Fixation and staining of granules in mucosal mast cells and intraepithelial lymphocytes in the rat jejunum, with special reference to the relationship between the acid glycosaminoglycans in the two cell types

1980 ◽  
Vol 12 (5) ◽  
pp. 513-526 ◽  
Author(s):  
Graham Mayrhofer
Keyword(s):  
Mast Cells ◽  
Special Reference ◽  
Cell Types ◽  
Intraepithelial Lymphocytes ◽  
Rat Jejunum ◽  
Mucosal Mast Cells ◽  
The Relationship ◽  
Acid Glycosaminoglycans

scholarly journals Thymus-dependent and thymus-independent subpopulations of intestinal intraepithelial lymphocytes: a granular subpopulation of probable bone marrow origin and relationship to mucosal mast cells

Blood ◽  
1980 ◽  
Vol 55 (3) ◽  
pp. 532-535 ◽  
Author(s):  
G Mayrhofer
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Granular Cell ◽  
Intraepithelial Lymphocytes ◽  
The Other ◽  
Intestinal Intraepithelial Lymphocytes ◽  
Mucosal Mast Cells ◽  
Bone Marrow Origin ◽  
Small Intestines ◽  
Rats And Mice

Intraepithelial lymphocytes have been examined histochemically in the small intestines of normal rats and mice and in thymus-deficient animals (B rats and nude mice). It is concluded that these cells are heterogeneous, consisting of at least two subpopulations. One population contains granules, is thymus-independent, and is probably bone-marrow-derived. The other population does not contain granules and appears to be thymus-dependent. It is suggested that the granular cell, which may be a precursor of mucosal mast cells, is more properly a previously unrecognized nonlymphoid leukocyte of bone marrow origin.

scholarly journals 5 MUCOSAL MAST CELLS IN THE BABY RAT JEJUNUM

1988 ◽  
Vol 24 (3) ◽  
pp. 406-406
Author(s):  
M Varkasalo ◽  
A Fergucon ◽  
H R P Millor ◽  
A Cummins
Keyword(s):  
Mast Cells ◽  
Rat Jejunum ◽  
Mucosal Mast Cells

scholarly journals The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions.

1978 ◽  
Vol 148 (6) ◽  
pp. 1661-1677 ◽  
Author(s):  
D Guy-Grand ◽  
C Griscelli ◽  
P Vassalli
Keyword(s):  
T Cells ◽  
Mast Cells ◽  
T Lymphocytes ◽  
Intestinal Mucosa ◽  
Cell Types ◽  
Intraepithelial Lymphocytes ◽  
Graft Versus Host ◽  
Dividing Cells ◽  
Gut Mucosa ◽  
Chediak Higashi Syndrome

Lymphocytes of the mouse intestinal mucosa, identified in tissue sections or purified suspensions of intraepithelial lymphocytes as T cells (gut T lymphocytes [GTL]), were studied in normal mice or in beige mice (the equivalent of the Chediak-Higashi syndrome in man, characterized by giant granules in various cell types, including mast cells). Mice were studied in normal or in germ-free conditions, or during a graft versus host (GVH) reaction resulting from the injection of parental thymocytes into lethally irradiated F1 mice, a condition leading to massive accumulation of T lymphocytes of donor origin in the host gut mucosa. In normal as well as in GVH conditions, a high percentage of the gut IE lymphocytes contain granules (up to 80% in the beige mouse). These granules have ultrastructural, hostochemical and other features resembling those of mast cell granules; in beige mice, up to 50% of them can be shown to contain histamine. Granulated T cells are also found in the lamina propria. It appears that the GTL may progressively lose their surface T antigens when the granules become more developed. Kinetics of [3H]TdR labeling of the GTL, transfer experiments with T cells of various origins, selective [3H]TdR labeling and selective irradiation of the Peyer's patches (PP), and effect of thoraic duct (TD) drainage led to the conclusion that GTL are the progeny of T cells stimulated to divide in the PP microenvironment, which endows them with a gut-homing tendency. From the PP, these cells follow a cycle, migrating to the TD and to the blood to colonize the whole intestinal mucosa, the majority of them as dividing cells undergoing a single round of traffic, with some probably able to recirculate and becoming a more long-lived variety. Antigenic stimulation within the PP is necessary for the emergence of GTL progenitors, but their gut-homing property is unrelated to the antigen as shown with fetal gut grafts, notably in GVH where grafts syngeneic to the host or donor become similarly infiltrated by GTL. On the basis of their properties and of further evidence to be reported elsewhere, it is proposed that GTL belong to a special class of T lymphocytes, related to the immune defenses of the mucosal systems in general, and capable of acting as progenitors of mucosal mast cells.

