ◽  
Jun-ichiro Izumi ◽  
Toshio Kitao ◽  
Shunji Sakamoto
Keyword(s):  
Amino Acids ◽  
Optical Resolution ◽  
Polymeric Membranes ◽  
Molecularly Imprinted

Preparation of Thin Film Polymer Composite Membranes for Optical Resolution of Racemic Mixture of α-Amino Acids

2013 ◽  
Vol 48 (12) ◽  
pp. 1777-1787 ◽  
Author(s):  
Pravin G. Ingole ◽  
Hari C. Bajaj ◽  
D. N. Srivastava ◽  
Babulal Rebary ◽  
Kripal Singh
Keyword(s):  
Thin Film ◽  
Amino Acids ◽  
Polymer Composite ◽  
Optical Resolution ◽  
Composite Membranes ◽  
Racemic Mixture ◽  
Film Polymer
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