Changes in erythrocyte carbonic anhydrase activity due to physical exercise

1984 ◽  
Vol 52 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Shuji Tokuda ◽  
Akira Iiboshi ◽  
Masaharu Suenaga ◽  
Shogo Otsuji
Keyword(s):  
Carbonic Anhydrase ◽  
Physical Exercise ◽  
Carbonic Anhydrase Activity ◽  
Erythrocyte Carbonic Anhydrase

An evaluation of cytosolic erythrocyte carbonic anhydrase and catalase in carcinoma patients: An elevation of carbonic anhydrase activity

2006 ◽  
Vol 39 (8) ◽  
pp. 804-809 ◽  
Author(s):  
Ozen Ozensoy ◽  
Feray Kockar ◽  
Oktay Arslan ◽  
Semra Isik ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Activity ◽  
Erythrocyte Carbonic Anhydrase

Effect of Physical Exercise on Erythrocyte Carbonic Anhydrase Isozymes and 2,3-Diphosphoglycerate in Men

1981 ◽  
Vol 02 (04) ◽  
pp. 231-235 ◽  
Author(s):  
H. Ohno ◽  
N. Taniguchi ◽  
T. Kondo ◽  
K. Terayama ◽  
F. Hirata ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Physical Exercise ◽  
Erythrocyte Carbonic Anhydrase

scholarly journals Enrichment of erythrocytes of fetal origin from adult-fetal blood mixtures via selective hemolysis of adult blood cells: an aid to antenatal diagnosis of hemoglobinopathies

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 883-897
Author(s):  
SH Boyer ◽  
AN Noyes ◽  
ML Boyer
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Inhibitor ◽  
Antenatal Diagnosis ◽  
Carbonic Anhydrase Activity ◽  
Red Cells ◽  
Fetal Origin ◽  
Successful Isolation ◽  
Erythrocyte Enzyme ◽  
Erythrocyte Carbonic Anhydrase ◽  
Carbonic Anhydrase Enzyme

Red cell lysis in isotonic solutions containing NH4Cl, NH4HCO3, and a carbonic anhydrase enzyme inhibitor (acetazolamide) is a function of erythrocyte enzyme activity and permeability of cells to the inhibitor. Erythrocyte carbonic anhydrase activity is at least fivefold greater and acetazolamide permeability about tenfold less for adults than for newborns. In this setting, greater than 99.9% of red cells from adults can be hemolyzed at a time when greater than 25% of those from newborns remain intact. This easily applied method may be useful when antenatal diagnosis of hemoglobinopathies is otherwise precluded by contaimination with maternal erythrocytes. The feasibility of differential hemolysis via NH4Cl--HCO3-mediated, acetazolamide- modulated reactions is shown by the successful isolation of the few fetal-origin erythrocytes present in grossly nonbloody amniotic fluids and, in one instance, by approximately 3300-fold enrichment of apparently authentic fetal-origin red cells from the arm blood of a woman in her 18th wk of pregnancy.

scholarly journals Carbonic anhydrase, ultrastructural localization in the mouse gastric mucosa and improvements in the technique.

1980 ◽  
Vol 28 (6) ◽  
pp. 511-525 ◽  
Author(s):  
N Sugai ◽  
S Ito
Keyword(s):  
Electron Microscopy ◽  
Gastric Mucosa ◽  
Carbonic Anhydrase ◽  
Picric Acid ◽  
Ultrastructural Localization ◽  
Carbonic Anhydrase Activity ◽  
Cobalt Sulfide ◽  
Ultrastructural Studies ◽  
Mucosal Cells ◽  
Erythrocyte Carbonic Anhydrase

The ultrastructural localization of carbonic anhydrase activity in mouse gastric mucosal cells as revealed by the cobalt bicarbonate histochemical method of Hansson has been made. In addition the effects of fixatives used for ultrastructural studies have been evaluated for reduction of carbonic anhydrase activity; exogenous erythrocyte carbonic anhydrase has been localized in tissues; acetazolamide and potassium cyanate inhibition of activity demonstrated; and an improved method for the osmication of reacted tissues for electron microscopy has been developed. The results indicate that the glutaraldehyde, formaldehyde, picric acid fixative, which retains about 5% of the original carbonic anhydrase activity, is distinctly better for histochemical studies than formaldehyde fixation, which retains about 32% activity. Acetazolamide at 10(-5) M consistently inhibits histochemical reaction, as does 20 mM KCNO, in the incubation medium. Exogenous carbonic anhydrase is readily visualized by the histochemical technique. Electron microscopy of gastric mucosa reacted for carbonic anhydrase activity indicates the focal deposition of the cobalt sulfide reaction product in the cores of microvilli lining the intracellular canaliculi, in the basal and lateral cell folds of parietal cells, and in the microvilli as well as the cytoplasm between mucous granules in the surface mucous cells. In addition, some reaction product was found in the mitochondrial cristae and in some nuclei and intercellular spaces.

Erythrocyte carbonic anhydrase activity in smokers and in diabetic patients

2009 ◽  
Vol 105 (S 02) ◽  
pp. 17-19 ◽  
Author(s):  
P. Abel ◽  
S. Wussow ◽  
H. Blücher ◽  
G. Gros ◽  
R. Rettig ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Activity ◽  
Diabetic Patients ◽  
Erythrocyte Carbonic Anhydrase

scholarly journals Enrichment of erythrocytes of fetal origin from adult-fetal blood mixtures via selective hemolysis of adult blood cells: an aid to antenatal diagnosis of hemoglobinopathies

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 883-897 ◽  
Author(s):  
SH Boyer ◽  
AN Noyes ◽  
ML Boyer
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Inhibitor ◽  
Antenatal Diagnosis ◽  
Carbonic Anhydrase Activity ◽  
Red Cells ◽  
Fetal Origin ◽  
Successful Isolation ◽  
Erythrocyte Enzyme ◽  
Erythrocyte Carbonic Anhydrase ◽  
Carbonic Anhydrase Enzyme

Abstract Red cell lysis in isotonic solutions containing NH4Cl, NH4HCO3, and a carbonic anhydrase enzyme inhibitor (acetazolamide) is a function of erythrocyte enzyme activity and permeability of cells to the inhibitor. Erythrocyte carbonic anhydrase activity is at least fivefold greater and acetazolamide permeability about tenfold less for adults than for newborns. In this setting, greater than 99.9% of red cells from adults can be hemolyzed at a time when greater than 25% of those from newborns remain intact. This easily applied method may be useful when antenatal diagnosis of hemoglobinopathies is otherwise precluded by contaimination with maternal erythrocytes. The feasibility of differential hemolysis via NH4Cl--HCO3-mediated, acetazolamide- modulated reactions is shown by the successful isolation of the few fetal-origin erythrocytes present in grossly nonbloody amniotic fluids and, in one instance, by approximately 3300-fold enrichment of apparently authentic fetal-origin red cells from the arm blood of a woman in her 18th wk of pregnancy.

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