scholarly journals Thymus-dependent and thymus-independent subpopulations of intestinal intraepithelial lymphocytes: a granular subpopulation of probable bone marrow origin and relationship to mucosal mast cells

Blood ◽  
1980 ◽  
Vol 55 (3) ◽  
pp. 532-535 ◽  
Author(s):  
G Mayrhofer
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Granular Cell ◽  
Intraepithelial Lymphocytes ◽  
The Other ◽  
Intestinal Intraepithelial Lymphocytes ◽  
Mucosal Mast Cells ◽  
Bone Marrow Origin ◽  
Small Intestines ◽  
Rats And Mice

Abstract Intraepithelial lymphocytes have been examined histochemically in the small intestines of normal rats and mice and in thymus-deficient animals (B rats and nude mice). It is concluded that these cells are heterogeneous, consisting of at least two subpopulations. One population contains granules, is thymus-independent, and is probably bone-marrow-derived. The other population does not contain granules and appears to be thymus-dependent. It is suggested that the granular cell, which may be a precursor of mucosal mast cells, is more properly a previously unrecognized nonlymphoid leukocyte of bone marrow origin.

scholarly journals Mast Cells Interact with Endothelial Cells to Accelerate In Vitro Angiogenesis

2017 ◽  
Author(s):  
Devandir Antonio de Souza Junior ◽  
Vivian Marino Mazucato ◽  
Ana Carolina Santana ◽  
Constance Oliver ◽  
Maria Celia Jamur
Keyword(s):  
Endothelial Cells ◽  
Mast Cells ◽  
Intercellular Communication ◽  
Cell Types ◽  
Tissue Microenvironment ◽  
Complex Process ◽  
The Relationship ◽  
In Vitro Angiogenesis

Angiogenesis is a complex process that involves interactions between endothelial cells and various other cell types as well as the tissue microenvironment. Several previous studies have demonstrated that mast cells accumulate at angiogenic sites. In spite of the evidence suggesting a relationship between mast cells and angiogenesis, the association of mast cells and endothelial cells remains poorly understood. The present study aims to investigate the relationship between mast cells and endothelial cells during in vitro angiogenesis. When endothelial cells were co-cultured with mast cells, angiogenesis was stimulated. Furthermore, there was direct intercellular communication via gap junctions between the two cell types. In addition, the presence of mast cells stimulated endothelial cells to release angiogenic factors. Moreover, conditioned medium from the co-cultures also stimulated in vitro angiogenesis. The results from this investigation demonstrate that mast cells have both direct and indirect proangiogenic effects and provide new insights into the role of mast cells in angiogenesis.

The relationship of IGE receptor topography to signaling activity in RBL-2H3 mast cells

1991 ◽  
Vol 49 ◽  
pp. 236-237
Author(s):  
J.R. Pfeiffer ◽  
J.C. Seagrave ◽  
C. Wofsy ◽  
J.M. Oliver
Keyword(s):  
Mast Cells ◽  
Protein A ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Ige Binding ◽  
Electron Imaging ◽  
Small Clusters ◽  
Relationship Of ◽  
The Relationship

In RBL-2H3 rat leukemic mast cells, crosslinking IgE-receptor complexes with anti-IgE antibody leads to degranulation. Receptor crosslinking also stimulates the redistribution of receptors on the cell surface, a process that can be observed by labeling the anti-IgE with 15 nm protein A-gold particles as described in Stump et al. (1989), followed by back-scattered electron imaging (BEI) in the scanning electron microscope. We report that anti-IgE binding stimulates the redistribution of IgE-receptor complexes at 37“C from a dispersed topography (singlets and doublets; S/D) to distributions dominated sequentially by short chains, small clusters and large aggregates of crosslinked receptors. These patterns can be observed (Figure 1), quantified (Figure 2) and analyzed statistically. Cells incubated with 1 μg/ml anti-IgE, a concentration that stimulates maximum net secretion, redistribute receptors as far as chains and small clusters during a 15 min incubation period. At 3 and 10 μg/ml anti-IgE, net secretion is reduced and the majority of receptors redistribute rapidly into clusters and large aggregates.

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